Enterotoxins, Biofilm and Resistance to Antimicrobial Agents of Coagulase-Negative Staphylococcus Strains Isolated from Blood Cultures
Coagulase-negative staphylococci (CNS) have been identified as the etiological agent in various infections and are currently the microorganisms most frequently isolated in blood cultures. However, little is known about the virulence factors produced by CNS that contribute to the pathogenesis of infe...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
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ftdoajarticles:oai:doaj.org/article:b8b20629b64c4bcf9312c526b73f3684 2023-10-01T03:54:09+02:00 Enterotoxins, Biofilm and Resistance to Antimicrobial Agents of Coagulase-Negative Staphylococcus Strains Isolated from Blood Cultures Karelis Lilibeth Nava Terán Dalialba María Sánchez Galvis Gladis Colina López Kutchynskaya Valero Leal 2013-10-01T00:00:00Z https://doaj.org/article/b8b20629b64c4bcf9312c526b73f3684 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://produccioncientificaluz.org/index.php/kasmera/article/view/8318 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/b8b20629b64c4bcf9312c526b73f3684 Kasmera, Vol 41, Iss 2, Pp 106-114 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles 2023-09-03T00:44:54Z Coagulase-negative staphylococci (CNS) have been identified as the etiological agent in various infections and are currently the microorganisms most frequently isolated in blood cultures. However, little is known about the virulence factors produced by CNS that contribute to the pathogenesis of infections caused by these microorganisms. This study was designed to determine the production of staphylococcal enterotoxins, biofilm production and resistance to antimicrobial agents. For this purpose, a total of 48 CNS strains isolated from blood cultures at the University Hospital in Maracaibo, Zulia, Venezuela, were studied. Staphylococcal enterotoxins were detected by reversed passive latex agglutination. Biofilm production was assessed in a quantitative assay using a microtiter-plate. Susceptibility against antimicrobial agents was tested by the disk diffusion method. Six strains of staphylococci isolates (12.5%) were found to be enterotoxigenic. Of these, 3 strains were positive for staphylococcal enterotoxin D (SED), 2 for SEA and 1 of the isolates was positive for SEB, SEC and SED.Twenty-one isolates (44%) were found to be biofilm producers. A high frequency of resistance was observed with oxacillin (90%), gentamicin (83%) and erythromycin (67%). None of the isolates showed resistance to vancomycin. Taking into consideration that the etiological importance of CNS has often been neglected, the present investigation confirmed that these microorganisms should not be ignored or classified as mere contaminants. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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English Spanish |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Karelis Lilibeth Nava Terán Dalialba María Sánchez Galvis Gladis Colina López Kutchynskaya Valero Leal Enterotoxins, Biofilm and Resistance to Antimicrobial Agents of Coagulase-Negative Staphylococcus Strains Isolated from Blood Cultures |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Coagulase-negative staphylococci (CNS) have been identified as the etiological agent in various infections and are currently the microorganisms most frequently isolated in blood cultures. However, little is known about the virulence factors produced by CNS that contribute to the pathogenesis of infections caused by these microorganisms. This study was designed to determine the production of staphylococcal enterotoxins, biofilm production and resistance to antimicrobial agents. For this purpose, a total of 48 CNS strains isolated from blood cultures at the University Hospital in Maracaibo, Zulia, Venezuela, were studied. Staphylococcal enterotoxins were detected by reversed passive latex agglutination. Biofilm production was assessed in a quantitative assay using a microtiter-plate. Susceptibility against antimicrobial agents was tested by the disk diffusion method. Six strains of staphylococci isolates (12.5%) were found to be enterotoxigenic. Of these, 3 strains were positive for staphylococcal enterotoxin D (SED), 2 for SEA and 1 of the isolates was positive for SEB, SEC and SED.Twenty-one isolates (44%) were found to be biofilm producers. A high frequency of resistance was observed with oxacillin (90%), gentamicin (83%) and erythromycin (67%). None of the isolates showed resistance to vancomycin. Taking into consideration that the etiological importance of CNS has often been neglected, the present investigation confirmed that these microorganisms should not be ignored or classified as mere contaminants. |
format |
Article in Journal/Newspaper |
author |
Karelis Lilibeth Nava Terán Dalialba María Sánchez Galvis Gladis Colina López Kutchynskaya Valero Leal |
author_facet |
Karelis Lilibeth Nava Terán Dalialba María Sánchez Galvis Gladis Colina López Kutchynskaya Valero Leal |
author_sort |
Karelis Lilibeth Nava Terán |
title |
Enterotoxins, Biofilm and Resistance to Antimicrobial Agents of Coagulase-Negative Staphylococcus Strains Isolated from Blood Cultures |
title_short |
Enterotoxins, Biofilm and Resistance to Antimicrobial Agents of Coagulase-Negative Staphylococcus Strains Isolated from Blood Cultures |
title_full |
Enterotoxins, Biofilm and Resistance to Antimicrobial Agents of Coagulase-Negative Staphylococcus Strains Isolated from Blood Cultures |
title_fullStr |
Enterotoxins, Biofilm and Resistance to Antimicrobial Agents of Coagulase-Negative Staphylococcus Strains Isolated from Blood Cultures |
title_full_unstemmed |
Enterotoxins, Biofilm and Resistance to Antimicrobial Agents of Coagulase-Negative Staphylococcus Strains Isolated from Blood Cultures |
title_sort |
enterotoxins, biofilm and resistance to antimicrobial agents of coagulase-negative staphylococcus strains isolated from blood cultures |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2013 |
url |
https://doaj.org/article/b8b20629b64c4bcf9312c526b73f3684 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 41, Iss 2, Pp 106-114 (2013) |
op_relation |
https://produccioncientificaluz.org/index.php/kasmera/article/view/8318 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/b8b20629b64c4bcf9312c526b73f3684 |
_version_ |
1778521529748815872 |