Novel, meso-substituted cationic porphyrin molecule for photo-mediated larval control of the dengue vector Aedes aegypti.
BACKGROUND: Control of the mosquito vector population is the most effective strategy currently available for the prevention of dengue fever and the containment of outbreaks. Photo-activated oxidants may represent promising tools for developing effective, safe and ecofriendly novel larvicides. The pu...
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ftdoajarticles:oai:doaj.org/article:b78d7c20bce846d5ac0eb9434397626f 2023-05-15T15:14:16+02:00 Novel, meso-substituted cationic porphyrin molecule for photo-mediated larval control of the dengue vector Aedes aegypti. Leonardo Lucantoni Michela Magaraggia Giulio Lupidi Robert Kossivi Ouedraogo Olimpia Coppellotti Fulvio Esposito Clara Fabris Giulio Jori Annette Habluetzel 2011-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001434 https://doaj.org/article/b78d7c20bce846d5ac0eb9434397626f EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3243718?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001434 https://doaj.org/article/b78d7c20bce846d5ac0eb9434397626f PLoS Neglected Tropical Diseases, Vol 5, Iss 12, p e1434 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001434 2022-12-31T01:31:46Z BACKGROUND: Control of the mosquito vector population is the most effective strategy currently available for the prevention of dengue fever and the containment of outbreaks. Photo-activated oxidants may represent promising tools for developing effective, safe and ecofriendly novel larvicides. The purpose of this study was to evaluate the potential of the synthetic meso-substituted porphyrin meso-tri(N-methylpyridyl), meso-mono(N-tetradecylpyridyl)porphine (C14) as a photoactivatable larvicide against the dengue vector Aedes (Stegomyia) aegypti. METHODOLOGY: The photophysical and photochemical properties of the C14 molecule were assessed spectrophotometrically. Photomediated larvicidal efficacy, route of intake and site of action were determined on Ae. aegypti larvae by laboratory bioassays and fluorescence microscopy. Using powdered food pellet for laboratory rodents (a common larval food used in the laboratory) as a carrier for C14, loading-release dynamics, larvicidal efficacy and residual activity of the C14-carrier complex were investigated. MAIN FINDINGS: The C14 molecule was found to exert a potent photosensitizing activity on Ae. aegypti larvae. At irradiation intervals of 12 h and 1 h, at a light intensity of 4.0 mW/cm(2), which is 50-100 times lower than that of natural sunlight, LC(50) values of 0.1 µM (0.15 mg/l) and 0.5 µM (0.77 mg/l) were obtained, respectively. The molecule was active after ingestion by the larvae and caused irreversible, lethal damage to the midgut and caecal epithelia. The amphiphilic nature of C14 allowed a formulate to be produced that not only was as active against the larvae as C14 in solution, but also possessed a residual activity of at least two weeks, in laboratory conditions. CONCLUSIONS: The meso-substituted synthetic porphyrin C14, thanks to its photo-sensitizing properties represents an attractive candidate for the development of novel photolarvicides for dengue vector control. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 12 e1434 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Leonardo Lucantoni Michela Magaraggia Giulio Lupidi Robert Kossivi Ouedraogo Olimpia Coppellotti Fulvio Esposito Clara Fabris Giulio Jori Annette Habluetzel Novel, meso-substituted cationic porphyrin molecule for photo-mediated larval control of the dengue vector Aedes aegypti. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Control of the mosquito vector population is the most effective strategy currently available for the prevention of dengue fever and the containment of outbreaks. Photo-activated oxidants may represent promising tools for developing effective, safe and ecofriendly novel larvicides. The purpose of this study was to evaluate the potential of the synthetic meso-substituted porphyrin meso-tri(N-methylpyridyl), meso-mono(N-tetradecylpyridyl)porphine (C14) as a photoactivatable larvicide against the dengue vector Aedes (Stegomyia) aegypti. METHODOLOGY: The photophysical and photochemical properties of the C14 molecule were assessed spectrophotometrically. Photomediated larvicidal efficacy, route of intake and site of action were determined on Ae. aegypti larvae by laboratory bioassays and fluorescence microscopy. Using powdered food pellet for laboratory rodents (a common larval food used in the laboratory) as a carrier for C14, loading-release dynamics, larvicidal efficacy and residual activity of the C14-carrier complex were investigated. MAIN FINDINGS: The C14 molecule was found to exert a potent photosensitizing activity on Ae. aegypti larvae. At irradiation intervals of 12 h and 1 h, at a light intensity of 4.0 mW/cm(2), which is 50-100 times lower than that of natural sunlight, LC(50) values of 0.1 µM (0.15 mg/l) and 0.5 µM (0.77 mg/l) were obtained, respectively. The molecule was active after ingestion by the larvae and caused irreversible, lethal damage to the midgut and caecal epithelia. The amphiphilic nature of C14 allowed a formulate to be produced that not only was as active against the larvae as C14 in solution, but also possessed a residual activity of at least two weeks, in laboratory conditions. CONCLUSIONS: The meso-substituted synthetic porphyrin C14, thanks to its photo-sensitizing properties represents an attractive candidate for the development of novel photolarvicides for dengue vector control. |
format |
Article in Journal/Newspaper |
author |
Leonardo Lucantoni Michela Magaraggia Giulio Lupidi Robert Kossivi Ouedraogo Olimpia Coppellotti Fulvio Esposito Clara Fabris Giulio Jori Annette Habluetzel |
author_facet |
Leonardo Lucantoni Michela Magaraggia Giulio Lupidi Robert Kossivi Ouedraogo Olimpia Coppellotti Fulvio Esposito Clara Fabris Giulio Jori Annette Habluetzel |
author_sort |
Leonardo Lucantoni |
title |
Novel, meso-substituted cationic porphyrin molecule for photo-mediated larval control of the dengue vector Aedes aegypti. |
title_short |
Novel, meso-substituted cationic porphyrin molecule for photo-mediated larval control of the dengue vector Aedes aegypti. |
title_full |
Novel, meso-substituted cationic porphyrin molecule for photo-mediated larval control of the dengue vector Aedes aegypti. |
title_fullStr |
Novel, meso-substituted cationic porphyrin molecule for photo-mediated larval control of the dengue vector Aedes aegypti. |
title_full_unstemmed |
Novel, meso-substituted cationic porphyrin molecule for photo-mediated larval control of the dengue vector Aedes aegypti. |
title_sort |
novel, meso-substituted cationic porphyrin molecule for photo-mediated larval control of the dengue vector aedes aegypti. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001434 https://doaj.org/article/b78d7c20bce846d5ac0eb9434397626f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 12, p e1434 (2011) |
op_relation |
http://europepmc.org/articles/PMC3243718?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001434 https://doaj.org/article/b78d7c20bce846d5ac0eb9434397626f |
op_doi |
https://doi.org/10.1371/journal.pntd.0001434 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
12 |
container_start_page |
e1434 |
_version_ |
1766344732760342528 |