New Trypanosoma evansi Type B Isolates from Ethiopian Dromedary Camels.
BACKGROUND:Trypanosoma (T.) evansi is a dyskinetoplastic variant of T. brucei that has gained the ability to be transmitted by all sorts of biting flies. T. evansi can be divided into type A, which is the most abundant and found in Africa, Asia and Latin America and type B, which has so far been iso...
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ftdoajarticles:oai:doaj.org/article:b81f1716b94e49f28be886c19d2b6033 2023-05-15T15:12:38+02:00 New Trypanosoma evansi Type B Isolates from Ethiopian Dromedary Camels. Hadush Birhanu Tadesse Gebrehiwot Bruno Maria Goddeeris Philippe Büscher Nick Van Reet 2016-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004556 https://doaj.org/article/b81f1716b94e49f28be886c19d2b6033 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4818106?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004556 https://doaj.org/article/b81f1716b94e49f28be886c19d2b6033 PLoS Neglected Tropical Diseases, Vol 10, Iss 4, p e0004556 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004556 2022-12-31T12:07:08Z BACKGROUND:Trypanosoma (T.) evansi is a dyskinetoplastic variant of T. brucei that has gained the ability to be transmitted by all sorts of biting flies. T. evansi can be divided into type A, which is the most abundant and found in Africa, Asia and Latin America and type B, which has so far been isolated only from Kenyan dromedary camels. This study aimed at the isolation and the genetic and phenotypic characterisation of type A and B T. evansi stocks from camels in Northern Ethiopia. METHODOLOGY/PRINCIPAL FINDINGS:T. evansi was isolated in mice by inoculation with the cryopreserved buffy coat of parasitologically confirmed animals. Fourteen stocks were thus isolated and subject to genotyping with PCRs targeting type-specific variant surface glycoprotein genes, mitochondrial minicircles and maxicircles, minisatellite markers and the F1-ATP synthase γ subunit gene. Nine stocks corresponded to type A, two stocks were type B and three stocks represented mixed infections between A and B, but not hybrids. One T. evansi type A stock was completely akinetoplastic. Five stocks were adapted to in vitro culture and subjected to a drug sensitivity assay with melarsomine dihydrochloride, diminazene diaceturate, isometamidium chloride and suramin. In vitro adaptation induced some loss of kinetoplasts within 60 days. No correlation between drug sensitivity and absence of the kinetoplast was observed. Sequencing the full coding sequence of the F1-ATP synthase γ subunit revealed new type-specific single nucleotide polymorphisms and deletions. CONCLUSIONS/SIGNIFICANCE:This study addresses some limitations of current molecular markers for T. evansi genotyping. Polymorphism within the F1-ATP synthase γ subunit gene may provide new markers to identify the T. evansi type that do not rely on variant surface glycoprotein genes or kinetoplast DNA. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Dromedary ENVELOPE(163.033,163.033,-78.317,-78.317) PLOS Neglected Tropical Diseases 10 4 e0004556 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Hadush Birhanu Tadesse Gebrehiwot Bruno Maria Goddeeris Philippe Büscher Nick Van Reet New Trypanosoma evansi Type B Isolates from Ethiopian Dromedary Camels. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Trypanosoma (T.) evansi is a dyskinetoplastic variant of T. brucei that has gained the ability to be transmitted by all sorts of biting flies. T. evansi can be divided into type A, which is the most abundant and found in Africa, Asia and Latin America and type B, which has so far been isolated only from Kenyan dromedary camels. This study aimed at the isolation and the genetic and phenotypic characterisation of type A and B T. evansi stocks from camels in Northern Ethiopia. METHODOLOGY/PRINCIPAL FINDINGS:T. evansi was isolated in mice by inoculation with the cryopreserved buffy coat of parasitologically confirmed animals. Fourteen stocks were thus isolated and subject to genotyping with PCRs targeting type-specific variant surface glycoprotein genes, mitochondrial minicircles and maxicircles, minisatellite markers and the F1-ATP synthase γ subunit gene. Nine stocks corresponded to type A, two stocks were type B and three stocks represented mixed infections between A and B, but not hybrids. One T. evansi type A stock was completely akinetoplastic. Five stocks were adapted to in vitro culture and subjected to a drug sensitivity assay with melarsomine dihydrochloride, diminazene diaceturate, isometamidium chloride and suramin. In vitro adaptation induced some loss of kinetoplasts within 60 days. No correlation between drug sensitivity and absence of the kinetoplast was observed. Sequencing the full coding sequence of the F1-ATP synthase γ subunit revealed new type-specific single nucleotide polymorphisms and deletions. CONCLUSIONS/SIGNIFICANCE:This study addresses some limitations of current molecular markers for T. evansi genotyping. Polymorphism within the F1-ATP synthase γ subunit gene may provide new markers to identify the T. evansi type that do not rely on variant surface glycoprotein genes or kinetoplast DNA. |
format |
Article in Journal/Newspaper |
author |
Hadush Birhanu Tadesse Gebrehiwot Bruno Maria Goddeeris Philippe Büscher Nick Van Reet |
author_facet |
Hadush Birhanu Tadesse Gebrehiwot Bruno Maria Goddeeris Philippe Büscher Nick Van Reet |
author_sort |
Hadush Birhanu |
title |
New Trypanosoma evansi Type B Isolates from Ethiopian Dromedary Camels. |
title_short |
New Trypanosoma evansi Type B Isolates from Ethiopian Dromedary Camels. |
title_full |
New Trypanosoma evansi Type B Isolates from Ethiopian Dromedary Camels. |
title_fullStr |
New Trypanosoma evansi Type B Isolates from Ethiopian Dromedary Camels. |
title_full_unstemmed |
New Trypanosoma evansi Type B Isolates from Ethiopian Dromedary Camels. |
title_sort |
new trypanosoma evansi type b isolates from ethiopian dromedary camels. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004556 https://doaj.org/article/b81f1716b94e49f28be886c19d2b6033 |
long_lat |
ENVELOPE(163.033,163.033,-78.317,-78.317) |
geographic |
Arctic Dromedary |
geographic_facet |
Arctic Dromedary |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 4, p e0004556 (2016) |
op_relation |
http://europepmc.org/articles/PMC4818106?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004556 https://doaj.org/article/b81f1716b94e49f28be886c19d2b6033 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004556 |
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PLOS Neglected Tropical Diseases |
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10 |
container_issue |
4 |
container_start_page |
e0004556 |
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1766343292081930240 |