Reference genes for quantitative gene expression studies in multiple avian species.

• Published in: PLoS ONE
• Main Authors: Philipp Olias, Iris Adam, Anne Meyer, Constance Scharff, Achim D Gruber
• Format: Article in Journal/Newspaper
• Language: English
• Published: Public Library of Science (PLoS) 2014
• Subjects: Medicine; R; Science; Q; Finch; Haliaeetus albicilla; White-tailed eagle
• Online Access: https://doi.org/10.1371/journal.pone.0099678; https://doaj.org/article/b806f295b73c4f0e9881fbe42dff4c22

Quantitative real-time PCR (qPCR) rapidly and reliably quantifies gene expression levels across different experimental conditions. Selection of suitable reference genes is essential for meaningful normalization and thus correct interpretation of data. In recent years, an increasing number of avian s...