Temperature differently affected methanogenic pathways and microbial communities in sub-Antarctic freshwater ecosystems
Freshwater ecosystems are responsible for an important part of the methane (CH4) emissions which are likely to change with global warming. This study aims to evaluate temperature-induced (from 5 to 20 °C) changes on microbial community structure and methanogenic pathways in five sub-Antarctic lake s...
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ftdoajarticles:oai:doaj.org/article:b71a71c57f45468dae689ed88e5165d1 2023-05-15T13:46:16+02:00 Temperature differently affected methanogenic pathways and microbial communities in sub-Antarctic freshwater ecosystems Céline Lavergne Polette Aguilar-Muñoz Natalia Calle Frédéric Thalasso Maria Soledad Astorga-España Armando Sepulveda-Jauregui Karla Martinez-Cruz Laure Gandois Andrés Mansilla Rolando Chamy Maialen Barret Léa Cabrol 2021-09-01T00:00:00Z https://doi.org/10.1016/j.envint.2021.106575 https://doaj.org/article/b71a71c57f45468dae689ed88e5165d1 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S0160412021002002 https://doaj.org/toc/0160-4120 0160-4120 doi:10.1016/j.envint.2021.106575 https://doaj.org/article/b71a71c57f45468dae689ed88e5165d1 Environment International, Vol 154, Iss , Pp 106575- (2021) 16S rRNA amplicons Archaea Bacteria Limnology Methane Global warming Environmental sciences GE1-350 article 2021 ftdoajarticles https://doi.org/10.1016/j.envint.2021.106575 2022-12-31T12:16:11Z Freshwater ecosystems are responsible for an important part of the methane (CH4) emissions which are likely to change with global warming. This study aims to evaluate temperature-induced (from 5 to 20 °C) changes on microbial community structure and methanogenic pathways in five sub-Antarctic lake sediments from Magallanes strait to Cape Horn, Chile. We combined in situ CH4 flux measurements, CH4 production rates (MPRs), gene abundance quantification and microbial community structure analysis (metabarcoding of the 16S rRNA gene). Under unamended conditions, a temperature increase of 5 °C doubled MPR while microbial community structure was not affected. Stimulation of methanogenesis by methanogenic precursors as acetate and H2/CO2, resulted in an increase of MPRs up to 127-fold and 19-fold, respectively, as well as an enrichment of mcrA-carriers strikingly stronger under acetate amendment. At low temperatures, H2/CO2-derived MPRs were considerably lower (down to 160-fold lower) than the acetate-derived MPRs, but the contribution of hydrogenotrophic methanogenesis increased with temperature. Temperature dependence of MPRs was significantly higher in incubations spiked with H2/CO2 (c. 1.9 eV) compared to incubations spiked with acetate or unamended (c. 0.8 eV). Temperature was not found to shape the total microbial community structure, that rather exhibited a site-specific variability among the studied lakes. However, the methanogenic archaeal community structure was driven by amended methanogenic precursors with a dominance of Methanobacterium in H2/CO2-based incubations and Methanosarcina in acetate-based incubations. We also suggested the importance of acetogenic H2-production outcompeting hydrogenotrohic methanogenesis especially at low temperatures, further supported by homoacetogen proportion in the microcosm communities. The combination of in situ-, and laboratory-based measurements and molecular approaches indicates that the hydrogenotrophic pathway may become more important with increasing temperatures ... Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic Cape Horn ENVELOPE(-135.021,-135.021,61.583,61.583) Magallanes ENVELOPE(-62.933,-62.933,-64.883,-64.883) Environment International 154 106575 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
16S rRNA amplicons Archaea Bacteria Limnology Methane Global warming Environmental sciences GE1-350 |
spellingShingle |
16S rRNA amplicons Archaea Bacteria Limnology Methane Global warming Environmental sciences GE1-350 Céline Lavergne Polette Aguilar-Muñoz Natalia Calle Frédéric Thalasso Maria Soledad Astorga-España Armando Sepulveda-Jauregui Karla Martinez-Cruz Laure Gandois Andrés Mansilla Rolando Chamy Maialen Barret Léa Cabrol Temperature differently affected methanogenic pathways and microbial communities in sub-Antarctic freshwater ecosystems |
topic_facet |
