DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes
Objective: To investigate the phytochemical constituents, antioxidant and anthelmintic activities of the crude methanol extract of Cissus populnea (C. populnea) rhizomes. Methods: Phytochemical screening was performed using standard protocols, and column chromatography of silica gel was used for the...
Published in: | Asian Pacific Journal of Tropical Medicine |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2018
|
Subjects: | |
Online Access: | https://doi.org/10.4103/1995-7645.231468 https://doaj.org/article/b7db1ce7ca11459b9e95b4c756fe6323 |
id |
ftdoajarticles:oai:doaj.org/article:b7db1ce7ca11459b9e95b4c756fe6323 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:b7db1ce7ca11459b9e95b4c756fe6323 2023-05-15T15:11:07+02:00 DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes Jean Noël Nyemb Roland Ndoubalem Emmanuel Talla Alembert Tiabou Tchinda Dieudonné Ndjonka Celine Henoumont Sophie Laurent Joseph Tanyi Mbafor 2018-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.231468 https://doaj.org/article/b7db1ce7ca11459b9e95b4c756fe6323 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=4;spage=280;epage=284;aulast=Nyemb https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.231468 https://doaj.org/article/b7db1ce7ca11459b9e95b4c756fe6323 Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 4, Pp 280-284 (2018) cissus populnea onchocerca ochengi antioxidant phenolic compounds phytochemical constituent bergenin Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.4103/1995-7645.231468 2022-12-31T05:38:54Z Objective: To investigate the phytochemical constituents, antioxidant and anthelmintic activities of the crude methanol extract of Cissus populnea (C. populnea) rhizomes. Methods: Phytochemical screening was performed using standard protocols, and column chromatography of silica gel was used for the compounds isolation. DPPH antiradical scavenging assay was performed in order to evaluate the antioxidant activity. Total phenolic content was evaluated using the Folin–Ciocalteu assay. The anthelmintic activity was screened on the bovine adult male forms of parasitic nematode Onchocerca ochengi, by the in vitro evaluation of the inhibition of adult worm motility and mortality. Worms were incubated in the presence of different concentrations of the plant extract and effects on survival were monitored after 24 and 48 h. Results: The preliminary phytochemical screening revealed the presence of phenolic compounds, saponins, steroids, tannins, and terpenoids. Bergenin and a mixture of phytosterol, β -sitosterol and stigmasterol were isolated from this extract and were identified by nuclear magnetic resonance, mass spectrometry and by comparison with published data. The crude methanol extract of C. populnea rhizomes showed a strong DPPH antiradical activity with a good amount of total phenolic content ((20.69±2.13) g gallic acid equivalent/100 g of extract) and significant anthelmintic activity comparable to the standard drug ivermectin. Bergenin was found to be inactive even after 72 h of incubation. Conclusions: This study constitutes the first report on the anthelmintic activity of this plant and supports the traditional use of C. populnea as a natural antioxidant and anthelmintic. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 11 4 280 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
cissus populnea onchocerca ochengi antioxidant phenolic compounds phytochemical constituent bergenin Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
cissus populnea onchocerca ochengi antioxidant phenolic compounds phytochemical constituent bergenin Arctic medicine. Tropical medicine RC955-962 Jean Noël Nyemb Roland Ndoubalem Emmanuel Talla Alembert Tiabou Tchinda Dieudonné Ndjonka Celine Henoumont Sophie Laurent Joseph Tanyi Mbafor DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes |
topic_facet |
cissus populnea onchocerca ochengi antioxidant phenolic compounds phytochemical constituent bergenin Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To investigate the phytochemical constituents, antioxidant and anthelmintic activities of the crude methanol extract of Cissus populnea (C. populnea) rhizomes. Methods: Phytochemical screening was performed using standard protocols, and column chromatography of silica gel was used for the compounds isolation. DPPH antiradical scavenging assay was performed in order to evaluate the antioxidant activity. Total phenolic content was evaluated using the Folin–Ciocalteu assay. The anthelmintic activity was screened on the bovine adult male forms of parasitic nematode Onchocerca ochengi, by the in vitro evaluation of the inhibition of adult worm motility and mortality. Worms were incubated in the presence of different concentrations of the plant extract and effects on survival were monitored after 24 and 48 h. Results: The preliminary phytochemical screening revealed the presence of phenolic compounds, saponins, steroids, tannins, and terpenoids. Bergenin and a mixture of phytosterol, β -sitosterol and stigmasterol were isolated from this extract and were identified by nuclear magnetic resonance, mass spectrometry and by comparison with published data. The crude methanol extract of C. populnea rhizomes showed a strong DPPH antiradical activity with a good amount of total phenolic content ((20.69±2.13) g gallic acid equivalent/100 g of extract) and significant anthelmintic activity comparable to the standard drug ivermectin. Bergenin was found to be inactive even after 72 h of incubation. Conclusions: This study constitutes the first report on the anthelmintic activity of this plant and supports the traditional use of C. populnea as a natural antioxidant and anthelmintic. |
format |
Article in Journal/Newspaper |
author |
Jean Noël Nyemb Roland Ndoubalem Emmanuel Talla Alembert Tiabou Tchinda Dieudonné Ndjonka Celine Henoumont Sophie Laurent Joseph Tanyi Mbafor |
author_facet |
Jean Noël Nyemb Roland Ndoubalem Emmanuel Talla Alembert Tiabou Tchinda Dieudonné Ndjonka Celine Henoumont Sophie Laurent Joseph Tanyi Mbafor |
author_sort |
Jean Noël Nyemb |
title |
DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes |
title_short |
DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes |
title_full |
DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes |
title_fullStr |
DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes |
title_full_unstemmed |
DPPH antiradical scavenging, anthelmintic and phytochemical studies of Cissus poulnea rhizomes |
title_sort |
dpph antiradical scavenging, anthelmintic and phytochemical studies of cissus poulnea rhizomes |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/1995-7645.231468 https://doaj.org/article/b7db1ce7ca11459b9e95b4c756fe6323 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 4, Pp 280-284 (2018) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=4;spage=280;epage=284;aulast=Nyemb https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.231468 https://doaj.org/article/b7db1ce7ca11459b9e95b4c756fe6323 |
op_doi |
https://doi.org/10.4103/1995-7645.231468 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
11 |
container_issue |
4 |
container_start_page |
280 |
_version_ |
1766342022845693952 |