Severe Fever with Thrombocytopenia Syndrome Virus Antigen Detection Using Monoclonal Antibodies to the Nucleocapsid Protein.
Severe fever with thrombocytopenia syndrome (SFTS) is a tick-borne infectious disease with a high case fatality rate, and is caused by the SFTS virus (SFTSV). SFTS is endemic to China, South Korea, and Japan. The viral RNA level in sera of patients with SFTS is known to be strongly associated with o...
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ftdoajarticles:oai:doaj.org/article:b6b0aad851e34abcb720b256ad5c3fd7 2023-05-15T15:11:08+02:00 Severe Fever with Thrombocytopenia Syndrome Virus Antigen Detection Using Monoclonal Antibodies to the Nucleocapsid Protein. Aiko Fukuma Shuetsu Fukushi Tomoki Yoshikawa Hideki Tani Satoshi Taniguchi Takeshi Kurosu Kazutaka Egawa Yuto Suda Harpal Singh Taro Nomachi Mutsuyo Gokuden Katsuyuki Ando Kouji Kida Miki Kan Nobuyuki Kato Akira Yoshikawa Hiroaki Kitamoto Yuko Sato Tadaki Suzuki Hideki Hasegawa Shigeru Morikawa Masayuki Shimojima Masayuki Saijo 2016-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004595 https://doaj.org/article/b6b0aad851e34abcb720b256ad5c3fd7 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4821557?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004595 https://doaj.org/article/b6b0aad851e34abcb720b256ad5c3fd7 PLoS Neglected Tropical Diseases, Vol 10, Iss 4, p e0004595 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004595 2022-12-31T05:44:55Z Severe fever with thrombocytopenia syndrome (SFTS) is a tick-borne infectious disease with a high case fatality rate, and is caused by the SFTS virus (SFTSV). SFTS is endemic to China, South Korea, and Japan. The viral RNA level in sera of patients with SFTS is known to be strongly associated with outcomes. Virological SFTS diagnosis with high sensitivity and specificity are required in disease endemic areas.We generated novel monoclonal antibodies (MAbs) against the SFTSV nucleocapsid (N) protein and developed a sandwich antigen (Ag)-capture enzyme-linked immunosorbent assay (ELISA) for the detection of N protein of SFTSV using MAb and polyclonal antibody as capture and detection antibodies, respectively. The Ag-capture system was capable of detecting at least 350-1220 TCID50/100 μl/well from the culture supernatants of various SFTSV strains. The efficacy of the Ag-capture ELISA in SFTS diagnosis was evaluated using serum samples collected from patients suspected of having SFTS in Japan. All 24 serum samples (100%) containing high copy numbers of viral RNA (>105 copies/ml) showed a positive reaction in the Ag-capture ELISA, whereas 12 out of 15 serum samples (80%) containing low copy numbers of viral RNA (<105 copies/ml) showed a negative reaction in the Ag-capture ELISA. Among these Ag-capture ELISA-negative 12 samples, 9 (75%) were positive for IgG antibodies against SFTSV.The newly developed Ag-capture ELISA is useful for SFTS diagnosis in acute phase patients with high levels of viremia. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 4 e0004595 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Aiko Fukuma Shuetsu Fukushi Tomoki Yoshikawa Hideki Tani Satoshi Taniguchi Takeshi Kurosu Kazutaka Egawa Yuto Suda Harpal Singh Taro Nomachi Mutsuyo Gokuden Katsuyuki Ando Kouji Kida Miki Kan Nobuyuki Kato Akira Yoshikawa Hiroaki Kitamoto Yuko Sato Tadaki Suzuki Hideki Hasegawa Shigeru Morikawa Masayuki Shimojima Masayuki Saijo Severe Fever with Thrombocytopenia Syndrome Virus Antigen Detection Using Monoclonal Antibodies to the Nucleocapsid Protein. