Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii.
Background Pathogens can impact host RNA modification machinery to establish a favorable cellular environment for their replication. In the present study, we investigated the effect of Toxoplasma gondii infection on host RNA modification profiles and explored how these modifications may influence th...
Published in: | PLOS Neglected Tropical Diseases |
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Online Access: | https://doi.org/10.1371/journal.pntd.0012281 https://doaj.org/article/b6e94ac4455f4fe2beb2702602f4a7c3 |
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ftdoajarticles:oai:doaj.org/article:b6e94ac4455f4fe2beb2702602f4a7c3 2024-09-09T19:25:55+00:00 Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii. Xiao-Xuan Zhang Yu-Zhe Sun Wei Wang Yang Gao Xin-Yu Wei Hong-Chao Sun Chun-Ren Wang Hong-Bo Ni Xing Yang Hany M Elsheikha Huan-Ping Guo 2024-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0012281 https://doaj.org/article/b6e94ac4455f4fe2beb2702602f4a7c3 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0012281 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0012281 https://doaj.org/article/b6e94ac4455f4fe2beb2702602f4a7c3 PLoS Neglected Tropical Diseases, Vol 18, Iss 6, p e0012281 (2024) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2024 ftdoajarticles https://doi.org/10.1371/journal.pntd.0012281 2024-08-05T17:48:59Z Background Pathogens can impact host RNA modification machinery to establish a favorable cellular environment for their replication. In the present study, we investigated the effect of Toxoplasma gondii infection on host RNA modification profiles and explored how these modifications may influence the host-parasite interaction. Methodology/principal findings We analyzed the modification levels of ∼ 80 nt tRNA and 17-50 nt sncRNAs in mouse liver, spleen, and serum using liquid chromatography and tandem mass spectrometry analysis. The results revealed alterations in RNA modification profiles, particularly during acute infection. The liver exhibited more differentially abundant RNA modifications than the spleen. RNA modification levels in serum were mostly downregulated during acute infection compared to control mice. Correlations were detected between different RNA modifications in the liver and spleen during infection and between several RNA modifications and many cytokines. Alterations in RNA modifications affected tRNA stability and protein translation. Conclusions/significance These findings provide new insight into the role of RNA modifications in mediating the murine host response to T. gondii infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 18 6 e0012281 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Xiao-Xuan Zhang Yu-Zhe Sun Wei Wang Yang Gao Xin-Yu Wei Hong-Chao Sun Chun-Ren Wang Hong-Bo Ni Xing Yang Hany M Elsheikha Huan-Ping Guo Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Pathogens can impact host RNA modification machinery to establish a favorable cellular environment for their replication. In the present study, we investigated the effect of Toxoplasma gondii infection on host RNA modification profiles and explored how these modifications may influence the host-parasite interaction. Methodology/principal findings We analyzed the modification levels of ∼ 80 nt tRNA and 17-50 nt sncRNAs in mouse liver, spleen, and serum using liquid chromatography and tandem mass spectrometry analysis. The results revealed alterations in RNA modification profiles, particularly during acute infection. The liver exhibited more differentially abundant RNA modifications than the spleen. RNA modification levels in serum were mostly downregulated during acute infection compared to control mice. Correlations were detected between different RNA modifications in the liver and spleen during infection and between several RNA modifications and many cytokines. Alterations in RNA modifications affected tRNA stability and protein translation. Conclusions/significance These findings provide new insight into the role of RNA modifications in mediating the murine host response to T. gondii infection. |
format |
Article in Journal/Newspaper |
author |
Xiao-Xuan Zhang Yu-Zhe Sun Wei Wang Yang Gao Xin-Yu Wei Hong-Chao Sun Chun-Ren Wang Hong-Bo Ni Xing Yang Hany M Elsheikha Huan-Ping Guo |
author_facet |
Xiao-Xuan Zhang Yu-Zhe Sun Wei Wang Yang Gao Xin-Yu Wei Hong-Chao Sun Chun-Ren Wang Hong-Bo Ni Xing Yang Hany M Elsheikha Huan-Ping Guo |
author_sort |
Xiao-Xuan Zhang |
title |
Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii. |
title_short |
Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii. |
title_full |
Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii. |
title_fullStr |
Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii. |
title_full_unstemmed |
Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii. |
title_sort |
altered landscape of total rna, trna and sncrna modifications in the liver and spleen of mice infected by toxoplasma gondii. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2024 |
url |
https://doi.org/10.1371/journal.pntd.0012281 https://doaj.org/article/b6e94ac4455f4fe2beb2702602f4a7c3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 18, Iss 6, p e0012281 (2024) |
op_relation |
https://doi.org/10.1371/journal.pntd.0012281 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0012281 https://doaj.org/article/b6e94ac4455f4fe2beb2702602f4a7c3 |
op_doi |
https://doi.org/10.1371/journal.pntd.0012281 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
18 |
container_issue |
6 |
container_start_page |
e0012281 |
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1809895620115169280 |