16S rRNA amplicons Archaea Bacteria Limnology Methane Global warming Environmental sciences GE1-350 |
description |
Freshwater ecosystems are responsible for an important part of the methane (CH4) emissions which are likely to change with global warming. This study aims to evaluate temperature-induced (from 5 to 20 °C) changes on microbial community structure and methanogenic pathways in five sub-Antarctic lake sediments from Magallanes strait to Cape Horn, Chile. We combined in situ CH4 flux measurements, CH4 production rates (MPRs), gene abundance quantification and microbial community structure analysis (metabarcoding of the 16S rRNA gene). Under unamended conditions, a temperature increase of 5 °C doubled MPR while microbial community structure was not affected. Stimulation of methanogenesis by methanogenic precursors as acetate and H2/CO2, resulted in an increase of MPRs up to 127-fold and 19-fold, respectively, as well as an enrichment of mcrA-carriers strikingly stronger under acetate amendment. At low temperatures, H2/CO2-derived MPRs were considerably lower (down to 160-fold lower) than the acetate-derived MPRs, but the contribution of hydrogenotrophic methanogenesis increased with temperature. Temperature dependence of MPRs was significantly higher in incubations spiked with H2/CO2 (c. 1.9 eV) compared to incubations spiked with acetate or unamended (c. 0.8 eV). Temperature was not found to shape the total microbial community structure, that rather exhibited a site-specific variability among the studied lakes. However, the methanogenic archaeal community structure was driven by amended methanogenic precursors with a dominance of Methanobacterium in H2/CO2-based incubations and Methanosarcina in acetate-based incubations. We also suggested the importance of acetogenic H2-production outcompeting hydrogenotrohic methanogenesis especially at low temperatures, further supported by homoacetogen proportion in the microcosm communities. The combination of in situ-, and laboratory-based measurements and molecular approaches indicates that the hydrogenotrophic pathway may become more important with increasing temperatures ... |
format |
Article in Journal/Newspaper |
author |
Céline Lavergne Polette Aguilar-Muñoz Natalia Calle Frédéric Thalasso Maria Soledad Astorga-España Armando Sepulveda-Jauregui Karla Martinez-Cruz Laure Gandois Andrés Mansilla Rolando Chamy Maialen Barret Léa Cabrol |
author_facet |
Céline Lavergne Polette Aguilar-Muñoz Natalia Calle Frédéric Thalasso Maria Soledad Astorga-España Armando Sepulveda-Jauregui Karla Martinez-Cruz Laure Gandois Andrés Mansilla Rolando Chamy Maialen Barret Léa Cabrol |
author_sort |
Céline Lavergne |
title |
Temperature differently affected methanogenic pathways and microbial communities in sub-Antarctic freshwater ecosystems |
title_short |
Temperature differently affected methanogenic pathways and microbial communities in sub-Antarctic freshwater ecosystems |
title_full |
Temperature differently affected methanogenic pathways and microbial communities in sub-Antarctic freshwater ecosystems |
title_fullStr |
Temperature differently affected methanogenic pathways and microbial communities in sub-Antarctic freshwater ecosystems |
title_full_unstemmed |
Temperature differently affected methanogenic pathways and microbial communities in sub-Antarctic freshwater ecosystems |
title_sort |
temperature differently affected methanogenic pathways and microbial communities in sub-antarctic freshwater ecosystems |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://doi.org/10.1016/j.envint.2021.106575 https://doaj.org/article/b71a71c57f45468dae689ed88e5165d1 |
long_lat |
ENVELOPE(-135.021,-135.021,61.583,61.583) ENVELOPE(-62.933,-62.933,-64.883,-64.883) |
geographic |
Antarctic Cape Horn Magallanes |
geographic_facet |
Antarctic Cape Horn Magallanes |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Environment International, Vol 154, Iss , Pp 106575- (2021) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S0160412021002002 https://doaj.org/toc/0160-4120 0160-4120 doi:10.1016/j.envint.2021.106575 https://doaj.org/article/b71a71c57f45468dae689ed88e5165d1 |
op_doi |
https://doi.org/10.1016/j.envint.2021.106575 |
container_title |
Environment International |
container_volume |
154 |
container_start_page |
106575 |
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1766239635425460224 |