Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico.
Background The progressive disseminated histoplasmosis (PDH) has been associated with severe disease and high risk of death among people living with HIV (PLWHIV). Therefore, the purpose of this multicenter, prospective, double-blinded study done in ten Mexican hospitals was to determine the diagnost...
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2021
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Online Access: | https://doi.org/10.1371/journal.pntd.0009215 https://doaj.org/article/b93579871f1a49d1a1f7166df3e2a1d2 |
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ftdoajarticles:oai:doaj.org/article:b93579871f1a49d1a1f7166df3e2a1d2 2023-05-15T15:18:24+02:00 Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico. Areli Martínez-Gamboa María Dolores Niembro-Ortega Pedro Torres-González Janeth Santiago-Cruz Nancy Guadalupe Velázquez-Zavala Andrea Rangel-Cordero Brenda Crabtree-Ramírez Armando Gamboa-Domínguez Edgardo Reyes-Gutiérrez Gustavo Reyes-Terán Víctor Hugo Lozano-Fernandez Víctor Hugo Ahumada-Topete Pedro Martínez-Ayala Marisol Manríquez-Reyes Juan Pablo Ramírez-Hinojosa Patricia Rodríguez-Zulueta Christian Hernández-León Jesús Ruíz-Quiñones Norma Eréndira Rivera-Martínez Alberto Chaparro-Sánchez Jaime Andrade-Villanueva Luz Alicia González-Hernández Sofia Cruz-Martínez Oscar Flores-Barrientos Jesús Enrique Gaytán-Martínez Martín Magaña-Aquino Axel Cervantes-Sánchez Antonio Olivas-Martínez Javier Araujo-Meléndez María Del Rocío Reyes-Montes Esperanza Duarte-Escalante María Guadalupe Frías-De León José Antonio Ramírez María Lucia Taylor Alfredo Ponce de León-Garduño José Sifuentes-Osornio 2021-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009215 https://doaj.org/article/b93579871f1a49d1a1f7166df3e2a1d2 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009215 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009215 https://doaj.org/article/b93579871f1a49d1a1f7166df3e2a1d2 PLoS Neglected Tropical Diseases, Vol 15, Iss 3, p e0009215 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009215 2022-12-31T06:00:24Z Background The progressive disseminated histoplasmosis (PDH) has been associated with severe disease and high risk of death among people living with HIV (PLWHIV). Therefore, the purpose of this multicenter, prospective, double-blinded study done in ten Mexican hospitals was to determine the diagnostic accuracy of detecting Histoplasma capsulatum antigen in urine using the IMMY ALPHA Histoplasma EIA kit (IAHE), clarus Histoplasma GM Enzyme Immunoassay (cHGEI IMMY) and MiraVista Histoplasma Urine Antigen LFA (MVHUALFA); as well as the Hcp100 and 1281-1283220SCAR nested PCRs in blood, bone-marrow, tissue biopsies and urine. Methodology/principal findings We included 415 PLWHIV older than 18 years of age with suspicion of PDH. Using as diagnostic standard recovery of H. capsulatum in blood, bone marrow or tissue cultures, or histopathological exam compatible, detected 108 patients (26%, [95%CI, 21.78-30.22]) with proven-PDH. We analyzed 391 urine samples by the IAHE, cHGEI IMMY and MVHUALFA; the sensitivity/specificity values obtained were 67.3% (95% CI, 57.4-76.2) / 96.2% (95% CI, 93.2-98.0) for IAHE, 91.3% (95% CI, 84.2-96.0) / 90.9% (95% CI, 87.0-94.0) for cHGEI IMMY and 90.4% (95% CI, 83.0-95.3) / 92.3% (95% CI, 88.6-95.1) for MVHUALFA. The Hcp100 nested PCR was performed on 393, 343, 75 and 297, blood, bone marrow, tissue and urine samples respectively; the sensitivity/specificity values obtained were 62.9% (95%CI, 53.3-72.5)/ 89.5% (95%CI, 