Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic)
We explored the patterns of total and active bacterial community structure in a gradient covering surface waters from the Mackenzie River to the coastal Beaufort Sea in the Canadian Arctic Ocean, with a particular focus on free-living (FL) vs. particle-attached (PA) communities. Capillary electropho...
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Online Access: | https://doi.org/10.5194/bg-10-2747-2013 https://doaj.org/article/b7277752ec6d4cc8abae735b4a575485 |
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ftdoajarticles:oai:doaj.org/article:b7277752ec6d4cc8abae735b4a575485 2023-05-15T15:02:20+02:00 Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) E. Ortega-Retuerta F. Joux W. H. Jeffrey J. F. Ghiglione 2013-04-01T00:00:00Z https://doi.org/10.5194/bg-10-2747-2013 https://doaj.org/article/b7277752ec6d4cc8abae735b4a575485 EN eng Copernicus Publications http://www.biogeosciences.net/10/2747/2013/bg-10-2747-2013.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-10-2747-2013 1726-4170 1726-4189 https://doaj.org/article/b7277752ec6d4cc8abae735b4a575485 Biogeosciences, Vol 10, Iss 4, Pp 2747-2759 (2013) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2013 ftdoajarticles https://doi.org/10.5194/bg-10-2747-2013 2022-12-31T13:34:01Z We explored the patterns of total and active bacterial community structure in a gradient covering surface waters from the Mackenzie River to the coastal Beaufort Sea in the Canadian Arctic Ocean, with a particular focus on free-living (FL) vs. particle-attached (PA) communities. Capillary electrophoresis–single-strand conformation polymorphism (CE-SSCP) showed significant differences when comparing river, coast and open sea bacterial community structures. In contrast to the river and coastal waters, total (16S rDNA-based) and active (16S rRNA-based) communities in the open sea samples were not significantly different, suggesting that most present bacterial groups were equally active in this area. Additionally, we observed significant differences between PA and FL bacterial community structure in the open sea, but similar structure in the two fractions for coastal and river samples. Direct multivariate statistical analyses showed that total community structure was mainly driven by salinity (a proxy of dissolved organic carbon and chromophoric dissolved organic matter), suspended particles, amino acids and chlorophyll a . Pyrosequencing of 16S rRNA genes from selected samples confirmed significant differences between river, coastal and sea samples. The PA fraction was only different (15.7% similarity) from the FL one in the open sea sample. Furthermore, PA samples generally showed higher diversity (Shannon, Simpson and Chao indices) than FL samples. At the class level, Opitutae was most abundant in the PA fraction of the sea sample, followed by Flavobacteria and Gammaproteobacteria, while the FL sea sample was dominated by Alphaproteobacteria. Finally, for the coast and river samples and both PA and FL fractions, Betaproteobacteria, Alphaproteobacteria and Actinobacteria were dominant. These results highlight the coexistence of particle specialists and generalists and the role of particle quality in structuring bacterial communities in the area. These results may also serve as a basis to predict further changes in ... Article in Journal/Newspaper Arctic Arctic Ocean Beaufort Sea Mackenzie river Directory of Open Access Journals: DOAJ Articles Arctic Arctic Ocean Mackenzie River Biogeosciences 10 4 2747 2759 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
spellingShingle |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 E. Ortega-Retuerta F. Joux W. H. Jeffrey J. F. Ghiglione Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
We explored the patterns of total and active bacterial community structure in a gradient covering surface waters from the Mackenzie River to the coastal Beaufort Sea in the Canadian Arctic Ocean, with a particular focus on free-living (FL) vs. particle-attached (PA) communities. Capillary electrophoresis–single-strand conformation polymorphism (CE-SSCP) showed significant differences when comparing river, coast and open sea bacterial community structures. In contrast to the river and coastal waters, total (16S rDNA-based) and active (16S rRNA-based) communities in the open sea samples were not significantly different, suggesting that most present bacterial groups were equally active in this area. Additionally, we observed significant differences between PA and FL bacterial community structure in the open sea, but similar structure in the two fractions for coastal and river samples. Direct multivariate statistical analyses showed that total community structure was mainly driven by salinity (a proxy of dissolved organic carbon and chromophoric dissolved organic matter), suspended particles, amino acids and chlorophyll a . Pyrosequencing of 16S rRNA genes from selected samples confirmed significant differences between river, coastal and sea samples. The PA fraction was only different (15.7% similarity) from the FL one in the open sea sample. Furthermore, PA samples generally showed higher diversity (Shannon, Simpson and Chao indices) than FL samples. At the class level, Opitutae was most abundant in the PA fraction of the sea sample, followed by Flavobacteria and Gammaproteobacteria, while the FL sea sample was dominated by Alphaproteobacteria. Finally, for the coast and river samples and both PA and FL fractions, Betaproteobacteria, Alphaproteobacteria and Actinobacteria were dominant. These results highlight the coexistence of particle specialists and generalists and the role of particle quality in structuring bacterial communities in the area. These results may also serve as a basis to predict further changes in ... |
format |
Article in Journal/Newspaper |
author |
E. Ortega-Retuerta F. Joux W. H. Jeffrey J. F. Ghiglione |
author_facet |
E. Ortega-Retuerta F. Joux W. H. Jeffrey J. F. Ghiglione |
author_sort |
E. Ortega-Retuerta |
title |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_short |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_full |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_fullStr |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_full_unstemmed |
Spatial variability of particle-attached and free-living bacterial diversity in surface waters from the Mackenzie River to the Beaufort Sea (Canadian Arctic) |
title_sort |
spatial variability of particle-attached and free-living bacterial diversity in surface waters from the mackenzie river to the beaufort sea (canadian arctic) |
publisher |
Copernicus Publications |
publishDate |
2013 |
url |
https://doi.org/10.5194/bg-10-2747-2013 https://doaj.org/article/b7277752ec6d4cc8abae735b4a575485 |
geographic |
Arctic Arctic Ocean Mackenzie River |
geographic_facet |
Arctic Arctic Ocean Mackenzie River |
genre |
Arctic Arctic Ocean Beaufort Sea Mackenzie river |
genre_facet |
Arctic Arctic Ocean Beaufort Sea Mackenzie river |
op_source |
Biogeosciences, Vol 10, Iss 4, Pp 2747-2759 (2013) |
op_relation |
http://www.biogeosciences.net/10/2747/2013/bg-10-2747-2013.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-10-2747-2013 1726-4170 1726-4189 https://doaj.org/article/b7277752ec6d4cc8abae735b4a575485 |
op_doi |
https://doi.org/10.5194/bg-10-2747-2013 |
container_title |
Biogeosciences |
container_volume |
10 |
container_issue |
4 |
container_start_page |
2747 |
op_container_end_page |
2759 |
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1766334308858986496 |