Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens.
Brucellosis is a widespread zoonotic disease that is also a potential agent of bioterrorism. Current serological assays to diagnose human brucellosis in clinical settings are based on detection of agglutinating anti-LPS antibodies. To better understand the universe of antibody responses that develop...
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ftdoajarticles:oai:doaj.org/article:b980c058ec514e9ba85c91ef39520864 2023-05-15T15:07:54+02:00 Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens. Li Liang Diana Leng Chad Burk Rie Nakajima-Sasaki Matthew A Kayala Vidya L Atluri Jozelyn Pablo Berkay Unal Thomas A Ficht Eduardo Gotuzzo Mayuko Saito W John W Morrow Xiaowu Liang Pierre Baldi Robert H Gilman Joseph M Vinetz Renée M Tsolis Philip L Felgner 2010-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000673 https://doaj.org/article/b980c058ec514e9ba85c91ef39520864 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2864264?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000673 https://doaj.org/article/b980c058ec514e9ba85c91ef39520864 PLoS Neglected Tropical Diseases, Vol 4, Iss 5, p e673 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000673 2022-12-31T00:25:42Z Brucellosis is a widespread zoonotic disease that is also a potential agent of bioterrorism. Current serological assays to diagnose human brucellosis in clinical settings are based on detection of agglutinating anti-LPS antibodies. To better understand the universe of antibody responses that develop after B. melitensis infection, a protein microarray was fabricated containing 1,406 predicted B. melitensis proteins. The array was probed with sera from experimentally infected goats and naturally infected humans from an endemic region in Peru. The assay identified 18 antigens differentially recognized by infected and non-infected goats, and 13 serodiagnostic antigens that differentiate human patients proven to have acute brucellosis from syndromically similar patients. There were 31 cross-reactive antigens in healthy goats and 20 cross-reactive antigens in healthy humans. Only two of the serodiagnostic antigens and eight of the cross-reactive antigens overlap between humans and goats. Based on these results, a nitrocellulose line blot containing the human serodiagnostic antigens was fabricated and applied in a simple assay that validated the accuracy of the protein microarray results in the diagnosis of humans. These data demonstrate that an experimentally infected natural reservoir host produces a fundamentally different immune response than a naturally infected accidental human host. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 5 e673 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Li Liang Diana Leng Chad Burk Rie Nakajima-Sasaki Matthew A Kayala Vidya L Atluri Jozelyn Pablo Berkay Unal Thomas A Ficht Eduardo Gotuzzo Mayuko Saito W John W Morrow Xiaowu Liang Pierre Baldi Robert H Gilman Joseph M Vinetz Renée M Tsolis Philip L Felgner Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Brucellosis is a widespread zoonotic disease that is also a potential agent of bioterrorism. Current serological assays to diagnose human brucellosis in clinical settings are based on detection of agglutinating anti-LPS antibodies. To better understand the universe of antibody responses that develop after B. melitensis infection, a protein microarray was fabricated containing 1,406 predicted B. melitensis proteins. The array was probed with sera from experimentally infected goats and naturally infected humans from an endemic region in Peru. The assay identified 18 antigens differentially recognized by infected and non-infected goats, and 13 serodiagnostic antigens that differentiate human patients proven to have acute brucellosis from syndromically similar patients. There were 31 cross-reactive antigens in healthy goats and 20 cross-reactive antigens in healthy humans. Only two of the serodiagnostic antigens and eight of the cross-reactive antigens overlap between humans and goats. Based on these results, a nitrocellulose line blot containing the human serodiagnostic antigens was fabricated and applied in a simple assay that validated the accuracy of the protein microarray results in the diagnosis of humans. These data demonstrate that an experimentally infected natural reservoir host produces a fundamentally different immune response than a naturally infected accidental human host. |
format |
Article in Journal/Newspaper |
author |
Li Liang Diana Leng Chad Burk Rie Nakajima-Sasaki Matthew A Kayala Vidya L Atluri Jozelyn Pablo Berkay Unal Thomas A Ficht Eduardo Gotuzzo Mayuko Saito W John W Morrow Xiaowu Liang Pierre Baldi Robert H Gilman Joseph M Vinetz Renée M Tsolis Philip L Felgner |
author_facet |
Li Liang Diana Leng Chad Burk Rie Nakajima-Sasaki Matthew A Kayala Vidya L Atluri Jozelyn Pablo Berkay Unal Thomas A Ficht Eduardo Gotuzzo Mayuko Saito W John W Morrow Xiaowu Liang Pierre Baldi Robert H Gilman Joseph M Vinetz Renée M Tsolis Philip L Felgner |
author_sort |
Li Liang |
title |
Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens. |
title_short |
Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens. |
title_full |
Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens. |
title_fullStr |
Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens. |
title_full_unstemmed |
Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens. |
title_sort |
large scale immune profiling of infected humans and goats reveals differential recognition of brucella melitensis antigens. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000673 https://doaj.org/article/b980c058ec514e9ba85c91ef39520864 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 5, p e673 (2010) |
op_relation |
http://europepmc.org/articles/PMC2864264?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000673 https://doaj.org/article/b980c058ec514e9ba85c91ef39520864 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000673 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
4 |
container_issue |
5 |
container_start_page |
e673 |
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1766339333754716160 |