Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae)
Objective: To evaluate the antibacterial activity and neuroprotective capacity of the ethanolic and aqueous extracts of Tarenaya spinosa (T. spinosa) as well as to determine and quantify some of its polyphenols by high performance liquid chromatography with diode-array detection (HPLC-DAD). Methods:...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Language: | English |
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Wolters Kluwer Medknow Publications
2019
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Online Access: | https://doi.org/10.4103/2221-1691.250264 https://doaj.org/article/b81f3929eaf541fdb6883e5303274589 |
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ftdoajarticles:oai:doaj.org/article:b81f3929eaf541fdb6883e5303274589 2023-05-15T15:14:23+02:00 Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) Francisca Sâmara Muniz dos Santos José Weverton Almeida Bezerra Jean Paul Kamdem Aline Augusti Boligon Marli Matiko Anraku Ana Raquel Pereira da Silva Kleber Ribeiro Fidelis Nadghia Figueiredo Leite Antônio Ivanildo Pinho Henrique Douglas Melo Coutinho José Edilson Gonçalves dos Santos 2019-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.250264 https://doaj.org/article/b81f3929eaf541fdb6883e5303274589 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=1;spage=12;epage=17;aulast=dos https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.250264 https://doaj.org/article/b81f3929eaf541fdb6883e5303274589 Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 1, Pp 12-17 (2019) tarenaya spinosa cleome spinose nauphoeta cinerea synergism antibiotic resistance Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.4103/2221-1691.250264 2022-12-31T03:25:37Z Objective: To evaluate the antibacterial activity and neuroprotective capacity of the ethanolic and aqueous extracts of Tarenaya spinosa (T. spinosa) as well as to determine and quantify some of its polyphenols by high performance liquid chromatography with diode-array detection (HPLC-DAD). Methods: The bacterial Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa strains, grown in Heart Agar Infusion, were tested. The drugs gentamicin, norfloxacin and imipenem were used to evaluate the modulating or antagonistic capacity of the T. spinosa extracts. The extract was analysed by HPLC-DAD to determine the main phenolic compounds. For the cell viability tests, individual heads of the Nauphoeta cinerea arthropod model were removed, homogenized in Trifluoromethyl ketone and centrifuged afterwards. Subsequently, 20 μL of NaNO2 were added to the biological material, except in the control group, to evaluate the protection capacity of the extracts. The homogenate of the insect heads was incubated for 2 h in tubes containing tetrazolium bromide. Results: HPLC-DAD demonstrated that the ethanolic extract of T. spinosa presented caffeic acid as the major compound. The ethanolic extract also showed neuroprotective effects at concentrations ≥ 10 μg/mL, while aqueous extract was shown to have a protective effect only at the concentration of 100 μg/mL. The aqueous extract demonstrated a clinically relevant antibacterial activity against the Staphylococcus aureus multidrug resistant strain - MDR, with MIC 512 μg/mL. However, when the extracts were associated with gentamicin and imipenem, a synergism was detected against Staphylococcus aureus and Escherichia coli MDR strains. Conclusions: Although it does not present an antibacterial action, the extracts of T. spinosa can be used in the pharmaceutical industries since its extracts show modulating action of drugs. Besides, these natural products have neuroprotective capacity. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 9 1 12 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
tarenaya spinosa cleome spinose nauphoeta cinerea synergism antibiotic resistance Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
tarenaya spinosa cleome spinose nauphoeta cinerea synergism antibiotic resistance Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Francisca Sâmara Muniz dos Santos José Weverton Almeida Bezerra Jean Paul Kamdem Aline Augusti Boligon Marli Matiko Anraku Ana Raquel Pereira da Silva Kleber Ribeiro Fidelis Nadghia Figueiredo Leite Antônio Ivanildo Pinho Henrique Douglas Melo Coutinho José Edilson Gonçalves dos Santos Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) |
topic_facet |
tarenaya spinosa cleome spinose nauphoeta cinerea synergism antibiotic resistance Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the antibacterial activity and neuroprotective capacity of the ethanolic and aqueous extracts of Tarenaya spinosa (T. spinosa) as well as to determine and quantify some of its polyphenols by high performance liquid chromatography with diode-array detection (HPLC-DAD). Methods: The bacterial Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa strains, grown in Heart Agar Infusion, were tested. The drugs gentamicin, norfloxacin and imipenem were used to evaluate the modulating or antagonistic capacity of the T. spinosa extracts. The extract was analysed by HPLC-DAD to determine the main phenolic compounds. For the cell viability tests, individual heads of the Nauphoeta cinerea arthropod model were removed, homogenized in Trifluoromethyl ketone and centrifuged afterwards. Subsequently, 20 μL of NaNO2 were added to the biological material, except in the control group, to evaluate the protection capacity of the extracts. The homogenate of the insect heads was incubated for 2 h in tubes containing tetrazolium bromide. Results: HPLC-DAD demonstrated that the ethanolic extract of T. spinosa presented caffeic acid as the major compound. The ethanolic extract also showed neuroprotective effects at concentrations ≥ 10 μg/mL, while aqueous extract was shown to have a protective effect only at the concentration of 100 μg/mL. The aqueous extract demonstrated a clinically relevant antibacterial activity against the Staphylococcus aureus multidrug resistant strain - MDR, with MIC 512 μg/mL. However, when the extracts were associated with gentamicin and imipenem, a synergism was detected against Staphylococcus aureus and Escherichia coli MDR strains. Conclusions: Although it does not present an antibacterial action, the extracts of T. spinosa can be used in the pharmaceutical industries since its extracts show modulating action of drugs. Besides, these natural products have neuroprotective capacity. |
format |
Article in Journal/Newspaper |
author |
Francisca Sâmara Muniz dos Santos José Weverton Almeida Bezerra Jean Paul Kamdem Aline Augusti Boligon Marli Matiko Anraku Ana Raquel Pereira da Silva Kleber Ribeiro Fidelis Nadghia Figueiredo Leite Antônio Ivanildo Pinho Henrique Douglas Melo Coutinho José Edilson Gonçalves dos Santos |
author_facet |
Francisca Sâmara Muniz dos Santos José Weverton Almeida Bezerra Jean Paul Kamdem Aline Augusti Boligon Marli Matiko Anraku Ana Raquel Pereira da Silva Kleber Ribeiro Fidelis Nadghia Figueiredo Leite Antônio Ivanildo Pinho Henrique Douglas Melo Coutinho José Edilson Gonçalves dos Santos |
author_sort |
Francisca Sâmara Muniz dos Santos |
title |
Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) |
title_short |
Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) |
title_full |
Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) |
title_fullStr |
Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) |
title_full_unstemmed |
Polyphenolic composition, antibacterial, modulator and neuroprotective activity of Tarenaya spinosa (Jacq.) Raf. (Cleomaceae) |
title_sort |
polyphenolic composition, antibacterial, modulator and neuroprotective activity of tarenaya spinosa (jacq.) raf. (cleomaceae) |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/2221-1691.250264 https://doaj.org/article/b81f3929eaf541fdb6883e5303274589 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 1, Pp 12-17 (2019) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=1;spage=12;epage=17;aulast=dos https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.250264 https://doaj.org/article/b81f3929eaf541fdb6883e5303274589 |
op_doi |
https://doi.org/10.4103/2221-1691.250264 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
9 |
container_issue |
1 |
container_start_page |
12 |
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1766344839931101184 |