Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp.
Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter , were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The...
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ftdoajarticles:oai:doaj.org/article:b6b5882bd1594551a3af4dc63c4e8200 2023-05-15T15:38:54+02:00 Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. Yannik K.-H. Schneider Kine Ø. Hansen Johan Isaksson Sara Ullsten Espen H. Hansen Jeanette Hammer Andersen 2019-11-01T00:00:00Z https://doi.org/10.3390/molecules24213991 https://doaj.org/article/b6b5882bd1594551a3af4dc63c4e8200 EN eng MDPI AG https://www.mdpi.com/1420-3049/24/21/3991 https://doaj.org/toc/1420-3049 1420-3049 doi:10.3390/molecules24213991 https://doaj.org/article/b6b5882bd1594551a3af4dc63c4e8200 Molecules, Vol 24, Iss 21, p 3991 (2019) flavolipin marine bacteria natural products lipopeptides algibacter Organic chemistry QD241-441 article 2019 ftdoajarticles https://doi.org/10.3390/molecules24213991 2022-12-31T02:29:18Z Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter , were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The structure of the bioactive compound was identified via mass spectrometry and nuclear resonance spectroscopy and it turned out to be the known lipopeptide Lipid 430. We discovered and determined its previously unknown anti-bacterial activity against Streptococcus agalactiae and revealed a cytotoxic effect against the A2058 human melanoma cell line at significantly lower concentrations compared to its anti-bacterial concentration. Flow cytometry and microscopy investigations of the cytotoxicity against the melanoma cell line indicated that Lipid 430 did not cause immediate cell lysis. The experiments with melanoma cells suggest that the compound functions trough more complex pathways than acting as a simple detergent. Article in Journal/Newspaper Barents Sea Directory of Open Access Journals: DOAJ Articles Barents Sea Molecules 24 21 3991 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
flavolipin marine bacteria natural products lipopeptides algibacter Organic chemistry QD241-441 |
spellingShingle |
flavolipin marine bacteria natural products lipopeptides algibacter Organic chemistry QD241-441 Yannik K.-H. Schneider Kine Ø. Hansen Johan Isaksson Sara Ullsten Espen H. Hansen Jeanette Hammer Andersen Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
topic_facet |
flavolipin marine bacteria natural products lipopeptides algibacter Organic chemistry QD241-441 |
description |
Two bacterial isolates from the Barents Sea, both belonging to the genus Algibacter , were found to yield extracts with anti-bacterial bioactivity. Mass spectrometry guided dereplication and purification of the active extracts lead to the isolation of the same active principle in both extracts. The structure of the bioactive compound was identified via mass spectrometry and nuclear resonance spectroscopy and it turned out to be the known lipopeptide Lipid 430. We discovered and determined its previously unknown anti-bacterial activity against Streptococcus agalactiae and revealed a cytotoxic effect against the A2058 human melanoma cell line at significantly lower concentrations compared to its anti-bacterial concentration. Flow cytometry and microscopy investigations of the cytotoxicity against the melanoma cell line indicated that Lipid 430 did not cause immediate cell lysis. The experiments with melanoma cells suggest that the compound functions trough more complex pathways than acting as a simple detergent. |
format |
Article in Journal/Newspaper |
author |
Yannik K.-H. Schneider Kine Ø. Hansen Johan Isaksson Sara Ullsten Espen H. Hansen Jeanette Hammer Andersen |
author_facet |
Yannik K.-H. Schneider Kine Ø. Hansen Johan Isaksson Sara Ullsten Espen H. Hansen Jeanette Hammer Andersen |
author_sort |
Yannik K.-H. Schneider |
title |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_short |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_full |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_fullStr |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_full_unstemmed |
Anti-Bacterial Effect and Cytotoxicity Assessment of Lipid 430 Isolated from Algibacter sp. |
title_sort |
anti-bacterial effect and cytotoxicity assessment of lipid 430 isolated from algibacter sp. |
publisher |
MDPI AG |
publishDate |
2019 |
url |
https://doi.org/10.3390/molecules24213991 https://doaj.org/article/b6b5882bd1594551a3af4dc63c4e8200 |
geographic |
Barents Sea |
geographic_facet |
Barents Sea |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_source |
Molecules, Vol 24, Iss 21, p 3991 (2019) |
op_relation |
https://www.mdpi.com/1420-3049/24/21/3991 https://doaj.org/toc/1420-3049 1420-3049 doi:10.3390/molecules24213991 https://doaj.org/article/b6b5882bd1594551a3af4dc63c4e8200 |
op_doi |
https://doi.org/10.3390/molecules24213991 |
container_title |
Molecules |
container_volume |
24 |
container_issue |
21 |
container_start_page |
3991 |
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1766370319505817600 |