The Mitogenome of the Subarctic Octocoral Alcyonium digitatum Reveals a Putative tRNA Pro Gene Nested within MutS

• Published in: Current Issues in Molecular Biology
• Main Authors: Alisa Heuchel, Åse Emblem, Tor Erik Jørgensen, Truls Moum, Steinar Daae Johansen
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2024
• Subjects: Alcyoniidae; dual coding; mitochondrial genome; octocoral; phylogeny; tRNA; Biology (General); QH301-705.5; Subarctic
• Online Access: https://doi.org/10.3390/cimb46080479; https://doaj.org/article/b6560eea6390491190b19ca281503f37

We sequenced and analyzed the complete mitogenome of a Norwegian isolate of the octocoral Alcyonium digitatum using the Ion Torrent sequencing technology. The 18,790 bp circular mitochondrial genome was found to harbor the same set of 17 genes, which encode 14 protein subunits, two structural ribosomal RNAs and one tRNA, as reported in other octocorals. In addition, we detected a new tRNA Pro -like gene sequence nested within the MutS protein coding region. This putative tRNA gene feature appears to be conserved among the octocorals but has not been reported previously. The A. digitatum mitogenome was also shown to harbor an optional gene (ORFA) that encodes a putative protein of 191 amino acids with unknown function. A mitogenome-based phylogenetic analysis, presented as a maximum likelihood tree, showed that A. digitatum clustered with high statistical confidence with two other Alcyonium species endemic to the Mediterranean Sea and the Southeast Pacific Ocean.