86.0-93.0), 65.9% (95%CI, 56.0-75.8)/ 89.0% (95%CI, 85.2-92.9), 62.1% (95%CI, 44.4-79.7)/ 82.6% (95%CI, 71.7-93.6) and 34.9% (95%CI, 24.8-46.2)/ 67.3% (95%CI, 60.6-73.5) respectively; and 1281-1283220SCAR nested PCR was performed on 392, 344, 75 and 291, respectively; the sensitivity/specificity values obtained were 65.3% (95% CI, 55.9-74.7)/ 58.8% (95%CI, 53.2-64.5), 70.8% (95%CI, 61.3-80.2)/ 52.9% (95%CI, 46.8-59.1), 71.4% (95%CI, 54.7-88.2)/ 40.4% (95%CI, 26.4-54.5) and 18.1% (95%CI, 10.5-28.1)/ 90.4% (95%CI, 85.5-94.0), respectively. Conclusions/significance The cHGEI ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Eia ENVELOPE(7.755,7.755,63.024,63.024) PLOS Neglected Tropical Diseases 15 3 e0009215 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Areli Martínez-Gamboa María Dolores Niembro-Ortega Pedro Torres-González Janeth Santiago-Cruz Nancy Guadalupe Velázquez-Zavala Andrea Rangel-Cordero Brenda Crabtree-Ramírez Armando Gamboa-Domínguez Edgardo Reyes-Gutiérrez Gustavo Reyes-Terán Víctor Hugo Lozano-Fernandez Víctor Hugo Ahumada-Topete Pedro Martínez-Ayala Marisol Manríquez-Reyes Juan Pablo Ramírez-Hinojosa Patricia Rodríguez-Zulueta Christian Hernández-León Jesús Ruíz-Quiñones Norma Eréndira Rivera-Martínez Alberto Chaparro-Sánchez Jaime Andrade-Villanueva Luz Alicia González-Hernández Sofia Cruz-Martínez Oscar Flores-Barrientos Jesús Enrique Gaytán-Martínez Martín Magaña-Aquino Axel Cervantes-Sánchez Antonio Olivas-Martínez Javier Araujo-Meléndez María Del Rocío Reyes-Montes Esperanza Duarte-Escalante María Guadalupe Frías-De León José Antonio Ramírez María Lucia Taylor Alfredo Ponce de León-Garduño José Sifuentes-Osornio Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background The progressive disseminated histoplasmosis (PDH) has been associated with severe disease and high risk of death among people living with HIV (PLWHIV). Therefore, the purpose of this multicenter, prospective, double-blinded study done in ten Mexican hospitals was to determine the diagnostic accuracy of detecting Histoplasma capsulatum antigen in urine using the IMMY ALPHA Histoplasma EIA kit (IAHE), clarus Histoplasma GM Enzyme Immunoassay (cHGEI IMMY) and MiraVista Histoplasma Urine Antigen LFA (MVHUALFA); as well as the Hcp100 and 1281-1283220SCAR nested PCRs in blood, bone-marrow, tissue biopsies and urine. Methodology/principal findings We included 415 PLWHIV older than 18 years of age with suspicion of PDH. Using as diagnostic standard recovery of H. capsulatum in blood, bone marrow or tissue cultures, or histopathological exam compatible, detected 108 patients (26%, [95%CI, 21.78-30.22]) with proven-PDH. We analyzed 391 urine samples by the IAHE, cHGEI IMMY and MVHUALFA; the sensitivity/specificity values obtained were 67.3% (95% CI, 57.4-76.2) / 96.2% (95% CI, 93.2-98.0) for IAHE, 91.3% (95% CI, 84.2-96.0) / 90.9% (95% CI, 87.0-94.0) for cHGEI IMMY and 90.4% (95% CI, 83.0-95.3) / 92.3% (95% CI, 88.6-95.1) for MVHUALFA. The Hcp100 nested PCR was performed on 393, 343, 75 and 297, blood, bone marrow, tissue and urine samples respectively; the sensitivity/specificity values obtained were 62.9% (95%CI, 53.3-72.5)/ 89.5% (95%CI, 86.0-93.0), 65.9% (95%CI, 56.0-75.8)/ 89.0% (95%CI, 85.2-92.9), 62.1% (95%CI, 44.4-79.7)/ 82.6% (95%CI, 71.7-93.6) and 34.9% (95%CI, 24.8-46.2)/ 67.3% (95%CI, 60.6-73.5) respectively; and 1281-1283220SCAR nested PCR was performed on 392, 344, 75 and 291, respectively; the sensitivity/specificity values obtained were 65.3% (95% CI, 55.9-74.7)/ 58.8% (95%CI, 53.2-64.5), 70.8% (95%CI, 61.3-80.2)/ 52.9% (95%CI, 46.8-59.1), 71.4% (95%CI, 54.7-88.2)/ 40.4% (95%CI, 26.4-54.5) and 18.1% (95%CI, 10.5-28.1)/ 90.4% (95%CI, 85.5-94.0), respectively. Conclusions/significance The cHGEI ... |
