Prevalence and Genotype of Trichomonas vaginalis among Men in Xinxiang City, Henan Province, China
Trichomonas vaginalis (T. vaginalis) could cause trichomoniasis through sexual transmission, which was globally distributed. In this study, the prevalence and phylogenetic analyses of T. vaginalis among men in Xinxiang were conducted. From October 2018 to December 2019, a total of 634 male clinical...
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ftdoajarticles:oai:doaj.org/article:b6aa2cdd476943beb7c2790487fb5394 2024-09-09T19:26:17+00:00 Prevalence and Genotype of Trichomonas vaginalis among Men in Xinxiang City, Henan Province, China Zhenchao Zhang Yuhui Sang Pucheng Wu Yujia Shang Lesong Li Yujuan Duan Linfei Zhao Minghui Gao Lihua Guo Xiaowei Tian Zhenke Yang Shuai Wang Lixia Hao Xuefang Mei 2023-01-01T00:00:00Z https://doi.org/10.1155/2023/4119956 https://doaj.org/article/b6aa2cdd476943beb7c2790487fb5394 EN eng Wiley http://dx.doi.org/10.1155/2023/4119956 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2023/4119956 https://doaj.org/article/b6aa2cdd476943beb7c2790487fb5394 Journal of Tropical Medicine, Vol 2023 (2023) Arctic medicine. Tropical medicine RC955-962 article 2023 ftdoajarticles https://doi.org/10.1155/2023/4119956 2024-08-05T17:48:32Z Trichomonas vaginalis (T. vaginalis) could cause trichomoniasis through sexual transmission, which was globally distributed. In this study, the prevalence and phylogenetic analyses of T. vaginalis among men in Xinxiang were conducted. From October 2018 to December 2019, a total of 634 male clinical samples were collected, including 254 samples of semen, 43 samples of prostate fluid, and 337 samples of urine. These samples were examined by nested PCR and a total of 32 (5.05%) T. vaginalis-positive samples were detected. Among these samples, the positive rates of T. vaginalis in semen, prostate fluid, and urine were 7.87% (20/254), 4.65% (2/43), and 2.97% (10/337), respectively. Three actin genes were successfully isolated and sequenced from the 32 positive DNA samples, and the analysis of the sequence and phylogenetic tree showed that the three actin gene sequences exhibited 99.7%–100% homology to the published actin gene sequence (EU076580) in NCBI, and the T. vaginalis strains in the three positive samples were identified as genotype E. Our results demonstrate a notable genotype of T. vaginalis in the male population and provide insight into the performance of these genetic markers in the molecular epidemiology of trichomoniasis. However, further studies are needed to research the association between the genotype and the pathogenicity of T. vaginalis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2023 1 7 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Zhenchao Zhang Yuhui Sang Pucheng Wu Yujia Shang Lesong Li Yujuan Duan Linfei Zhao Minghui Gao Lihua Guo Xiaowei Tian Zhenke Yang Shuai Wang Lixia Hao Xuefang Mei Prevalence and Genotype of Trichomonas vaginalis among Men in Xinxiang City, Henan Province, China |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
Trichomonas vaginalis (T. vaginalis) could cause trichomoniasis through sexual transmission, which was globally distributed. In this study, the prevalence and phylogenetic analyses of T. vaginalis among men in Xinxiang were conducted. From October 2018 to December 2019, a total of 634 male clinical samples were collected, including 254 samples of semen, 43 samples of prostate fluid, and 337 samples of urine. These samples were examined by nested PCR and a total of 32 (5.05%) T. vaginalis-positive samples were detected. Among these samples, the positive rates of T. vaginalis in semen, prostate fluid, and urine were 7.87% (20/254), 4.65% (2/43), and 2.97% (10/337), respectively. Three actin genes were successfully isolated and sequenced from the 32 positive DNA samples, and the analysis of the sequence and phylogenetic tree showed that the three actin gene sequences exhibited 99.7%–100% homology to the published actin gene sequence (EU076580) in NCBI, and the T. vaginalis strains in the three positive samples were identified as genotype E. Our results demonstrate a notable genotype of T. vaginalis in the male population and provide insight into the performance of these genetic markers in the molecular epidemiology of trichomoniasis. However, further studies are needed to research the association between the genotype and the pathogenicity of T. vaginalis. |
format |
Article in Journal/Newspaper |
author |
Zhenchao Zhang Yuhui Sang Pucheng Wu Yujia Shang Lesong Li Yujuan Duan Linfei Zhao Minghui Gao Lihua Guo Xiaowei Tian Zhenke Yang Shuai Wang Lixia Hao Xuefang Mei |
author_facet |
Zhenchao Zhang Yuhui Sang Pucheng Wu Yujia Shang Lesong Li Yujuan Duan Linfei Zhao Minghui Gao Lihua Guo Xiaowei Tian Zhenke Yang Shuai Wang Lixia Hao Xuefang Mei |
author_sort |
Zhenchao Zhang |
title |
Prevalence and Genotype of Trichomonas vaginalis among Men in Xinxiang City, Henan Province, China |
title_short |
Prevalence and Genotype of Trichomonas vaginalis among Men in Xinxiang City, Henan Province, China |
title_full |
Prevalence and Genotype of Trichomonas vaginalis among Men in Xinxiang City, Henan Province, China |
title_fullStr |
Prevalence and Genotype of Trichomonas vaginalis among Men in Xinxiang City, Henan Province, China |
title_full_unstemmed |
Prevalence and Genotype of Trichomonas vaginalis among Men in Xinxiang City, Henan Province, China |
title_sort |
prevalence and genotype of trichomonas vaginalis among men in xinxiang city, henan province, china |
publisher |
Wiley |
publishDate |
2023 |
url |
https://doi.org/10.1155/2023/4119956 https://doaj.org/article/b6aa2cdd476943beb7c2790487fb5394 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2023 (2023) |
op_relation |
http://dx.doi.org/10.1155/2023/4119956 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2023/4119956 https://doaj.org/article/b6aa2cdd476943beb7c2790487fb5394 |
op_doi |
https://doi.org/10.1155/2023/4119956 |
container_title |
Journal of Tropical Medicine |
container_volume |
2023 |
container_start_page |
1 |
op_container_end_page |
7 |
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1809895934982619136 |