Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling.
Burkholderia pseudomallei (Bp) causes the disease melioidosis. The main cause of mortality in this disease is septic shock triggered by the host responding to lipopolysaccharide (LPS) components of the Gram-negative outer membrane. Bp LPS is thought to be a weak inducer of the host immune system. LP...
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ftdoajarticles:oai:doaj.org/article:b7097648ecdd4cd2bebfa14ec942af1e 2023-05-15T15:10:40+02:00 Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling. Michael H Norris Herbert P Schweizer Apichai Tuanyok 2017-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005571 https://doaj.org/article/b7097648ecdd4cd2bebfa14ec942af1e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5425228?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005571 https://doaj.org/article/b7097648ecdd4cd2bebfa14ec942af1e PLoS Neglected Tropical Diseases, Vol 11, Iss 4, p e0005571 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005571 2022-12-31T14:37:43Z Burkholderia pseudomallei (Bp) causes the disease melioidosis. The main cause of mortality in this disease is septic shock triggered by the host responding to lipopolysaccharide (LPS) components of the Gram-negative outer membrane. Bp LPS is thought to be a weak inducer of the host immune system. LPS from several strains of Bp were purified and their ability to induce the inflammatory mediators TNF-α and iNOS in murine macrophages at low concentrations was investigated. Innate and adaptive immunity qPCR arrays were used to profile expression patterns of 84 gene targets in response to the different LPS types. Additional qPCR validation confirmed large differences in macrophage response. LPS from a high-virulence serotype B strain 576a and a virulent rough central nervous system tropic strain MSHR435 greatly induced the innate immune response indicating that the immunopathogenesis of these strains is different than in infections with strains similar to the prototype strain 1026b. The accumulation of autophagic vesicles was also increased in macrophages challenged with highly immunogenic Bp LPS. Gene induction and concomitant cytokine secretion profiles of human PBMCs in response to the various LPS were also investigated. MALDI-TOF/TOF was used to probe the lipid A portions of the LPS, indicating substantial structural differences that likely play a role in host response to LPS. These findings add to the evolving knowledge of host-response to bacterial LPS, which can be used to better understand septic shock in melioidosis patients and in the rational design of vaccines. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 4 e0005571 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Michael H Norris Herbert P Schweizer Apichai Tuanyok Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Burkholderia pseudomallei (Bp) causes the disease melioidosis. The main cause of mortality in this disease is septic shock triggered by the host responding to lipopolysaccharide (LPS) components of the Gram-negative outer membrane. Bp LPS is thought to be a weak inducer of the host immune system. LPS from several strains of Bp were purified and their ability to induce the inflammatory mediators TNF-α and iNOS in murine macrophages at low concentrations was investigated. Innate and adaptive immunity qPCR arrays were used to profile expression patterns of 84 gene targets in response to the different LPS types. Additional qPCR validation confirmed large differences in macrophage response. LPS from a high-virulence serotype B strain 576a and a virulent rough central nervous system tropic strain MSHR435 greatly induced the innate immune response indicating that the immunopathogenesis of these strains is different than in infections with strains similar to the prototype strain 1026b. The accumulation of autophagic vesicles was also increased in macrophages challenged with highly immunogenic Bp LPS. Gene induction and concomitant cytokine secretion profiles of human PBMCs in response to the various LPS were also investigated. MALDI-TOF/TOF was used to probe the lipid A portions of the LPS, indicating substantial structural differences that likely play a role in host response to LPS. These findings add to the evolving knowledge of host-response to bacterial LPS, which can be used to better understand septic shock in melioidosis patients and in the rational design of vaccines. |
format |
Article in Journal/Newspaper |
author |
Michael H Norris Herbert P Schweizer Apichai Tuanyok |
author_facet |
Michael H Norris Herbert P Schweizer Apichai Tuanyok |
author_sort |
Michael H Norris |
title |
Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling. |
title_short |
Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling. |
title_full |
Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling. |
title_fullStr |
Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling. |
title_full_unstemmed |
Structural diversity of Burkholderia pseudomallei lipopolysaccharides affects innate immune signaling. |
title_sort |
structural diversity of burkholderia pseudomallei lipopolysaccharides affects innate immune signaling. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005571 https://doaj.org/article/b7097648ecdd4cd2bebfa14ec942af1e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 4, p e0005571 (2017) |
op_relation |
http://europepmc.org/articles/PMC5425228?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005571 https://doaj.org/article/b7097648ecdd4cd2bebfa14ec942af1e |
op_doi |
https://doi.org/10.1371/journal.pntd.0005571 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
4 |
container_start_page |
e0005571 |
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1766341652454047744 |