An "in-depth" description of the small non-coding RNA population of Schistosoma japonicum schistosomulum.
Parasitic flatworms of the genus Schistosoma are the causative agents of schistosomiasis, which afflicts more than 200 million people yearly in tropical regions of South America, Asia and Africa. A promising approach to the control of this and many other diseases involves the application of our unde...
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ftdoajarticles:oai:doaj.org/article:b6b21168bc3a4a548291bc080e024246 2023-05-15T15:11:07+02:00 An "in-depth" description of the small non-coding RNA population of Schistosoma japonicum schistosomulum. Zhangxun Wang Xiangyang Xue Jun Sun Rong Luo Xindong Xu Yanyan Jiang Qingfeng Zhang Weiqing Pan 2010-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000596 https://doaj.org/article/b6b21168bc3a4a548291bc080e024246 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2817716?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000596 https://doaj.org/article/b6b21168bc3a4a548291bc080e024246 PLoS Neglected Tropical Diseases, Vol 4, Iss 2, p e596 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000596 2022-12-31T07:22:37Z Parasitic flatworms of the genus Schistosoma are the causative agents of schistosomiasis, which afflicts more than 200 million people yearly in tropical regions of South America, Asia and Africa. A promising approach to the control of this and many other diseases involves the application of our understanding of small non-coding RNA function to the design of safe and effective means of treatment. In a previous study, we identified five conserved miRNAs from the adult stage of Schistosoma japonicum. Here, we applied Illumina Solexa high-throughput sequencing methods (deep sequencing) to investigate the small RNAs expressed in S. japonicum schistosomulum (3 weeks post-infection). This has allowed us to examine over four million sequence reads including both frequently and infrequently represented members of the RNA population. Thus we have identified 20 conserved miRNA families that have orthologs in well-studied model organisms and 16 miRNA that appear to be specific to Schistosoma. We have also observed minor amounts of heterogeneity in both 3' and 5' terminal positions of some miRNA as well as RNA fragments resulting from the processing of miRNA precursor. An investigation of the genomic arrangement of the 36 identified miRNA revealed that seven were tightly linked in two clusters. We also identified members of the small RNA population whose structure indicates that they are part of an endogenously derived RNA silencing pathway, as evidenced by their extensive complementarities with retrotransposon and retrovirus-related Pol polyprotein from transposon. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 2 e596 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Zhangxun Wang Xiangyang Xue Jun Sun Rong Luo Xindong Xu Yanyan Jiang Qingfeng Zhang Weiqing Pan An "in-depth" description of the small non-coding RNA population of Schistosoma japonicum schistosomulum. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Parasitic flatworms of the genus Schistosoma are the causative agents of schistosomiasis, which afflicts more than 200 million people yearly in tropical regions of South America, Asia and Africa. A promising approach to the control of this and many other diseases involves the application of our understanding of small non-coding RNA function to the design of safe and effective means of treatment. In a previous study, we identified five conserved miRNAs from the adult stage of Schistosoma japonicum. Here, we applied Illumina Solexa high-throughput sequencing methods (deep sequencing) to investigate the small RNAs expressed in S. japonicum schistosomulum (3 weeks post-infection). This has allowed us to examine over four million sequence reads including both frequently and infrequently represented members of the RNA population. Thus we have identified 20 conserved miRNA families that have orthologs in well-studied model organisms and 16 miRNA that appear to be specific to Schistosoma. We have also observed minor amounts of heterogeneity in both 3' and 5' terminal positions of some miRNA as well as RNA fragments resulting from the processing of miRNA precursor. An investigation of the genomic arrangement of the 36 identified miRNA revealed that seven were tightly linked in two clusters. We also identified members of the small RNA population whose structure indicates that they are part of an endogenously derived RNA silencing pathway, as evidenced by their extensive complementarities with retrotransposon and retrovirus-related Pol polyprotein from transposon. |
format |
Article in Journal/Newspaper |
author |
Zhangxun Wang Xiangyang Xue Jun Sun Rong Luo Xindong Xu Yanyan Jiang Qingfeng Zhang Weiqing Pan |
author_facet |
Zhangxun Wang Xiangyang Xue Jun Sun Rong Luo Xindong Xu Yanyan Jiang Qingfeng Zhang Weiqing Pan |
author_sort |
Zhangxun Wang |
title |
An "in-depth" description of the small non-coding RNA population of Schistosoma japonicum schistosomulum. |
title_short |
An "in-depth" description of the small non-coding RNA population of Schistosoma japonicum schistosomulum. |
title_full |
An "in-depth" description of the small non-coding RNA population of Schistosoma japonicum schistosomulum. |
title_fullStr |
An "in-depth" description of the small non-coding RNA population of Schistosoma japonicum schistosomulum. |
title_full_unstemmed |
An "in-depth" description of the small non-coding RNA population of Schistosoma japonicum schistosomulum. |
title_sort |
"in-depth" description of the small non-coding rna population of schistosoma japonicum schistosomulum. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000596 https://doaj.org/article/b6b21168bc3a4a548291bc080e024246 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 2, p e596 (2010) |
op_relation |
http://europepmc.org/articles/PMC2817716?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000596 https://doaj.org/article/b6b21168bc3a4a548291bc080e024246 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000596 |
container_title |
PLoS Neglected Tropical Diseases |
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4 |
container_issue |
2 |
container_start_page |
e596 |
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1766342030297923584 |