Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study.
Chronic soil-transmitted helminth (STH) infections are associated with effects on systemic immune responses that could be caused by alterations in immune homeostasis. To investigate this, we measured the impact in children of STH infections on cytokine responses and gene expression in unstimulated b...
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ftdoajarticles:oai:doaj.org/article:b7b2cc848d4346f7aa8a8b861f3d8716 2023-05-15T15:16:05+02:00 Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. Miguel Reina Ortiz Fernanda Schreiber Susana Benitez Nely Broncano Martha E Chico Maritza Vaca Neal Alexander David J Lewis Gordon Dougan Philip J Cooper 2011-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001157 https://doaj.org/article/b7b2cc848d4346f7aa8a8b861f3d8716 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3110165?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001157 https://doaj.org/article/b7b2cc848d4346f7aa8a8b861f3d8716 PLoS Neglected Tropical Diseases, Vol 5, Iss 6, p e1157 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001157 2022-12-31T12:36:22Z Chronic soil-transmitted helminth (STH) infections are associated with effects on systemic immune responses that could be caused by alterations in immune homeostasis. To investigate this, we measured the impact in children of STH infections on cytokine responses and gene expression in unstimulated blood.Sixty children were classified as having chronic, light, or no STH infections. Peripheral blood mononuclear cells were cultured in medium for 5 days to measure cytokine accumulation. RNA was isolated from peripheral blood and gene expression analysed using microarrays. Different infection groups were compared for the purpose of analysis: STH infection (combined chronic and light vs. uninfected groups) and chronic STH infection (chronic vs. combined light and uninfected groups). The chronic STH infection effect was associated with elevated production of GM-CSF (P=0.007), IL-2 (P=0.03), IL-5 (P=0.01), and IL-10 (P=0.01). Data reduction suggested that chronic infections were primarily associated with an immune phenotype characterized by elevated IL-5 and IL-10, typical of a modified Th2-like response. Chronic STH infections were associated with the up-regulation of genes associated with immune homeostasis (IDO, P=0.03; CCL23, P=0.008, HRK, P=0.005), down-regulation of microRNA hsa-let-7d (P=0.01) and differential regulation of several genes associated with granulocyte-mediated inflammation (IL-8, down-regulated, P=0.0002; RNASE2, up-regulated, P=0.009; RNASE3, up-regulated, p=0.03).Chronic STH infections were associated with a cytokine response indicative of a modified Th2 response. There was evidence that STH infections were associated with a pattern of gene expression suggestive of the induction of homeostatic mechanisms, the differential expression of several inflammatory genes and the down-regulation of microRNA has-let-7d. Effects on immune homeostasis and the development of a modified Th2 immune response during chronic STH infections could explain the systemic immunologic effects that have been associated with ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 6 e1157 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Miguel Reina Ortiz Fernanda Schreiber Susana Benitez Nely Broncano Martha E Chico Maritza Vaca Neal Alexander David J Lewis Gordon Dougan Philip J Cooper Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Chronic soil-transmitted helminth (STH) infections are associated with effects on systemic immune responses that could be caused by alterations in immune homeostasis. To investigate this, we measured the impact in children of STH infections on cytokine responses and gene expression in unstimulated blood.Sixty children were classified as having chronic, light, or no STH infections. Peripheral blood mononuclear cells were cultured in medium for 5 days to measure cytokine accumulation. RNA was isolated from peripheral blood and gene expression analysed using microarrays. Different infection groups were compared for the purpose of analysis: STH infection (combined chronic and light vs. uninfected groups) and chronic STH infection (chronic vs. combined light and uninfected groups). The chronic STH infection effect was associated with elevated production of GM-CSF (P=0.007), IL-2 (P=0.03), IL-5 (P=0.01), and IL-10 (P=0.01). Data reduction suggested that chronic infections were primarily associated with an immune phenotype characterized by elevated IL-5 and IL-10, typical of a modified Th2-like response. Chronic STH infections were associated with the up-regulation of genes associated with immune homeostasis (IDO, P=0.03; CCL23, P=0.008, HRK, P=0.005), down-regulation of microRNA hsa-let-7d (P=0.01) and differential regulation of several genes associated with granulocyte-mediated inflammation (IL-8, down-regulated, P=0.0002; RNASE2, up-regulated, P=0.009; RNASE3, up-regulated, p=0.03).Chronic STH infections were associated with a cytokine response indicative of a modified Th2 response. There was evidence that STH infections were associated with a pattern of gene expression suggestive of the induction of homeostatic mechanisms, the differential expression of several inflammatory genes and the down-regulation of microRNA has-let-7d. Effects on immune homeostasis and the development of a modified Th2 immune response during chronic STH infections could explain the systemic immunologic effects that have been associated with ... |
format |
Article in Journal/Newspaper |
author |
Miguel Reina Ortiz Fernanda Schreiber Susana Benitez Nely Broncano Martha E Chico Maritza Vaca Neal Alexander David J Lewis Gordon Dougan Philip J Cooper |
author_facet |
Miguel Reina Ortiz Fernanda Schreiber Susana Benitez Nely Broncano Martha E Chico Maritza Vaca Neal Alexander David J Lewis Gordon Dougan Philip J Cooper |
author_sort |
Miguel Reina Ortiz |
title |
Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. |
title_short |
Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. |
title_full |
Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. |
title_fullStr |
Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. |
title_full_unstemmed |
Effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. |
title_sort |
effects of chronic ascariasis and trichuriasis on cytokine production and gene expression in human blood: a cross-sectional study. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001157 https://doaj.org/article/b7b2cc848d4346f7aa8a8b861f3d8716 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 6, p e1157 (2011) |
op_relation |
http://europepmc.org/articles/PMC3110165?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001157 https://doaj.org/article/b7b2cc848d4346f7aa8a8b861f3d8716 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001157 |
container_title |
PLoS Neglected Tropical Diseases |
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5 |
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6 |
container_start_page |
e1157 |
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1766346390395420672 |