Persistence of E. coli O157:H7 in Frozen Soils: Role of Freezing Temperature
Soilborne pathogen infections are increasingly reported globally in recent years. Infectious agents have contaminated most of seasonal frozen zone and have been found in permafrost due to the effects of intensified human activities on global warming. Therefore, in regard to sustainable agriculture,...
Published in: | Sustainability |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2023
|
Subjects: | |
Online Access: | https://doi.org/10.3390/su151713249 https://doaj.org/article/b779a5faa2214a7da71ca0d65f99494d |
id |
ftdoajarticles:oai:doaj.org/article:b779a5faa2214a7da71ca0d65f99494d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:b779a5faa2214a7da71ca0d65f99494d 2023-10-09T21:55:14+02:00 Persistence of E. coli O157:H7 in Frozen Soils: Role of Freezing Temperature Jiawei Wang Jiafen Liao Jinhua Ma Guangze Lyu Xiaoyin Yang Abasiofiok M. Ibekwe Jincai Ma 2023-09-01T00:00:00Z https://doi.org/10.3390/su151713249 https://doaj.org/article/b779a5faa2214a7da71ca0d65f99494d EN eng MDPI AG https://www.mdpi.com/2071-1050/15/17/13249 https://doaj.org/toc/2071-1050 doi:10.3390/su151713249 2071-1050 https://doaj.org/article/b779a5faa2214a7da71ca0d65f99494d Sustainability, Vol 15, Iss 13249, p 13249 (2023) soil freezing temperature moisture E. coli O157:H7 persistence Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 article 2023 ftdoajarticles https://doi.org/10.3390/su151713249 2023-09-10T00:34:34Z Soilborne pathogen infections are increasingly reported globally in recent years. Infectious agents have contaminated most of seasonal frozen zone and have been found in permafrost due to the effects of intensified human activities on global warming. Therefore, in regard to sustainable agriculture, it is particularly important to assess the environmental behavior of those pathogens in frozen soils. Due to high pathogenicity and low infection threshold, E. coli O157:H7 (EcO157) is a worldwide public health concern, and recent studies have focused more on its fate in the environment. The survival of this serotype in a large variety of environmental media under temperature above 0 °C has been investigated, while its persistence in frozen soils has received less attention. In this study, we collected soils with different textures from a seasonally frozen zone in northeast China and investigated the persistence of EcO157 in soils at freezing temperatures (−5 °C and −15 °C) and moisture content (30% and 60% water holding capacity (WHC)) of the soils. By fitting the survival data with a Weibull model, we obtained three parameters: first log reduction time ( δ in days), survival curve shape parameters ( p ), and monthly average reduction in EcO157 ( MAR , log·gdw −1 ·mon −1 ). The results showed that temperature has a major impact on persistence, while moisture content has less effect on the survival of EcO157. Further multi-variable analysis revealed that the physical and chemical properties (e.g., sand fraction) of soil play an important role in survival. Certain bacterial groups are significantly correlated with the survival of EcO157 in frozen soils at −5 °C but not for the ones incubated at −15 °C. Our data could provide background data to evaluate the health risk associated with EcO157. The results could be helpful to improve sustainable soil practices and to develop regulations and policies aiming to achieve sustainable agriculture. Article in Journal/Newspaper permafrost Directory of Open Access Journals: DOAJ Articles Sustainability 15 17 13249 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
soil freezing temperature moisture E. coli O157:H7 persistence Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 |
spellingShingle |
soil freezing temperature moisture E. coli O157:H7 persistence Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 Jiawei Wang Jiafen Liao Jinhua Ma Guangze Lyu Xiaoyin Yang Abasiofiok M. Ibekwe Jincai Ma Persistence of E. coli O157:H7 in Frozen Soils: Role of Freezing Temperature |
topic_facet |
soil freezing temperature moisture E. coli O157:H7 persistence Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 |
description |
Soilborne pathogen infections are increasingly reported globally in recent years. Infectious agents have contaminated most of seasonal frozen zone and have been found in permafrost due to the effects of intensified human activities on global warming. Therefore, in regard to sustainable agriculture, it is particularly important to assess the environmental behavior of those pathogens in frozen soils. Due to high pathogenicity and low infection threshold, E. coli O157:H7 (EcO157) is a worldwide public health concern, and recent studies have focused more on its fate in the environment. The survival of this serotype in a large variety of environmental media under temperature above 0 °C has been investigated, while its persistence in frozen soils has received less attention. In this study, we collected soils with different textures from a seasonally frozen zone in northeast China and investigated the persistence of EcO157 in soils at freezing temperatures (−5 °C and −15 °C) and moisture content (30% and 60% water holding capacity (WHC)) of the soils. By fitting the survival data with a Weibull model, we obtained three parameters: first log reduction time ( δ in days), survival curve shape parameters ( p ), and monthly average reduction in EcO157 ( MAR , log·gdw −1 ·mon −1 ). The results showed that temperature has a major impact on persistence, while moisture content has less effect on the survival of EcO157. Further multi-variable analysis revealed that the physical and chemical properties (e.g., sand fraction) of soil play an important role in survival. Certain bacterial groups are significantly correlated with the survival of EcO157 in frozen soils at −5 °C but not for the ones incubated at −15 °C. Our data could provide background data to evaluate the health risk associated with EcO157. The results could be helpful to improve sustainable soil practices and to develop regulations and policies aiming to achieve sustainable agriculture. |
format |
Article in Journal/Newspaper |
author |
Jiawei Wang Jiafen Liao Jinhua Ma Guangze Lyu Xiaoyin Yang Abasiofiok M. Ibekwe Jincai Ma |
author_facet |
Jiawei Wang Jiafen Liao Jinhua Ma Guangze Lyu Xiaoyin Yang Abasiofiok M. Ibekwe Jincai Ma |
author_sort |
Jiawei Wang |
title |
Persistence of E. coli O157:H7 in Frozen Soils: Role of Freezing Temperature |
title_short |
Persistence of E. coli O157:H7 in Frozen Soils: Role of Freezing Temperature |
title_full |
Persistence of E. coli O157:H7 in Frozen Soils: Role of Freezing Temperature |
title_fullStr |
Persistence of E. coli O157:H7 in Frozen Soils: Role of Freezing Temperature |
title_full_unstemmed |
Persistence of E. coli O157:H7 in Frozen Soils: Role of Freezing Temperature |
title_sort |
persistence of e. coli o157:h7 in frozen soils: role of freezing temperature |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/su151713249 https://doaj.org/article/b779a5faa2214a7da71ca0d65f99494d |
genre |
permafrost |
genre_facet |
permafrost |
op_source |
Sustainability, Vol 15, Iss 13249, p 13249 (2023) |
op_relation |
https://www.mdpi.com/2071-1050/15/17/13249 https://doaj.org/toc/2071-1050 doi:10.3390/su151713249 2071-1050 https://doaj.org/article/b779a5faa2214a7da71ca0d65f99494d |
op_doi |
https://doi.org/10.3390/su151713249 |
container_title |
Sustainability |
container_volume |
15 |
container_issue |
17 |
container_start_page |
13249 |
_version_ |
1779319090285903872 |