Epimastigogénesis de Trypanosoma cruzi: método para estudiar in vivo la transformación de tripomastigota en epimastigota
Abstract: Trypanosoma cruzi epimastigogenesis naturally occurs in the intestine of the invertebrate host. It is not known whether the morphological changes that occur during transformation of bloodstream trypomastigotes to epimastigotes are identical for different T. cruzi isolates. This research sh...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2012
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ftdoajarticles:oai:doaj.org/article:b881bce619964db8bdb5e5a9e3c6f91f 2023-10-01T03:54:19+02:00 Epimastigogénesis de Trypanosoma cruzi: método para estudiar in vivo la transformación de tripomastigota en epimastigota Navarro A María C Córdova L Angi S Fernández B Karla N Arteaga O Rosa Y Graterol R Diana I Domínguez B María I De Lima R Ana R Pineda Wilmer A Contreras A Víctor T 2012-12-01T00:00:00Z https://doaj.org/article/b881bce619964db8bdb5e5a9e3c6f91f EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://sites.google.com/prod/view/revistakasmera/vol%C3%BAmenes/2011-2015-vols-39-43/2012-v-40-n-2/122-133?authuser=0 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/b881bce619964db8bdb5e5a9e3c6f91f Kasmera, Vol 40, Iss 2, Pp 122-133 (2012) Trypanosoma cruzi epimastigogénesis in vitro e in vivo Rhodnius prolixus in vitro e in vivo epimastigogenesis Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles 2023-09-03T00:47:51Z Abstract: Trypanosoma cruzi epimastigogenesis naturally occurs in the intestine of the invertebrate host. It is not known whether the morphological changes that occur during transformation of bloodstream trypomastigotes to epimastigotes are identical for different T. cruzi isolates. This research shows a useful method for studying the events that occur during epimastigogenesis, comparing parasites from epidemiological sources. Rhodnius prolixus V stage nymphs were fed artificially with an ad hoc solution containing blood-like trypomastigotes and the morphological changes were examined during eight days. Anterior intestinal contents were removed by decapitation and squeezing the abdomen of the nymphs, which permitted obtaining quickly almost 100% of the inoculate with loads between 1.9 and 8.9 × 106 trypomastigotes/nymph. The number of parasites per nymph and morphological changes were determined using phase microscopy with Giemsa staining. The nymphs ingested 7.4 times their weight of the infecting solution with volumes between 101 e 357 µL (229 ± 66 mg); 50% of the ingest was eliminated as urine during the first 24 hours. The trypomastigotes transformed to rounded forms before evolving into epimastigotes following different kinetics according to the isolate. This method is proposed for rapid, quantitative study of the early events of epimastigogenesis for T. cruzi in vivo. Resumen: La epimastigogénesis de Trypanosoma cruzi ocurre naturalmente en el intestino del hospedador invertebrado. Se desconoce si los cambios morfológicos que ocurren durante la transformación de los tripomastigotas sanguíneos en epimastigotas son idénticos para diferentes aislados de T. cruzi. Aquí mostramos un método útil para estudiar los eventos que ocurren durante la epimastigogénesis, comparando parásitos de diferentes procedencias epidemiológicas. Se alimentaron artificialmente ninfas de V estadio de Rhodnius prolixus con una solución ad hoc conteniendo tripomastigotas tipo-sanguíneo y siguiendo los cambios morfológicos por 8 días. ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Estadio ENVELOPE(-54.700,-54.700,-61.117,-61.117) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
Trypanosoma cruzi epimastigogénesis in vitro e in vivo Rhodnius prolixus in vitro e in vivo epimastigogenesis Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Trypanosoma cruzi epimastigogénesis in vitro e in vivo Rhodnius prolixus in vitro e in vivo epimastigogenesis Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Navarro A María C Córdova L Angi S Fernández B Karla N Arteaga O Rosa Y Graterol R Diana I Domínguez B María I De Lima R Ana R Pineda Wilmer A Contreras A Víctor T Epimastigogénesis de Trypanosoma cruzi: método para estudiar in vivo la transformación de tripomastigota en epimastigota |
