Polymerase chain reaction in detecting Leishmania sp in symptomatic and asymptomatic seropositive dogs
In human and canine renal histological studies of visceral leishmaniasis (VL), the etiological agent is rarely found in situ. The objective of this study was to evaluate PCR in identifying the etiological agent in spleen, liver, lymph node, and kidneys of VL-seropositive dogs. Twenty-five symptomati...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
SciELO
2005
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S1678-91992005000400011 https://doaj.org/article/b8781c1c059548d581606c71cd1c1bce |
id |
ftdoajarticles:oai:doaj.org/article:b8781c1c059548d581606c71cd1c1bce |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:b8781c1c059548d581606c71cd1c1bce 2023-05-15T15:09:42+02:00 Polymerase chain reaction in detecting Leishmania sp in symptomatic and asymptomatic seropositive dogs M. J. V. Soares J. R. E. Moraes A. M. F. Roselino 2005-12-01T00:00:00Z https://doi.org/10.1590/S1678-91992005000400011 https://doaj.org/article/b8781c1c059548d581606c71cd1c1bce EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400011 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992005000400011 1678-9199 https://doaj.org/article/b8781c1c059548d581606c71cd1c1bce Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 11, Iss 4, Pp 532-539 (2005) dogs Leishmania PCR kidney Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2005 ftdoajarticles https://doi.org/10.1590/S1678-91992005000400011 2022-12-30T23:55:52Z In human and canine renal histological studies of visceral leishmaniasis (VL), the etiological agent is rarely found in situ. The objective of this study was to evaluate PCR in identifying the etiological agent in spleen, liver, lymph node, and kidneys of VL-seropositive dogs. Twenty-five symptomatic (case group) and 15 asymptomatic (control group) VL-seropositive dogs of different breeds, sexes, and ages from Teresina, Piauí State, Brazil, were used. Serologic diagnosis was made by enzyme-linked immunosorbent assay and indirect immunofluorescence test. Animals were subjected to euthanasia and necropsy. Renal fragments were immersed in buffered formaldehyde solution. Spleen, liver, lymph node, and kidney samples were collected and frozen at -70ºC until DNA extraction. After dehydration and diaphanization, renal fragments were infiltrated and embedded in paraffin, cut at 3 µm, and stained with hematoxylin-eosin (HE). DNA amplification used an automatic thermocycler with specific Leishmania primers. All case-group dogs and 2 controls showed positive results in spleen, liver, or lymph node PCRs. There was a significant difference by Fisher exact test. In symptomatic seropositive dogs, renal histopathological evaluation showed one animal (4%) with amastigote forms of Leishmania in inflammatory infiltrate, and kidney PCRs detected Leishmania DNA in eight animals (32%). The conclusion was that PCR is more precise than the conventional histopathology in detecting the Leishmania parasite in kidney. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 11 4 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
dogs Leishmania PCR kidney Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
dogs Leishmania PCR kidney Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 M. J. V. Soares J. R. E. Moraes A. M. F. Roselino Polymerase chain reaction in detecting Leishmania sp in symptomatic and asymptomatic seropositive dogs |
topic_facet |
dogs Leishmania PCR kidney Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
In human and canine renal histological studies of visceral leishmaniasis (VL), the etiological agent is rarely found in situ. The objective of this study was to evaluate PCR in identifying the etiological agent in spleen, liver, lymph node, and kidneys of VL-seropositive dogs. Twenty-five symptomatic (case group) and 15 asymptomatic (control group) VL-seropositive dogs of different breeds, sexes, and ages from Teresina, Piauí State, Brazil, were used. Serologic diagnosis was made by enzyme-linked immunosorbent assay and indirect immunofluorescence test. Animals were subjected to euthanasia and necropsy. Renal fragments were immersed in buffered formaldehyde solution. Spleen, liver, lymph node, and kidney samples were collected and frozen at -70ºC until DNA extraction. After dehydration and diaphanization, renal fragments were infiltrated and embedded in paraffin, cut at 3 µm, and stained with hematoxylin-eosin (HE). DNA amplification used an automatic thermocycler with specific Leishmania primers. All case-group dogs and 2 controls showed positive results in spleen, liver, or lymph node PCRs. There was a significant difference by Fisher exact test. In symptomatic seropositive dogs, renal histopathological evaluation showed one animal (4%) with amastigote forms of Leishmania in inflammatory infiltrate, and kidney PCRs detected Leishmania DNA in eight animals (32%). The conclusion was that PCR is more precise than the conventional histopathology in detecting the Leishmania parasite in kidney. |
format |
Article in Journal/Newspaper |
author |
M. J. V. Soares J. R. E. Moraes A. M. F. Roselino |
author_facet |
M. J. V. Soares J. R. E. Moraes A. M. F. Roselino |
author_sort |
M. J. V. Soares |
title |
Polymerase chain reaction in detecting Leishmania sp in symptomatic and asymptomatic seropositive dogs |
title_short |
Polymerase chain reaction in detecting Leishmania sp in symptomatic and asymptomatic seropositive dogs |
title_full |
Polymerase chain reaction in detecting Leishmania sp in symptomatic and asymptomatic seropositive dogs |
title_fullStr |
Polymerase chain reaction in detecting Leishmania sp in symptomatic and asymptomatic seropositive dogs |
title_full_unstemmed |
Polymerase chain reaction in detecting Leishmania sp in symptomatic and asymptomatic seropositive dogs |
title_sort |
polymerase chain reaction in detecting leishmania sp in symptomatic and asymptomatic seropositive dogs |
publisher |
SciELO |
publishDate |
2005 |
url |
https://doi.org/10.1590/S1678-91992005000400011 https://doaj.org/article/b8781c1c059548d581606c71cd1c1bce |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 11, Iss 4, Pp 532-539 (2005) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400011 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992005000400011 1678-9199 https://doaj.org/article/b8781c1c059548d581606c71cd1c1bce |
op_doi |
https://doi.org/10.1590/S1678-91992005000400011 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
11 |
container_issue |
4 |
_version_ |
1766340839786676224 |