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Severe fever with thrombocytopenia syndrome (SFTS) is a tick-borne infectious disease with a high case fatality rate, and is caused by the SFTS virus (SFTSV). SFTS is endemic to China, South Korea, and Japan. The viral RNA level in sera of patients with SFTS is known to be strongly associated with outcomes. Virological SFTS diagnosis with high sensitivity and specificity are required in disease endemic areas.We generated novel monoclonal antibodies (MAbs) against the SFTSV nucleocapsid (N) protein and developed a sandwich antigen (Ag)-capture enzyme-linked immunosorbent assay (ELISA) for the detection of N protein of SFTSV using MAb and polyclonal antibody as capture and detection antibodies, respectively. The Ag-capture system was capable of detecting at least 350-1220 TCID50/100 μl/well from the culture supernatants of various SFTSV strains. The efficacy of the Ag-capture ELISA in SFTS diagnosis was evaluated using serum samples collected from patients suspected of having SFTS in Japan. All 24 serum samples (100%) containing high copy numbers of viral RNA (>105 copies/ml) showed a positive reaction in the Ag-capture ELISA, whereas 12 out of 15 serum samples (80%) containing low copy numbers of viral RNA (<105 copies/ml) showed a negative reaction in the Ag-capture ELISA. Among these Ag-capture ELISA-negative 12 samples, 9 (75%) were positive for IgG antibodies against SFTSV.The newly developed Ag-capture ELISA is useful for SFTS diagnosis in acute phase patients with high levels of viremia. |
format |
Article in Journal/Newspaper |
author |
Aiko Fukuma Shuetsu Fukushi Tomoki Yoshikawa Hideki Tani Satoshi Taniguchi Takeshi Kurosu Kazutaka Egawa Yuto Suda Harpal Singh Taro Nomachi Mutsuyo Gokuden Katsuyuki Ando Kouji Kida Miki Kan Nobuyuki Kato Akira Yoshikawa Hiroaki Kitamoto Yuko Sato Tadaki Suzuki Hideki Hasegawa Shigeru Morikawa Masayuki Shimojima Masayuki Saijo |
author_facet |
Aiko Fukuma Shuetsu Fukushi Tomoki Yoshikawa Hideki Tani Satoshi Taniguchi Takeshi Kurosu Kazutaka Egawa Yuto Suda Harpal Singh Taro Nomachi Mutsuyo Gokuden Katsuyuki Ando Kouji Kida Miki Kan Nobuyuki Kato Akira Yoshikawa Hiroaki Kitamoto Yuko Sato Tadaki Suzuki Hideki Hasegawa Shigeru Morikawa Masayuki Shimojima Masayuki Saijo |
author_sort |
Aiko Fukuma |
title |
Severe Fever with Thrombocytopenia Syndrome Virus Antigen Detection Using Monoclonal Antibodies to the Nucleocapsid Protein. |
title_short |
Severe Fever with Thrombocytopenia Syndrome Virus Antigen Detection Using Monoclonal Antibodies to the Nucleocapsid Protein. |
title_full |
Severe Fever with Thrombocytopenia Syndrome Virus Antigen Detection Using Monoclonal Antibodies to the Nucleocapsid Protein. |
title_fullStr |
Severe Fever with Thrombocytopenia Syndrome Virus Antigen Detection Using Monoclonal Antibodies to the Nucleocapsid Protein. |
title_full_unstemmed |
Severe Fever with Thrombocytopenia Syndrome Virus Antigen Detection Using Monoclonal Antibodies to the Nucleocapsid Protein. |
title_sort |
severe fever with thrombocytopenia syndrome virus antigen detection using monoclonal antibodies to the nucleocapsid protein. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004595 https://doaj.org/article/b6b0aad851e34abcb720b256ad5c3fd7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 4, p e0004595 (2016) |
op_relation |
http://europepmc.org/articles/PMC4821557?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004595 https://doaj.org/article/b6b0aad851e34abcb720b256ad5c3fd7 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004595 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
4 |
container_start_page |
e0004595 |
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1766342044670754816 |