Emprego do antígeno tríplice, em fixação do complemento em placa para exclusão sorológica da moléstia de Chagas, Sífilis e Brucelose

• Main Author: Therezinha Aparecida Cunha
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 1976
• Subjects: Arctic medicine. Tropical medicine; RC955-962; Arctic; Elevado
• Online Access: https://doaj.org/article/b6b505dcabd74a209a91138e2b551d70

O método de fixação de complemento em gotas, sobre placas de plástico, usando antígeno misto de cardiolipina, suspensão de brucelas e extrato de T. cruzi, foi utilizado no exame de 112.365 soros provenientes de bancos de sangue e do Hospital das Clínicas de Ribeirão Preto. Os 18.279 soros que reagiram com o antígeno tríplice foram re-examinados com cada um dos antígenos, verificando-se que 77% a 88% deles eram chagásicos, de 8% a 23% eram sifilíticos e que 1% a 3% reagiam com o antígeno de brucelas. Pela sua simplicidade, economia de tempo e de material, a técnica de fixação de complemento, com antígeno tríplice, se recomenda para a rotina dos bancos de sangue, hospitais e laboratórios de saúde pública, quando número elevado de sangue deve ser examinado. The complement-fixation in drops, using a triple antigen, was employed for screening out sera from Chagas' disease, syphilis or brucellosis. A total of 112, 365 sera were tested, from which 18,279 reacted with the triple antigen (composed of cardiolipin, suspension of brucellas and T. cruzi antigen). Alt the reactive sera were retested with each one of the antigens, in order to identify the infection. Chagas' disease was found in the proportion of 77% to 88% in this group, syphilis from 8% to 23% and 1% to 3% reacted with brucella antigen. The use of a triple antigen in the complement-fixation test in drops has been very suitable for screening out reacting sera for Chagas' disease, syphilis and brucellosis, not only for the selection of blood donors, but also for the routine of serology in public health laboratories and hospitais, when large number of sera are to be tested.