Jeju seaweeds suppress lipopolysaccharide-stimulated proinflammatory response in RAW 264.7 murine macrophages
Objective: To investigate the anti-inflammatory effects of Jeju seaweeds on macrophage RAW 264.7 cells under lipopolysaccharide (LPS) stimulation. Methods: Ethyl acetate fractions were prepared from five different types of Jeju seaweeds, Dictyopteris divaricata (D. divaricata), Dictyopteris prolifer...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Language: | English |
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Wolters Kluwer Medknow Publications
2014
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Online Access: | https://doi.org/10.12980/APJTB.4.2014C1099 https://doaj.org/article/b8973e9de2ba475d8529c3a4a71697b1 |
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ftdoajarticles:oai:doaj.org/article:b8973e9de2ba475d8529c3a4a71697b1 2023-05-15T15:15:02+02:00 Jeju seaweeds suppress lipopolysaccharide-stimulated proinflammatory response in RAW 264.7 murine macrophages Eun-Jin Yang Ji-Young Moon Sang Suk Kim Kyong-Wol Yang Wook Jae Lee Nam Ho Lee Chang-Gu Hyun 2014-07-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014C1099 https://doaj.org/article/b8973e9de2ba475d8529c3a4a71697b1 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115301246 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1099 https://doaj.org/article/b8973e9de2ba475d8529c3a4a71697b1 Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 7, Pp 529-537 (2014) Nitric oxide Interleukin-6 Prostaglandin E2 Tumor necrosis factor-α Seaweeds Proinflammatory mediators Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014C1099 2022-12-31T00:44:38Z Objective: To investigate the anti-inflammatory effects of Jeju seaweeds on macrophage RAW 264.7 cells under lipopolysaccharide (LPS) stimulation. Methods: Ethyl acetate fractions were prepared from five different types of Jeju seaweeds, Dictyopteris divaricata (D. divaricata), Dictyopteris prolifera (D. prolifera), Prionitis cornea (P. cornea), Grateloupia lanceolata (G. lanceolata), and Grateloupia filicina (G. filicina). They were screened for inhibitory effects on proinflammatory mediators and cytokines such as nitric oxide (NO), prostaglandin E2, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6). Results: Our results revealed that D. divaricata, D. prolifera, P. cornea, G. lanceolata, and G. filicina potently inhibited LPS-stimulated NO production (IC50 values were 18.0, 38.36, 38.43, 32.81 and 37.14 μg/mL, respectively). Consistent with these findings, D. divaricata, D. prolifera, P. cornea, and G. filicina also reduced the LPS-induced and prostaglandin E2 production in a concentration-dependent manner. Expectedly, they suppressed the expression of inducible NO synthase and cyclooxygenase-2 at the protein level in a dose-dependent manner in the RAW 264.7 cells, as determined by western blotting. In addition, the levels of TNF-α and IL-6, released into the medium, were also reduced by D. divaricata, D. prolifera, P. cornea, G. lanceolata, and G. filicina in a dose-dependent manner (IC50 values for TNF-α were 16.11, 28.21, 84.27, 45.52 and 74.75 μg/mL, respectively; IC50 values for IL-6 were 37.35, 80.08, 103.28, 62.53 and 84.28 μg/mL, respectively). The total phlorotannin content was measured by the Folin-Ciocalteu method and expressed as phloroglucinol equivalents. The content was 92.0 μg/mg for D. divaricata, 151.8 μg/mg for D. prolifera, 57.2 μg/mg for P. cornea, 53.0 μg/mg for G. lanceolata, and 40.2 μg/mg for G. filicina. Conclusions: Thus, these findings suggest that Jeju seaweed extracts have potential therapeutic applications for inflammatory responses. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 7 529 537 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Nitric oxide Interleukin-6 Prostaglandin E2 Tumor necrosis factor-α Seaweeds Proinflammatory mediators Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Nitric oxide Interleukin-6 Prostaglandin E2 Tumor necrosis factor-α Seaweeds Proinflammatory mediators Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Eun-Jin Yang Ji-Young Moon Sang Suk Kim Kyong-Wol Yang Wook Jae Lee Nam Ho Lee Chang-Gu Hyun Jeju seaweeds suppress lipopolysaccharide-stimulated proinflammatory response in RAW 264.7 murine macrophages |
