Evaluación de la susceptibilidad antifúngica y cariotipo de Cryptococcus neoformans, aislados de muestras clínicas en Venezuela
ABSTRACT: Thirty (30) samples coming from 27 HIV infected and non-infected patients were studied to assess antimycotic susceptibility and karyotype of Cryptococcus neoformans clinical isolates obtained in Venezuela. Identification of each sample was performed by standard methods, variety classificat...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2000
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ftdoajarticles:oai:doaj.org/article:b8967f9228b84fe7adf706a937fc786d 2023-10-01T03:54:21+02:00 Evaluación de la susceptibilidad antifúngica y cariotipo de Cryptococcus neoformans, aislados de muestras clínicas en Venezuela Calvo B Colombo A Fischman O Santiago A Nunes F Branchini ML 2000-12-01T00:00:00Z https://doaj.org/article/b8967f9228b84fe7adf706a937fc786d EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://drive.google.com/file/d/13SZZXWHfB_Qdi8Am-h3bf6Q-3w2LIcEF/view https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/b8967f9228b84fe7adf706a937fc786d Kasmera, Vol 28, Iss 3 (2000) Susceptibilidad cariotipo Cryptococcus neoformans Venezuela Susceptibility karyotype Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2000 ftdoajarticles 2023-09-03T00:41:34Z ABSTRACT: Thirty (30) samples coming from 27 HIV infected and non-infected patients were studied to assess antimycotic susceptibility and karyotype of Cryptococcus neoformans clinical isolates obtained in Venezuela. Identification of each sample was performed by standard methods, variety classification was based on growth in CGB agar medium, serotyping was performed by slide agglutination with five specific sera. Antifungal susceptibility was performed for amphotericin B, fluconazole, itraconazole and 5-flucytosine, according to NCCLS recommendations and karyotype study was done through contour clamped homogenous field gel electrophoresis (CHEF). Twenty-seven (27) isolates of C. neoformans var. neoformans were obtained, all being of serotype A, and three (3) isolates of C. neoformans var. gattii, two (2) belonging to serotype B and one (1) to serotype C. Strains of this series were highly susceptible to the antifungal agents tested. Electrophoretic Karyotype (EK) analysis showed 13 profiles, presenting between 7 and 9 chromosomes and the molecular weight variation was 450/565 and 2200 kb. No significant differences were observed between the minimum inhibitory concentrations (MIC), nor was it possible to associate EK profiles among the isolates of HIV and non-HIV patients. Further clinical and laboratorial investigations are mandatory to improve the understanding of C. neoformans in Venezuela. RESUMEN: Para evaluar la susceptibilidad a los antimicóticos y el cariotipo de aislados clínicos de Cryptococcus neoformans obtenidos en Venezuela, fueron estudiadas 30 muestras, procedentes de 27 pacientes con y sin infección por VIH. A cada una se le hizo identificación por métodos clásicos, estudio de la variedad a través del medio CGB, serotipifícación por aglutinación con 5 antisueros, pruebas de susceptibilidad a anfotericina B, fluconazol, itraconzaol y 5-fluorocitosina, según la metodología del NCCLS y estudio del cariotipo por electroforesis de campo pulsado usando el sistema "contour-clamped homogeneous field gel ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English Spanish |
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Susceptibilidad cariotipo Cryptococcus neoformans Venezuela Susceptibility karyotype Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Susceptibilidad cariotipo Cryptococcus neoformans Venezuela Susceptibility karyotype Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Calvo B Colombo A Fischman O Santiago A Nunes F Branchini ML Evaluación de la susceptibilidad antifúngica y cariotipo de Cryptococcus neoformans, aislados de muestras clínicas en Venezuela |
topic_facet |
Susceptibilidad cariotipo Cryptococcus neoformans Venezuela Susceptibility karyotype Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
ABSTRACT: Thirty (30) samples coming from 27 HIV infected and non-infected patients were studied to assess antimycotic susceptibility and karyotype of Cryptococcus neoformans clinical isolates obtained in Venezuela. Identification of each sample was performed by standard methods, variety classification was based on growth in CGB agar medium, serotyping was performed by slide agglutination with five specific sera. Antifungal susceptibility was performed for amphotericin B, fluconazole, itraconazole and 5-flucytosine, according to NCCLS recommendations and karyotype study was done through contour clamped homogenous field gel electrophoresis (CHEF). Twenty-seven (27) isolates of C. neoformans var. neoformans were obtained, all being of serotype A, and three (3) isolates of C. neoformans var. gattii, two (2) belonging to serotype B and one (1) to serotype C. Strains of this series were highly susceptible to the antifungal agents tested. Electrophoretic Karyotype (EK) analysis showed 13 profiles, presenting between 7 and 9 chromosomes and the molecular weight variation was 450/565 and 2200 kb. No significant differences were observed between the minimum inhibitory concentrations (MIC), nor was it possible to associate EK profiles among the isolates of HIV and non-HIV patients. Further clinical and laboratorial investigations are mandatory to improve the understanding of C. neoformans in Venezuela. RESUMEN: Para evaluar la susceptibilidad a los antimicóticos y el cariotipo de aislados clínicos de Cryptococcus neoformans obtenidos en Venezuela, fueron estudiadas 30 muestras, procedentes de 27 pacientes con y sin infección por VIH. A cada una se le hizo identificación por métodos clásicos, estudio de la variedad a través del medio CGB, serotipifícación por aglutinación con 5 antisueros, pruebas de susceptibilidad a anfotericina B, fluconazol, itraconzaol y 5-fluorocitosina, según la metodología del NCCLS y estudio del cariotipo por electroforesis de campo pulsado usando el sistema "contour-clamped homogeneous field gel ... |
format |
Article in Journal/Newspaper |
author |
Calvo B Colombo A Fischman O Santiago A Nunes F Branchini ML |
author_facet |
Calvo B Colombo A Fischman O Santiago A Nunes F Branchini ML |
author_sort |
Calvo B |
title |
Evaluación de la susceptibilidad antifúngica y cariotipo de Cryptococcus neoformans, aislados de muestras clínicas en Venezuela |
title_short |
Evaluación de la susceptibilidad antifúngica y cariotipo de Cryptococcus neoformans, aislados de muestras clínicas en Venezuela |
title_full |
Evaluación de la susceptibilidad antifúngica y cariotipo de Cryptococcus neoformans, aislados de muestras clínicas en Venezuela |
title_fullStr |
Evaluación de la susceptibilidad antifúngica y cariotipo de Cryptococcus neoformans, aislados de muestras clínicas en Venezuela |
title_full_unstemmed |
Evaluación de la susceptibilidad antifúngica y cariotipo de Cryptococcus neoformans, aislados de muestras clínicas en Venezuela |
title_sort |
evaluación de la susceptibilidad antifúngica y cariotipo de cryptococcus neoformans, aislados de muestras clínicas en venezuela |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2000 |
url |
https://doaj.org/article/b8967f9228b84fe7adf706a937fc786d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 28, Iss 3 (2000) |
op_relation |
https://drive.google.com/file/d/13SZZXWHfB_Qdi8Am-h3bf6Q-3w2LIcEF/view https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/b8967f9228b84fe7adf706a937fc786d |
_version_ |
1778521856242876416 |