Preparation and in vitro characterization of chitosan nanoparticles containing Mesobuthus eupeus scorpion venom as an antigen delivery system
Hydrophilic nanoparticles have been widely investigated in recent years as delivery systems for therapeutic macromolecules such as antigens. In the present study Mesobuthus eupeus venom-loaded chitosan nanoparticles were prepared via ionic gelation of tripolyphosphate (TPP) and chitosan. The optimum...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:b6d07c8fdf2d455984f27a1ffe0f0853 2023-05-15T15:07:16+02:00 Preparation and in vitro characterization of chitosan nanoparticles containing Mesobuthus eupeus scorpion venom as an antigen delivery system N Mohammadpour Dounighi R Eskandari MR Avadi H Zolfagharian A Mir Mohammad Sadeghi M Rezayat 2012-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992012000100006 https://doaj.org/article/b6d07c8fdf2d455984f27a1ffe0f0853 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100006 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000100006 1678-9199 https://doaj.org/article/b6d07c8fdf2d455984f27a1ffe0f0853 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 1, Pp 44-52 (2012) nanoparticle chitosan tripolyphosphate venom antigen delivery system Mesobuthus eupeus Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2012 ftdoajarticles https://doi.org/10.1590/S1678-91992012000100006 2022-12-31T14:28:51Z Hydrophilic nanoparticles have been widely investigated in recent years as delivery systems for therapeutic macromolecules such as antigens. In the present study Mesobuthus eupeus venom-loaded chitosan nanoparticles were prepared via ionic gelation of tripolyphosphate (TPP) and chitosan. The optimum encapsulation efficiency (91.1%) and loading capacity (76.3%) were obtained by a chitosan concentration of 2 mg/mL, chitosan-to-TPP mass ratio of 2 and M. eupeus venom concentration of 500 µg/mL. The average nanoparticle size at optimum conditions was determined by Zetasizer (Malvern Instruments, UK). The nanoparticle size was about 370 nm (polydispersity index: 0.429) while the zeta potential was positive. Transmission electron microscope (TEM) imaging showed a spherical, smooth and almost homogenous structure for nanoparticles. Fourier transform infrared (FTIR) spectroscopy confirmed tripolyphosphoric groups of TPP linked with ammonium groups of chitosan in the nanoparticles. The in vitro release of nanoparticles showed an initial burst release of approximately 60% in the first ten hours, followed by a slow and much reduced additional release for about 60 hours. It is suggested that the chitosan nanoparticles fabricated in our study may provide a suitable alternative to traditional adjuvant systems. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 18 1 44 52 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
nanoparticle chitosan tripolyphosphate venom antigen delivery system Mesobuthus eupeus Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
nanoparticle chitosan tripolyphosphate venom antigen delivery system Mesobuthus eupeus Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 N Mohammadpour Dounighi R Eskandari MR Avadi H Zolfagharian A Mir Mohammad Sadeghi M Rezayat Preparation and in vitro characterization of chitosan nanoparticles containing Mesobuthus eupeus scorpion venom as an antigen delivery system |
topic_facet |
nanoparticle chitosan tripolyphosphate venom antigen delivery system Mesobuthus eupeus Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Hydrophilic nanoparticles have been widely investigated in recent years as delivery systems for therapeutic macromolecules such as antigens. In the present study Mesobuthus eupeus venom-loaded chitosan nanoparticles were prepared via ionic gelation of tripolyphosphate (TPP) and chitosan. The optimum encapsulation efficiency (91.1%) and loading capacity (76.3%) were obtained by a chitosan concentration of 2 mg/mL, chitosan-to-TPP mass ratio of 2 and M. eupeus venom concentration of 500 µg/mL. The average nanoparticle size at optimum conditions was determined by Zetasizer (Malvern Instruments, UK). The nanoparticle size was about 370 nm (polydispersity index: 0.429) while the zeta potential was positive. Transmission electron microscope (TEM) imaging showed a spherical, smooth and almost homogenous structure for nanoparticles. Fourier transform infrared (FTIR) spectroscopy confirmed tripolyphosphoric groups of TPP linked with ammonium groups of chitosan in the nanoparticles. The in vitro release of nanoparticles showed an initial burst release of approximately 60% in the first ten hours, followed by a slow and much reduced additional release for about 60 hours. It is suggested that the chitosan nanoparticles fabricated in our study may provide a suitable alternative to traditional adjuvant systems. |
format |
Article in Journal/Newspaper |
author |
N Mohammadpour Dounighi R Eskandari MR Avadi H Zolfagharian A Mir Mohammad Sadeghi M Rezayat |
author_facet |
N Mohammadpour Dounighi R Eskandari MR Avadi H Zolfagharian A Mir Mohammad Sadeghi M Rezayat |
author_sort |
N Mohammadpour Dounighi |
title |
Preparation and in vitro characterization of chitosan nanoparticles containing Mesobuthus eupeus scorpion venom as an antigen delivery system |
title_short |
Preparation and in vitro characterization of chitosan nanoparticles containing Mesobuthus eupeus scorpion venom as an antigen delivery system |
title_full |
Preparation and in vitro characterization of chitosan nanoparticles containing Mesobuthus eupeus scorpion venom as an antigen delivery system |
title_fullStr |
Preparation and in vitro characterization of chitosan nanoparticles containing Mesobuthus eupeus scorpion venom as an antigen delivery system |
title_full_unstemmed |
Preparation and in vitro characterization of chitosan nanoparticles containing Mesobuthus eupeus scorpion venom as an antigen delivery system |
title_sort |
preparation and in vitro characterization of chitosan nanoparticles containing mesobuthus eupeus scorpion venom as an antigen delivery system |
publisher |
SciELO |
publishDate |
2012 |
url |
https://doi.org/10.1590/S1678-91992012000100006 https://doaj.org/article/b6d07c8fdf2d455984f27a1ffe0f0853 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 18, Iss 1, Pp 44-52 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000100006 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992012000100006 1678-9199 https://doaj.org/article/b6d07c8fdf2d455984f27a1ffe0f0853 |
op_doi |
https://doi.org/10.1590/S1678-91992012000100006 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
18 |
container_issue |
1 |
container_start_page |
44 |
op_container_end_page |
52 |
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1766338802878513152 |