topic_facet |
Trypanosoma cruzi epimastigogénesis in vitro e in vivo Rhodnius prolixus in vitro e in vivo epimastigogenesis Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Abstract: Trypanosoma cruzi epimastigogenesis naturally occurs in the intestine of the invertebrate host. It is not known whether the morphological changes that occur during transformation of bloodstream trypomastigotes to epimastigotes are identical for different T. cruzi isolates. This research shows a useful method for studying the events that occur during epimastigogenesis, comparing parasites from epidemiological sources. Rhodnius prolixus V stage nymphs were fed artificially with an ad hoc solution containing blood-like trypomastigotes and the morphological changes were examined during eight days. Anterior intestinal contents were removed by decapitation and squeezing the abdomen of the nymphs, which permitted obtaining quickly almost 100% of the inoculate with loads between 1.9 and 8.9 × 106 trypomastigotes/nymph. The number of parasites per nymph and morphological changes were determined using phase microscopy with Giemsa staining. The nymphs ingested 7.4 times their weight of the infecting solution with volumes between 101 e 357 µL (229 ± 66 mg); 50% of the ingest was eliminated as urine during the first 24 hours. The trypomastigotes transformed to rounded forms before evolving into epimastigotes following different kinetics according to the isolate. This method is proposed for rapid, quantitative study of the early events of epimastigogenesis for T. cruzi in vivo. Resumen: La epimastigogénesis de Trypanosoma cruzi ocurre naturalmente en el intestino del hospedador invertebrado. Se desconoce si los cambios morfológicos que ocurren durante la transformación de los tripomastigotas sanguíneos en epimastigotas son idénticos para diferentes aislados de T. cruzi. Aquí mostramos un método útil para estudiar los eventos que ocurren durante la epimastigogénesis, comparando parásitos de diferentes procedencias epidemiológicas. Se alimentaron artificialmente ninfas de V estadio de Rhodnius prolixus con una solución ad hoc conteniendo tripomastigotas tipo-sanguíneo y siguiendo los cambios morfológicos por 8 días. ... |
format |
Article in Journal/Newspaper |
author |
Navarro A María C Córdova L Angi S Fernández B Karla N Arteaga O Rosa Y Graterol R Diana I Domínguez B María I De Lima R Ana R Pineda Wilmer A Contreras A Víctor T |
author_facet |
Navarro A María C Córdova L Angi S Fernández B Karla N Arteaga O Rosa Y Graterol R Diana I Domínguez B María I De Lima R Ana R Pineda Wilmer A Contreras A Víctor T |
author_sort |
Navarro A María C |
title |
Epimastigogénesis de Trypanosoma cruzi: método para estudiar in vivo la transformación de tripomastigota en epimastigota |
title_short |
Epimastigogénesis de Trypanosoma cruzi: método para estudiar in vivo la transformación de tripomastigota en epimastigota |
title_full |
Epimastigogénesis de Trypanosoma cruzi: método para estudiar in vivo la transformación de tripomastigota en epimastigota |
title_fullStr |
Epimastigogénesis de Trypanosoma cruzi: método para estudiar in vivo la transformación de tripomastigota en epimastigota |
title_full_unstemmed |
Epimastigogénesis de Trypanosoma cruzi: método para estudiar in vivo la transformación de tripomastigota en epimastigota |
title_sort |
epimastigogénesis de trypanosoma cruzi: método para estudiar in vivo la transformación de tripomastigota en epimastigota |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2012 |
url |
https://doaj.org/article/b881bce619964db8bdb5e5a9e3c6f91f |
long_lat |
ENVELOPE(-54.700,-54.700,-61.117,-61.117) |
geographic |
Arctic Estadio |
geographic_facet |
Arctic Estadio |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 40, Iss 2, Pp 122-133 (2012) |
op_relation |
https://sites.google.com/prod/view/revistakasmera/vol%C3%BAmenes/2011-2015-vols-39-43/2012-v-40-n-2/122-133?authuser=0 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/b881bce619964db8bdb5e5a9e3c6f91f |
_version_ |
1778521800952512512 |