Mantle transcriptome sequencing of Mytilus spp. and identification of putative biomineralization genes
In molluscs, the shell secreted by mantle tissue during the biomineralization process is the first barrier against predators and mechanical damage. Changing environmental conditions, such as ocean acidification, influence shell strength and thus protection of the soft body within. Mussels are marine...
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ftdoajarticles:oai:doaj.org/article:b764aad2c7664a46885efa9c58120d7f 2024-01-07T09:45:46+01:00 Mantle transcriptome sequencing of Mytilus spp. and identification of putative biomineralization genes Magdalena Malachowicz Roman Wenne 2019-01-01T00:00:00Z https://doi.org/10.7717/peerj.6245 https://doaj.org/article/b764aad2c7664a46885efa9c58120d7f EN eng PeerJ Inc. https://peerj.com/articles/6245.pdf https://peerj.com/articles/6245/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.6245 2167-8359 https://doaj.org/article/b764aad2c7664a46885efa9c58120d7f PeerJ, Vol 6, p e6245 (2019) Transcriptome Mytilus Mantle Biomineralization Molecular markers Chitinase Medicine R Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.7717/peerj.6245 2023-12-10T01:50:46Z In molluscs, the shell secreted by mantle tissue during the biomineralization process is the first barrier against predators and mechanical damage. Changing environmental conditions, such as ocean acidification, influence shell strength and thus protection of the soft body within. Mussels are marine bivalves with important commercial and ecological value worldwide. Despite this importance, the proteins involved in the biomineralization and pigmentation processes in Mytilus spp. remain unclear, as does taxonomy of Mytilus taxa, though there have been many molecular studies. To further understanding in these areas, this study aimed to characterize and compare mantle transcriptomes of four mussel taxa using next generation sequencing. Mussels representing four taxa, were collected from several localities and RNA from mantle tissue was extracted. RNA sequences obtained were assembled, annotated and potential molecular markers, including simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) were identified. Candidate contigs putatively related to biomineralization and pigmentation processes were then selected and several transcripts were chosen for phylogenetic analyses from the Bivalvia class. Transcriptome comparisons between Mytilus taxa, including gene ontology (GO) enrichment analysis and orthologues identification were performed. Of assembled contigs, 46.57%, 37.28% and 17.53% were annotated using NCBI NR, GO and Kyoto Encyclopedia of Genes and Genomes databases, respectively. Potential SSRs (483) and SNPs (1,497) were identified. Results presented a total of 1,292 contigs putatively involved in biomineralization and melanogenesis. Phylogenetic analyses of α-carbonic anhydrase, chitinase and tyrosinase revealed complex evolutionary history and diversity of these genes, which may be a result of duplication events or adaptation to different environments in mussels and other bivalves. Enrichment analyses revealed GO terms associated with pH and thermal response in Mytilus edulis from the North ... Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles PeerJ 6 e6245 |
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Open Polar |
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language |
English |
topic |
Transcriptome Mytilus Mantle Biomineralization Molecular markers Chitinase Medicine R Biology (General) QH301-705.5 |
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Transcriptome Mytilus Mantle Biomineralization Molecular markers Chitinase Medicine R Biology (General) QH301-705.5 Magdalena Malachowicz Roman Wenne Mantle transcriptome sequencing of Mytilus spp. and identification of putative biomineralization genes |
topic_facet |
Transcriptome Mytilus Mantle Biomineralization Molecular markers Chitinase Medicine R Biology (General) QH301-705.5 |
description |
In molluscs, the shell secreted by mantle tissue during the biomineralization process is the first barrier against predators and mechanical damage. Changing environmental conditions, such as ocean acidification, influence shell strength and thus protection of the soft body within. Mussels are marine bivalves with important commercial and ecological value worldwide. Despite this importance, the proteins involved in the biomineralization and pigmentation processes in Mytilus spp. remain unclear, as does taxonomy of Mytilus taxa, though there have been many molecular studies. To further understanding in these areas, this study aimed to characterize and compare mantle transcriptomes of four mussel taxa using next generation sequencing. Mussels representing four taxa, were collected from several localities and RNA from mantle tissue was extracted. RNA sequences obtained were assembled, annotated and potential molecular markers, including simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) were identified. Candidate contigs putatively related to biomineralization and pigmentation processes were then selected and several transcripts were chosen for phylogenetic analyses from the Bivalvia class. Transcriptome comparisons between Mytilus taxa, including gene ontology (GO) enrichment analysis and orthologues identification were performed. Of assembled contigs, 46.57%, 37.28% and 17.53% were annotated using NCBI NR, GO and Kyoto Encyclopedia of Genes and Genomes databases, respectively. Potential SSRs (483) and SNPs (1,497) were identified. Results presented a total of 1,292 contigs putatively involved in biomineralization and melanogenesis. Phylogenetic analyses of α-carbonic anhydrase, chitinase and tyrosinase revealed complex evolutionary history and diversity of these genes, which may be a result of duplication events or adaptation to different environments in mussels and other bivalves. Enrichment analyses revealed GO terms associated with pH and thermal response in Mytilus edulis from the North ... |
format |
Article in Journal/Newspaper |
author |
Magdalena Malachowicz Roman Wenne |
author_facet |
Magdalena Malachowicz Roman Wenne |
author_sort |
Magdalena Malachowicz |
title |
Mantle transcriptome sequencing of Mytilus spp. and identification of putative biomineralization genes |
title_short |
Mantle transcriptome sequencing of Mytilus spp. and identification of putative biomineralization genes |
title_full |
Mantle transcriptome sequencing of Mytilus spp. and identification of putative biomineralization genes |
title_fullStr |
Mantle transcriptome sequencing of Mytilus spp. and identification of putative biomineralization genes |
title_full_unstemmed |
Mantle transcriptome sequencing of Mytilus spp. and identification of putative biomineralization genes |
title_sort |
mantle transcriptome sequencing of mytilus spp. and identification of putative biomineralization genes |
publisher |
PeerJ Inc. |
publishDate |
2019 |
url |
https://doi.org/10.7717/peerj.6245 https://doaj.org/article/b764aad2c7664a46885efa9c58120d7f |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
PeerJ, Vol 6, p e6245 (2019) |
op_relation |
https://peerj.com/articles/6245.pdf https://peerj.com/articles/6245/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.6245 2167-8359 https://doaj.org/article/b764aad2c7664a46885efa9c58120d7f |
op_doi |
https://doi.org/10.7717/peerj.6245 |
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PeerJ |
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6 |
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e6245 |
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