Evaluación de la susceptibilidad antifúngica y cariotipo de Cryptococcus neoformans, aislados de muestras clínicas en Venezuela

• Main Authors: Calvo B, Colombo A, Fischman O, Santiago A, Nunes F, Branchini ML
• Format: Article in Journal/Newspaper
• Language: English; Spanish
• Published: Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales 2000
• Subjects: Susceptibilidad; cariotipo; Cryptococcus neoformans; Venezuela; Susceptibility; karyotype; Arctic medicine. Tropical medicine; RC955-962; Public aspects of medicine; RA1-1270; Arctic
• Online Access: https://doaj.org/article/b8967f9228b84fe7adf706a937fc786d

ABSTRACT: Thirty (30) samples coming from 27 HIV infected and non-infected patients were studied to assess antimycotic susceptibility and karyotype of Cryptococcus neoformans clinical isolates obtained in Venezuela. Identification of each sample was performed by standard methods, variety classification was based on growth in CGB agar medium, serotyping was performed by slide agglutination with five specific sera. Antifungal susceptibility was performed for amphotericin B, fluconazole, itraconazole and 5-flucytosine, according to NCCLS recommendations and karyotype study was done through contour clamped homogenous field gel electrophoresis (CHEF). Twenty-seven (27) isolates of C. neoformans var. neoformans were obtained, all being of serotype A, and three (3) isolates of C. neoformans var. gattii, two (2) belonging to serotype B and one (1) to serotype C. Strains of this series were highly susceptible to the antifungal agents tested. Electrophoretic Karyotype (EK) analysis showed 13 profiles, presenting between 7 and 9 chromosomes and the molecular weight variation was 450/565 and 2200 kb. No significant differences were observed between the minimum inhibitory concentrations (MIC), nor was it possible to associate EK profiles among the isolates of HIV and non-HIV patients. Further clinical and laboratorial investigations are mandatory to improve the understanding of C. neoformans in Venezuela. RESUMEN: Para evaluar la susceptibilidad a los antimicóticos y el cariotipo de aislados clínicos de Cryptococcus neoformans obtenidos en Venezuela, fueron estudiadas 30 muestras, procedentes de 27 pacientes con y sin infección por VIH. A cada una se le hizo identificación por métodos clásicos, estudio de la variedad a través del medio CGB, serotipifícación por aglutinación con 5 antisueros, pruebas de susceptibilidad a anfotericina B, fluconazol, itraconzaol y 5-fluorocitosina, según la metodología del NCCLS y estudio del cariotipo por electroforesis de campo pulsado usando el sistema "contour-clamped homogeneous field gel ...