topic_facet |
Nitric oxide Interleukin-6 Prostaglandin E2 Tumor necrosis factor-α Seaweeds Proinflammatory mediators Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the anti-inflammatory effects of Jeju seaweeds on macrophage RAW 264.7 cells under lipopolysaccharide (LPS) stimulation. Methods: Ethyl acetate fractions were prepared from five different types of Jeju seaweeds, Dictyopteris divaricata (D. divaricata), Dictyopteris prolifera (D. prolifera), Prionitis cornea (P. cornea), Grateloupia lanceolata (G. lanceolata), and Grateloupia filicina (G. filicina). They were screened for inhibitory effects on proinflammatory mediators and cytokines such as nitric oxide (NO), prostaglandin E2, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6). Results: Our results revealed that D. divaricata, D. prolifera, P. cornea, G. lanceolata, and G. filicina potently inhibited LPS-stimulated NO production (IC50 values were 18.0, 38.36, 38.43, 32.81 and 37.14 μg/mL, respectively). Consistent with these findings, D. divaricata, D. prolifera, P. cornea, and G. filicina also reduced the LPS-induced and prostaglandin E2 production in a concentration-dependent manner. Expectedly, they suppressed the expression of inducible NO synthase and cyclooxygenase-2 at the protein level in a dose-dependent manner in the RAW 264.7 cells, as determined by western blotting. In addition, the levels of TNF-α and IL-6, released into the medium, were also reduced by D. divaricata, D. prolifera, P. cornea, G. lanceolata, and G. filicina in a dose-dependent manner (IC50 values for TNF-α were 16.11, 28.21, 84.27, 45.52 and 74.75 μg/mL, respectively; IC50 values for IL-6 were 37.35, 80.08, 103.28, 62.53 and 84.28 μg/mL, respectively). The total phlorotannin content was measured by the Folin-Ciocalteu method and expressed as phloroglucinol equivalents. The content was 92.0 μg/mg for D. divaricata, 151.8 μg/mg for D. prolifera, 57.2 μg/mg for P. cornea, 53.0 μg/mg for G. lanceolata, and 40.2 μg/mg for G. filicina. Conclusions: Thus, these findings suggest that Jeju seaweed extracts have potential therapeutic applications for inflammatory responses. |
format |
Article in Journal/Newspaper |
author |
Eun-Jin Yang Ji-Young Moon Sang Suk Kim Kyong-Wol Yang Wook Jae Lee Nam Ho Lee Chang-Gu Hyun |
author_facet |
Eun-Jin Yang Ji-Young Moon Sang Suk Kim Kyong-Wol Yang Wook Jae Lee Nam Ho Lee Chang-Gu Hyun |
author_sort |
Eun-Jin Yang |
title |
Jeju seaweeds suppress lipopolysaccharide-stimulated proinflammatory response in RAW 264.7 murine macrophages |
title_short |
Jeju seaweeds suppress lipopolysaccharide-stimulated proinflammatory response in RAW 264.7 murine macrophages |
title_full |
Jeju seaweeds suppress lipopolysaccharide-stimulated proinflammatory response in RAW 264.7 murine macrophages |
title_fullStr |
Jeju seaweeds suppress lipopolysaccharide-stimulated proinflammatory response in RAW 264.7 murine macrophages |
title_full_unstemmed |
Jeju seaweeds suppress lipopolysaccharide-stimulated proinflammatory response in RAW 264.7 murine macrophages |
title_sort |
jeju seaweeds suppress lipopolysaccharide-stimulated proinflammatory response in raw 264.7 murine macrophages |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014C1099 https://doaj.org/article/b8973e9de2ba475d8529c3a4a71697b1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 7, Pp 529-537 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115301246 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1099 https://doaj.org/article/b8973e9de2ba475d8529c3a4a71697b1 |
op_doi |
https://doi.org/10.12980/APJTB.4.2014C1099 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
7 |
container_start_page |
529 |
op_container_end_page |
537 |
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1766345422441283584 |