format |
Article in Journal/Newspaper |
author |
Areli Martínez-Gamboa María Dolores Niembro-Ortega Pedro Torres-González Janeth Santiago-Cruz Nancy Guadalupe Velázquez-Zavala Andrea Rangel-Cordero Brenda Crabtree-Ramírez Armando Gamboa-Domínguez Edgardo Reyes-Gutiérrez Gustavo Reyes-Terán Víctor Hugo Lozano-Fernandez Víctor Hugo Ahumada-Topete Pedro Martínez-Ayala Marisol Manríquez-Reyes Juan Pablo Ramírez-Hinojosa Patricia Rodríguez-Zulueta Christian Hernández-León Jesús Ruíz-Quiñones Norma Eréndira Rivera-Martínez Alberto Chaparro-Sánchez Jaime Andrade-Villanueva Luz Alicia González-Hernández Sofia Cruz-Martínez Oscar Flores-Barrientos Jesús Enrique Gaytán-Martínez Martín Magaña-Aquino Axel Cervantes-Sánchez Antonio Olivas-Martínez Javier Araujo-Meléndez María Del Rocío Reyes-Montes Esperanza Duarte-Escalante María Guadalupe Frías-De León José Antonio Ramírez María Lucia Taylor Alfredo Ponce de León-Garduño José Sifuentes-Osornio |
author_facet |
Areli Martínez-Gamboa María Dolores Niembro-Ortega Pedro Torres-González Janeth Santiago-Cruz Nancy Guadalupe Velázquez-Zavala Andrea Rangel-Cordero Brenda Crabtree-Ramírez Armando Gamboa-Domínguez Edgardo Reyes-Gutiérrez Gustavo Reyes-Terán Víctor Hugo Lozano-Fernandez Víctor Hugo Ahumada-Topete Pedro Martínez-Ayala Marisol Manríquez-Reyes Juan Pablo Ramírez-Hinojosa Patricia Rodríguez-Zulueta Christian Hernández-León Jesús Ruíz-Quiñones Norma Eréndira Rivera-Martínez Alberto Chaparro-Sánchez Jaime Andrade-Villanueva Luz Alicia González-Hernández Sofia Cruz-Martínez Oscar Flores-Barrientos Jesús Enrique Gaytán-Martínez Martín Magaña-Aquino Axel Cervantes-Sánchez Antonio Olivas-Martínez Javier Araujo-Meléndez María Del Rocío Reyes-Montes Esperanza Duarte-Escalante María Guadalupe Frías-De León José Antonio Ramírez María Lucia Taylor Alfredo Ponce de León-Garduño José Sifuentes-Osornio |
author_sort |
Areli Martínez-Gamboa |
title |
Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico. |
title_short |
Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico. |
title_full |
Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico. |
title_fullStr |
Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico. |
title_full_unstemmed |
Diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with HIV/AIDS: A prospective multicenter study in Mexico. |
title_sort |
diagnostic accuracy of antigen detection in urine and molecular assays testing in different clinical samples for the diagnosis of progressive disseminated histoplasmosis in patients living with hiv/aids: a prospective multicenter study in mexico. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009215 https://doaj.org/article/b93579871f1a49d1a1f7166df3e2a1d2 |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Arctic Eia |
geographic_facet |
Arctic Eia |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 3, p e0009215 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009215 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009215 https://doaj.org/article/b93579871f1a49d1a1f7166df3e2a1d2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009215 |
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PLOS Neglected Tropical Diseases |
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15 |
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3 |
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e0009215 |
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1766348600624807936 |