Oxidative Stress and Digestive Enzyme Activity of Flatfish Larvae in a Changing Ocean.
Until now, it is not known how the antioxidant and digestive enzymatic machinery of fish early life stages will change with the combined effects of future ocean acidification and warming. Here we show that high pCO2 (~1600 μatm) significantly decreased metabolic rates (up to 27.4 %) of flatfish larv...
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ftdoajarticles:oai:doaj.org/article:b8c247fe9ef74aaf9db27ec29f5399d5 2023-05-15T17:50:46+02:00 Oxidative Stress and Digestive Enzyme Activity of Flatfish Larvae in a Changing Ocean. Marta S Pimentel Filipa Faleiro Mário Diniz Jorge Machado Pedro Pousão-Ferreira Myron A Peck Hans O Pörtner Rui Rosa 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0134082 https://doaj.org/article/b8c247fe9ef74aaf9db27ec29f5399d5 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4519323?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0134082 https://doaj.org/article/b8c247fe9ef74aaf9db27ec29f5399d5 PLoS ONE, Vol 10, Iss 7, p e0134082 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0134082 2022-12-31T14:10:37Z Until now, it is not known how the antioxidant and digestive enzymatic machinery of fish early life stages will change with the combined effects of future ocean acidification and warming. Here we show that high pCO2 (~1600 μatm) significantly decreased metabolic rates (up to 27.4 %) of flatfish larvae, Solea senegalensis, at both present (18 °C) and warmer temperatures (+4 °C). Moreover, both warming and hypercapnia increased the heat shock response and the activity of antioxidant enzymes, namely catalase (CAT) and glutathione S-transferase (GST), mainly in post-metamorphic larvae (30 dph). The lack of changes in the activity of CAT and GST of pre-metamorphic larvae (10 dph) seems to indicate that earlier stages lack a fully-developed antioxidant defense system. Nevertheless, the heat shock and antioxidant responses of post-metamorphic larvae were not enough to avoid the peroxidative damage, which was greatly increased under future environmental conditions. Digestive enzymatic activity of S. senegalensis larvae was also affected by future predictions. Hypercapnic conditions led to a decrease in the activity of digestive enzymes, both pancreatic (up to 26.1 % for trypsin and 74.5 % for amylase) and intestinal enzymes (up to 36.1 % for alkaline phosphatase) in post-metamorphic larvae. Moreover, the impact of ocean acidification and warming on some of these physiological and biochemical variables (namely, lower OCR and higher HSP and MDA levels) were translated into larvae performance, being significantly correlated with decreased larval growth and survival or increased incidence of skeletal deformities. The increased vulnerability of flatfish early life stages under future ocean conditions is expected to potentially determine recruitment and population dynamics in marine ecosystems. Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles PLOS ONE 10 7 e0134082 |
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Medicine R Science Q Marta S Pimentel Filipa Faleiro Mário Diniz Jorge Machado Pedro Pousão-Ferreira Myron A Peck Hans O Pörtner Rui Rosa Oxidative Stress and Digestive Enzyme Activity of Flatfish Larvae in a Changing Ocean. |
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Medicine R Science Q |
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Until now, it is not known how the antioxidant and digestive enzymatic machinery of fish early life stages will change with the combined effects of future ocean acidification and warming. Here we show that high pCO2 (~1600 μatm) significantly decreased metabolic rates (up to 27.4 %) of flatfish larvae, Solea senegalensis, at both present (18 °C) and warmer temperatures (+4 °C). Moreover, both warming and hypercapnia increased the heat shock response and the activity of antioxidant enzymes, namely catalase (CAT) and glutathione S-transferase (GST), mainly in post-metamorphic larvae (30 dph). The lack of changes in the activity of CAT and GST of pre-metamorphic larvae (10 dph) seems to indicate that earlier stages lack a fully-developed antioxidant defense system. Nevertheless, the heat shock and antioxidant responses of post-metamorphic larvae were not enough to avoid the peroxidative damage, which was greatly increased under future environmental conditions. Digestive enzymatic activity of S. senegalensis larvae was also affected by future predictions. Hypercapnic conditions led to a decrease in the activity of digestive enzymes, both pancreatic (up to 26.1 % for trypsin and 74.5 % for amylase) and intestinal enzymes (up to 36.1 % for alkaline phosphatase) in post-metamorphic larvae. Moreover, the impact of ocean acidification and warming on some of these physiological and biochemical variables (namely, lower OCR and higher HSP and MDA levels) were translated into larvae performance, being significantly correlated with decreased larval growth and survival or increased incidence of skeletal deformities. The increased vulnerability of flatfish early life stages under future ocean conditions is expected to potentially determine recruitment and population dynamics in marine ecosystems. |
format |
Article in Journal/Newspaper |
author |
Marta S Pimentel Filipa Faleiro Mário Diniz Jorge Machado Pedro Pousão-Ferreira Myron A Peck Hans O Pörtner Rui Rosa |
author_facet |
Marta S Pimentel Filipa Faleiro Mário Diniz Jorge Machado Pedro Pousão-Ferreira Myron A Peck Hans O Pörtner Rui Rosa |
author_sort |
Marta S Pimentel |
title |
Oxidative Stress and Digestive Enzyme Activity of Flatfish Larvae in a Changing Ocean. |
title_short |
Oxidative Stress and Digestive Enzyme Activity of Flatfish Larvae in a Changing Ocean. |
title_full |
Oxidative Stress and Digestive Enzyme Activity of Flatfish Larvae in a Changing Ocean. |
title_fullStr |
Oxidative Stress and Digestive Enzyme Activity of Flatfish Larvae in a Changing Ocean. |
title_full_unstemmed |
Oxidative Stress and Digestive Enzyme Activity of Flatfish Larvae in a Changing Ocean. |
title_sort |
oxidative stress and digestive enzyme activity of flatfish larvae in a changing ocean. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0134082 https://doaj.org/article/b8c247fe9ef74aaf9db27ec29f5399d5 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
PLoS ONE, Vol 10, Iss 7, p e0134082 (2015) |
op_relation |
http://europepmc.org/articles/PMC4519323?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0134082 https://doaj.org/article/b8c247fe9ef74aaf9db27ec29f5399d5 |
op_doi |
https://doi.org/10.1371/journal.pone.0134082 |
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PLOS ONE |
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10 |
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7 |
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e0134082 |
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