Detection of classic and invasive E. coli and Shigella serotypes in stools by indirect immunofluorescence
IIF in the detection of invasive and classic enteropathogenic E. coli and Shigella serotypes was compared with traditional coproculture methods. IIP results agreed with the coproculture findings in 128 out of 140 cases tested for enteropathogenic E. coli (91%) and in 108 out of 112 for Shigella (96%...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Universidade de São Paulo (USP)
1984
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S0036-46651984000600002 https://doaj.org/article/b98be0d12b0340b1aa3a0271145144bc |
id |
ftdoajarticles:oai:doaj.org/article:b98be0d12b0340b1aa3a0271145144bc |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:b98be0d12b0340b1aa3a0271145144bc 2024-09-09T19:25:11+00:00 Detection of classic and invasive E. coli and Shigella serotypes in stools by indirect immunofluorescence Waldely de Oliveira Dias Octávio Augusto de Carvalho Pereira 1984-12-01T00:00:00Z https://doi.org/10.1590/S0036-46651984000600002 https://doaj.org/article/b98be0d12b0340b1aa3a0271145144bc EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651984000600002&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651984000600002 https://doaj.org/article/b98be0d12b0340b1aa3a0271145144bc Revista do Instituto de Medicina Tropical de São Paulo, Vol 26, Iss 6, Pp 295-300 (1984) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1984 ftdoajarticles https://doi.org/10.1590/S0036-46651984000600002 2024-08-05T17:49:30Z IIF in the detection of invasive and classic enteropathogenic E. coli and Shigella serotypes was compared with traditional coproculture methods. IIP results agreed with the coproculture findings in 128 out of 140 cases tested for enteropathogenic E. coli (91%) and in 108 out of 112 for Shigella (96%). All cases with positive reactions by coproculture were confirmed by IIP. In the control group it were obtained by IIF 12 cases with positive reactions for enteropathogenic E. coli and 4 cases for Shigella, including two cases of mixed infection by E. coli 026/Sh. dysenteriae and E. coli 0124/Sh. dysenteriae. It was discussed the high sensitivity and specificity of the IIF when compared with the traditional methods, being suggested that IIF is a valuable tool in epidemiological studies involving these organisms and an important aid in the stablishment of an early presumptive diagnosis of the acute infantile diarrhea. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 26 6 295 300 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Waldely de Oliveira Dias Octávio Augusto de Carvalho Pereira Detection of classic and invasive E. coli and Shigella serotypes in stools by indirect immunofluorescence |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
IIF in the detection of invasive and classic enteropathogenic E. coli and Shigella serotypes was compared with traditional coproculture methods. IIP results agreed with the coproculture findings in 128 out of 140 cases tested for enteropathogenic E. coli (91%) and in 108 out of 112 for Shigella (96%). All cases with positive reactions by coproculture were confirmed by IIP. In the control group it were obtained by IIF 12 cases with positive reactions for enteropathogenic E. coli and 4 cases for Shigella, including two cases of mixed infection by E. coli 026/Sh. dysenteriae and E. coli 0124/Sh. dysenteriae. It was discussed the high sensitivity and specificity of the IIF when compared with the traditional methods, being suggested that IIF is a valuable tool in epidemiological studies involving these organisms and an important aid in the stablishment of an early presumptive diagnosis of the acute infantile diarrhea. |
format |
Article in Journal/Newspaper |
author |
Waldely de Oliveira Dias Octávio Augusto de Carvalho Pereira |
author_facet |
Waldely de Oliveira Dias Octávio Augusto de Carvalho Pereira |
author_sort |
Waldely de Oliveira Dias |
title |
Detection of classic and invasive E. coli and Shigella serotypes in stools by indirect immunofluorescence |
title_short |
Detection of classic and invasive E. coli and Shigella serotypes in stools by indirect immunofluorescence |
title_full |
Detection of classic and invasive E. coli and Shigella serotypes in stools by indirect immunofluorescence |
title_fullStr |
Detection of classic and invasive E. coli and Shigella serotypes in stools by indirect immunofluorescence |
title_full_unstemmed |
Detection of classic and invasive E. coli and Shigella serotypes in stools by indirect immunofluorescence |
title_sort |
detection of classic and invasive e. coli and shigella serotypes in stools by indirect immunofluorescence |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1984 |
url |
https://doi.org/10.1590/S0036-46651984000600002 https://doaj.org/article/b98be0d12b0340b1aa3a0271145144bc |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 26, Iss 6, Pp 295-300 (1984) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651984000600002&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46651984000600002 https://doaj.org/article/b98be0d12b0340b1aa3a0271145144bc |
op_doi |
https://doi.org/10.1590/S0036-46651984000600002 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
26 |
container_issue |
6 |
container_start_page |
295 |
op_container_end_page |
300 |
_version_ |
1809894961850613760 |