Characterization of Cnidoscolus quercifolius Pohl bark root extract and evaluation of cytotoxic effect on human tumor cell lines
Objective: To evaluate the chemical components of active extract from Cnidoscolus quercifolius root bark and its cytotoxic potential against several tumor strains. Methods: The high-performance liquid chromatography with diode-array detection and 1H and 13C nuclear magnetic resonance spectroscopy of...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2018
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Online Access: | https://doi.org/10.4103/2221-1691.237077 https://doaj.org/article/b66b989d938a46108a73ac2f4cd1a335 |
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ftdoajarticles:oai:doaj.org/article:b66b989d938a46108a73ac2f4cd1a335 2023-05-15T15:10:32+02:00 Characterization of Cnidoscolus quercifolius Pohl bark root extract and evaluation of cytotoxic effect on human tumor cell lines Paulo Fernando Machado Paredes Selene Maia de Morais Fernando César Rodrigues Brito Luiz Francisco Wemmenson Gonçalves Moura Patrícia de Araújo Rodrigues Stephen Rathinaraj Benjamin Francisco Ernani Alves Magalhães Eridan Orlando Pereira Tramontina Florean Maria Izabel Florindo Guedes 2018-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.237077 https://doaj.org/article/b66b989d938a46108a73ac2f4cd1a335 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=7;spage=345;epage=351;aulast=Paredes https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.237077 https://doaj.org/article/b66b989d938a46108a73ac2f4cd1a335 Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 7, Pp 345-351 (2018) cnidoscolus quercifolius pohl. methylfaveline deoxofaveline neofavelanone faveline cytotoxicity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.4103/2221-1691.237077 2022-12-30T22:57:15Z Objective: To evaluate the chemical components of active extract from Cnidoscolus quercifolius root bark and its cytotoxic potential against several tumor strains. Methods: The high-performance liquid chromatography with diode-array detection and 1H and 13C nuclear magnetic resonance spectroscopy of the extract were used to distinguish the existence of possible functional groups in the root bark extract. The in vitro cytotoxic activity of methanol extract on human colon cancer cell lines was evaluated using OVCAR-8, SF-295, HCT-116, HL-60 strains and the samples were assessed by 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide method. Results: The analysis of nuclear magnetic spectra of the active chloroform fraction revealed the presence of absorptions bands correspondent to a mixture of favelines such as neofavelanone, deoxofaveline or methyl-faveline, which structures were confirmed by ultraviolet spectra upon high-performance liquid chromatography with diode-array detection analysis. The active fraction showed cytotoxic effects in the tested strains, HCT-116, SF-295, OVCAR-8 and HL-60 cells with IC50 of 72 hours ranging from 4.95 to 15.23 μg/mL. Conclusions: The results suggest that the substances present in faveleira (Cnidoscolus quercifolius) root bark extract have a cytotoxic potential against several tumor lines, showing a broader antitumour potential, and in addition no adverse to healthy cells. Therefore, the root bark extract of Cnidoscolus quercifolius has a possibility of use for anticarcinogenic therapies. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 8 7 345 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
cnidoscolus quercifolius pohl. methylfaveline deoxofaveline neofavelanone faveline cytotoxicity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
cnidoscolus quercifolius pohl. methylfaveline deoxofaveline neofavelanone faveline cytotoxicity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Paulo Fernando Machado Paredes Selene Maia de Morais Fernando César Rodrigues Brito Luiz Francisco Wemmenson Gonçalves Moura Patrícia de Araújo Rodrigues Stephen Rathinaraj Benjamin Francisco Ernani Alves Magalhães Eridan Orlando Pereira Tramontina Florean Maria Izabel Florindo Guedes Characterization of Cnidoscolus quercifolius Pohl bark root extract and evaluation of cytotoxic effect on human tumor cell lines |
topic_facet |
cnidoscolus quercifolius pohl. methylfaveline deoxofaveline neofavelanone faveline cytotoxicity Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the chemical components of active extract from Cnidoscolus quercifolius root bark and its cytotoxic potential against several tumor strains. Methods: The high-performance liquid chromatography with diode-array detection and 1H and 13C nuclear magnetic resonance spectroscopy of the extract were used to distinguish the existence of possible functional groups in the root bark extract. The in vitro cytotoxic activity of methanol extract on human colon cancer cell lines was evaluated using OVCAR-8, SF-295, HCT-116, HL-60 strains and the samples were assessed by 3-(4,5-dimethylthiazol2-yl)-2,5-diphenyltetrazolium bromide method. Results: The analysis of nuclear magnetic spectra of the active chloroform fraction revealed the presence of absorptions bands correspondent to a mixture of favelines such as neofavelanone, deoxofaveline or methyl-faveline, which structures were confirmed by ultraviolet spectra upon high-performance liquid chromatography with diode-array detection analysis. The active fraction showed cytotoxic effects in the tested strains, HCT-116, SF-295, OVCAR-8 and HL-60 cells with IC50 of 72 hours ranging from 4.95 to 15.23 μg/mL. Conclusions: The results suggest that the substances present in faveleira (Cnidoscolus quercifolius) root bark extract have a cytotoxic potential against several tumor lines, showing a broader antitumour potential, and in addition no adverse to healthy cells. Therefore, the root bark extract of Cnidoscolus quercifolius has a possibility of use for anticarcinogenic therapies. |
format |
Article in Journal/Newspaper |
author |
Paulo Fernando Machado Paredes Selene Maia de Morais Fernando César Rodrigues Brito Luiz Francisco Wemmenson Gonçalves Moura Patrícia de Araújo Rodrigues Stephen Rathinaraj Benjamin Francisco Ernani Alves Magalhães Eridan Orlando Pereira Tramontina Florean Maria Izabel Florindo Guedes |
author_facet |
Paulo Fernando Machado Paredes Selene Maia de Morais Fernando César Rodrigues Brito Luiz Francisco Wemmenson Gonçalves Moura Patrícia de Araújo Rodrigues Stephen Rathinaraj Benjamin Francisco Ernani Alves Magalhães Eridan Orlando Pereira Tramontina Florean Maria Izabel Florindo Guedes |
author_sort |
Paulo Fernando Machado Paredes |
title |
Characterization of Cnidoscolus quercifolius Pohl bark root extract and evaluation of cytotoxic effect on human tumor cell lines |
title_short |
Characterization of Cnidoscolus quercifolius Pohl bark root extract and evaluation of cytotoxic effect on human tumor cell lines |
title_full |
Characterization of Cnidoscolus quercifolius Pohl bark root extract and evaluation of cytotoxic effect on human tumor cell lines |
title_fullStr |
Characterization of Cnidoscolus quercifolius Pohl bark root extract and evaluation of cytotoxic effect on human tumor cell lines |
title_full_unstemmed |
Characterization of Cnidoscolus quercifolius Pohl bark root extract and evaluation of cytotoxic effect on human tumor cell lines |
title_sort |
characterization of cnidoscolus quercifolius pohl bark root extract and evaluation of cytotoxic effect on human tumor cell lines |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/2221-1691.237077 https://doaj.org/article/b66b989d938a46108a73ac2f4cd1a335 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 7, Pp 345-351 (2018) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=7;spage=345;epage=351;aulast=Paredes https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.237077 https://doaj.org/article/b66b989d938a46108a73ac2f4cd1a335 |
op_doi |
https://doi.org/10.4103/2221-1691.237077 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
8 |
container_issue |
7 |
container_start_page |
345 |
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1766341544350056448 |