Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod
Lipid metabolism is essential in maintaining energy homeostasis in multicellular organisms. In vertebrates, the peroxisome proliferator-activated receptors (PPARs, NR1C) regulate the expression of many genes involved in these processes. Atlantic cod (Gadus morhua) is an important fish species in the...
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2023
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ftdoajarticles:oai:doaj.org/article:b68b5da6a8394e70bf75e8248ce668ce 2023-05-15T15:26:44+02:00 Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod Marta Eide Anders Goksøyr Fekadu Yadetie Alejandra Gilabert Zdenka Bartosova Håvard G. Frøysa Shirin Fallahi Xiaokang Zhang Nello Blaser Inge Jonassen Per Bruheim Guttorm Alendal Morten Brun Cinta Porte Odd André Karlsen 2023-03-01T00:00:00Z https://doi.org/10.3389/fphys.2023.1129089 https://doaj.org/article/b68b5da6a8394e70bf75e8248ce668ce EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fphys.2023.1129089/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2023.1129089 https://doaj.org/article/b68b5da6a8394e70bf75e8248ce668ce Frontiers in Physiology, Vol 14 (2023) fish lipidomics transcriptomics proteomics WY-14,643 GW501516 Physiology QP1-981 article 2023 ftdoajarticles https://doi.org/10.3389/fphys.2023.1129089 2023-03-26T01:34:33Z Lipid metabolism is essential in maintaining energy homeostasis in multicellular organisms. In vertebrates, the peroxisome proliferator-activated receptors (PPARs, NR1C) regulate the expression of many genes involved in these processes. Atlantic cod (Gadus morhua) is an important fish species in the North Atlantic ecosystem and in human nutrition, with a highly fatty liver. Here we study the involvement of Atlantic cod Ppar a and b subtypes in systemic regulation of lipid metabolism using two model agonists after in vivo exposure. WY-14,643, a specific PPARA ligand in mammals, activated cod Ppara1 and Ppara2 in vitro. In vivo, WY-14,643 caused a shift in lipid transport both at transcriptional and translational level in cod. However, WY-14,643 induced fewer genes in the fatty acid beta-oxidation pathway compared to that observed in rodents. Although GW501516 serves as a specific PPARB/D ligand in mammals, this compound activated cod Ppara1 and Ppara2 as well as Pparb in vitro. In vivo, it further induced transcription of Ppar target genes and caused changes in lipid composition of liver and plasma. The integrative approach provide a foundation for understanding how Ppars are engaged in regulating lipid metabolism in Atlantic cod physiology. We have shown that WY-14,643 and GW501516 activate Atlantic cod Ppara and Pparb, affect genes in lipid metabolism pathways, and induce changes in the lipid composition in plasma and liver microsomal membranes. Particularly, the combined transcriptomic, proteomics and lipidomics analyses revealed that effects of WY-14,643 on lipid metabolism are similar to what is known in mammalian studies, suggesting conservation of Ppara functions in mediating lipid metabolic processes in fish. The alterations in the lipid profiles observed after Ppar agonist exposure suggest that other chemicals with similar Ppar receptor affinities may cause disturbances in the lipid regulation of fish. Model organism: Atlantic cod (Gadus morhua). LSID: ... Article in Journal/Newspaper atlantic cod Gadus morhua North Atlantic Directory of Open Access Journals: DOAJ Articles Frontiers in Physiology 14 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
fish lipidomics transcriptomics proteomics WY-14,643 GW501516 Physiology QP1-981 |
spellingShingle |
fish lipidomics transcriptomics proteomics WY-14,643 GW501516 Physiology QP1-981 Marta Eide Anders Goksøyr Fekadu Yadetie Alejandra Gilabert Zdenka Bartosova Håvard G. Frøysa Shirin Fallahi Xiaokang Zhang Nello Blaser Inge Jonassen Per Bruheim Guttorm Alendal Morten Brun Cinta Porte Odd André Karlsen Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod |
topic_facet |
fish lipidomics transcriptomics proteomics WY-14,643 GW501516 Physiology QP1-981 |
description |
Lipid metabolism is essential in maintaining energy homeostasis in multicellular organisms. In vertebrates, the peroxisome proliferator-activated receptors (PPARs, NR1C) regulate the expression of many genes involved in these processes. Atlantic cod (Gadus morhua) is an important fish species in the North Atlantic ecosystem and in human nutrition, with a highly fatty liver. Here we study the involvement of Atlantic cod Ppar a and b subtypes in systemic regulation of lipid metabolism using two model agonists after in vivo exposure. WY-14,643, a specific PPARA ligand in mammals, activated cod Ppara1 and Ppara2 in vitro. In vivo, WY-14,643 caused a shift in lipid transport both at transcriptional and translational level in cod. However, WY-14,643 induced fewer genes in the fatty acid beta-oxidation pathway compared to that observed in rodents. Although GW501516 serves as a specific PPARB/D ligand in mammals, this compound activated cod Ppara1 and Ppara2 as well as Pparb in vitro. In vivo, it further induced transcription of Ppar target genes and caused changes in lipid composition of liver and plasma. The integrative approach provide a foundation for understanding how Ppars are engaged in regulating lipid metabolism in Atlantic cod physiology. We have shown that WY-14,643 and GW501516 activate Atlantic cod Ppara and Pparb, affect genes in lipid metabolism pathways, and induce changes in the lipid composition in plasma and liver microsomal membranes. Particularly, the combined transcriptomic, proteomics and lipidomics analyses revealed that effects of WY-14,643 on lipid metabolism are similar to what is known in mammalian studies, suggesting conservation of Ppara functions in mediating lipid metabolic processes in fish. The alterations in the lipid profiles observed after Ppar agonist exposure suggest that other chemicals with similar Ppar receptor affinities may cause disturbances in the lipid regulation of fish. Model organism: Atlantic cod (Gadus morhua). LSID: ... |
format |
Article in Journal/Newspaper |
author |
Marta Eide Anders Goksøyr Fekadu Yadetie Alejandra Gilabert Zdenka Bartosova Håvard G. Frøysa Shirin Fallahi Xiaokang Zhang Nello Blaser Inge Jonassen Per Bruheim Guttorm Alendal Morten Brun Cinta Porte Odd André Karlsen |
author_facet |
Marta Eide Anders Goksøyr Fekadu Yadetie Alejandra Gilabert Zdenka Bartosova Håvard G. Frøysa Shirin Fallahi Xiaokang Zhang Nello Blaser Inge Jonassen Per Bruheim Guttorm Alendal Morten Brun Cinta Porte Odd André Karlsen |
author_sort |
Marta Eide |
title |
Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod |
title_short |
Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod |
title_full |
Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod |
title_fullStr |
Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod |
title_full_unstemmed |
Integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male Atlantic cod |
title_sort |
integrative omics-analysis of lipid metabolism regulation by peroxisome proliferator-activated receptor a and b agonists in male atlantic cod |
publisher |
Frontiers Media S.A. |
publishDate |
2023 |
url |
https://doi.org/10.3389/fphys.2023.1129089 https://doaj.org/article/b68b5da6a8394e70bf75e8248ce668ce |
genre |
atlantic cod Gadus morhua North Atlantic |
genre_facet |
atlantic cod Gadus morhua North Atlantic |
op_source |
Frontiers in Physiology, Vol 14 (2023) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fphys.2023.1129089/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2023.1129089 https://doaj.org/article/b68b5da6a8394e70bf75e8248ce668ce |
op_doi |
https://doi.org/10.3389/fphys.2023.1129089 |
container_title |
Frontiers in Physiology |
container_volume |
14 |
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1766357217763655680 |