Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.).
Lower jaw deformity (LJD) is a skeletal anomaly affecting farmed triploid Atlantic salmon (Salmo salar L.) which leads to considerable economic losses for industry and has animal welfare implications. The present study employed transcriptome analysis in parallel with real-time qPCR techniques to cha...
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ftdoajarticles:oai:doaj.org/article:b8cb0535191846b3bc5eb81803c9d7c5 2023-05-15T15:31:06+02:00 Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.). Gianluca Amoroso Tomer Ventura Jennifer M Cobcroft Mark B Adams Abigail Elizur Chris G Carter 2016-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0168454 https://doaj.org/article/b8cb0535191846b3bc5eb81803c9d7c5 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5158070?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0168454 https://doaj.org/article/b8cb0535191846b3bc5eb81803c9d7c5 PLoS ONE, Vol 11, Iss 12, p e0168454 (2016) Medicine R Science Q article 2016 ftdoajarticles https://doi.org/10.1371/journal.pone.0168454 2022-12-31T13:22:58Z Lower jaw deformity (LJD) is a skeletal anomaly affecting farmed triploid Atlantic salmon (Salmo salar L.) which leads to considerable economic losses for industry and has animal welfare implications. The present study employed transcriptome analysis in parallel with real-time qPCR techniques to characterise for the first time the LJD condition in triploid Atlantic salmon juveniles using two independent sample sets: experimentally-sourced salmon (60 g) and commercially produced salmon (100 g). A total of eleven genes, some detected/identified through the transcriptome analysis (fbn2, gal and gphb5) and others previously determined to be related to skeletal physiology (alp, bmp4, col1a1, col2a1, fgf23, igf1, mmp13, ocn), were tested in the two independent sample sets. Gphb5, a recently discovered hormone, was significantly (P < 0.05) down-regulated in LJD affected fish in both sample sets, suggesting a possible hormonal involvement. In-situ hybridization detected gphb5 expression in oral epithelium, teeth and skin of the lower jaw. Col2a1 showed the same consistent significant (P < 0.05) down-regulation in LJD suggesting a possible cartilaginous impairment as a distinctive feature of the condition. Significant (P < 0.05) differential expression of other genes found in either one or the other sample set highlighted the possible effect of stage of development or condition progression on transcription and showed that anomalous bone development, likely driven by cartilage impairment, is more evident at larger fish sizes. The present study improved our understanding of LJD suggesting that a cartilage impairment likely underlies the condition and col2a1 may be a marker. In addition, the involvement of gphb5 urges further investigation of a hormonal role in LJD and skeletal physiology in general. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles PLOS ONE 11 12 e0168454 |
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Medicine R Science Q Gianluca Amoroso Tomer Ventura Jennifer M Cobcroft Mark B Adams Abigail Elizur Chris G Carter Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.). |
topic_facet |
Medicine R Science Q |
description |
Lower jaw deformity (LJD) is a skeletal anomaly affecting farmed triploid Atlantic salmon (Salmo salar L.) which leads to considerable economic losses for industry and has animal welfare implications. The present study employed transcriptome analysis in parallel with real-time qPCR techniques to characterise for the first time the LJD condition in triploid Atlantic salmon juveniles using two independent sample sets: experimentally-sourced salmon (60 g) and commercially produced salmon (100 g). A total of eleven genes, some detected/identified through the transcriptome analysis (fbn2, gal and gphb5) and others previously determined to be related to skeletal physiology (alp, bmp4, col1a1, col2a1, fgf23, igf1, mmp13, ocn), were tested in the two independent sample sets. Gphb5, a recently discovered hormone, was significantly (P < 0.05) down-regulated in LJD affected fish in both sample sets, suggesting a possible hormonal involvement. In-situ hybridization detected gphb5 expression in oral epithelium, teeth and skin of the lower jaw. Col2a1 showed the same consistent significant (P < 0.05) down-regulation in LJD suggesting a possible cartilaginous impairment as a distinctive feature of the condition. Significant (P < 0.05) differential expression of other genes found in either one or the other sample set highlighted the possible effect of stage of development or condition progression on transcription and showed that anomalous bone development, likely driven by cartilage impairment, is more evident at larger fish sizes. The present study improved our understanding of LJD suggesting that a cartilage impairment likely underlies the condition and col2a1 may be a marker. In addition, the involvement of gphb5 urges further investigation of a hormonal role in LJD and skeletal physiology in general. |
format |
Article in Journal/Newspaper |
author |
Gianluca Amoroso Tomer Ventura Jennifer M Cobcroft Mark B Adams Abigail Elizur Chris G Carter |
author_facet |
Gianluca Amoroso Tomer Ventura Jennifer M Cobcroft Mark B Adams Abigail Elizur Chris G Carter |
author_sort |
Gianluca Amoroso |
title |
Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.). |
title_short |
Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.). |
title_full |
Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.). |
title_fullStr |
Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.). |
title_full_unstemmed |
Multigenic Delineation of Lower Jaw Deformity in Triploid Atlantic Salmon (Salmo salar L.). |
title_sort |
multigenic delineation of lower jaw deformity in triploid atlantic salmon (salmo salar l.). |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pone.0168454 https://doaj.org/article/b8cb0535191846b3bc5eb81803c9d7c5 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
PLoS ONE, Vol 11, Iss 12, p e0168454 (2016) |
op_relation |
http://europepmc.org/articles/PMC5158070?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0168454 https://doaj.org/article/b8cb0535191846b3bc5eb81803c9d7c5 |
op_doi |
https://doi.org/10.1371/journal.pone.0168454 |
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PLOS ONE |
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11 |
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12 |
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e0168454 |
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