A study of the HIV-1 regulatory genes using the polymerase chain reaction
In this work, a total of 200 samples from the HIV-infected individuals were analyzed: 50 samples from the Saha Republic (Yakutia), 50 samples from the Vologda Region (City of Cherepovets), and 100 samples from the Moscow Region (Moscow and Moscow Region). All samples were obtained from the patients...
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Central Research Institute for Epidemiology
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ftdoajarticles:oai:doaj.org/article:b6335de194934736b9c23fc1a344caba 2023-07-30T04:07:27+02:00 A study of the HIV-1 regulatory genes using the polymerase chain reaction K. A. Ryzhov M. N. Nosik A. V. Kravtchenko 2015-06-01T00:00:00Z https://doaj.org/article/b6335de194934736b9c23fc1a344caba EN RU eng rus Central Research Institute for Epidemiology https://virusjour.crie.ru/jour/article/viewFile/333/211 https://doaj.org/toc/0507-4088 https://doaj.org/toc/2411-2097 0507-4088 2411-2097 https://doaj.org/article/b6335de194934736b9c23fc1a344caba Вопросы вирусологии, Vol 60, Iss 3, Pp 41-44 (2015) hiv regulatory genes pcr nested pcr stages of hiv-infection Microbiology QR1-502 article 2015 ftdoajarticles 2023-07-16T00:36:07Z In this work, a total of 200 samples from the HIV-infected individuals were analyzed: 50 samples from the Saha Republic (Yakutia), 50 samples from the Vologda Region (City of Cherepovets), and 100 samples from the Moscow Region (Moscow and Moscow Region). All samples were obtained from the patients who were not undergoing antiretroviral therapy. It was detected that the regulatory genes vif, vpr, vpu, rev, tat, and nef were amplified with moderate sensitivity after one-stage amplification. When those samples were analyzed by the nested PCR the detection ratio was much higher. While studying nef-gene the phenomena of the splicing in cells cores was detected at the advanced stages of the HIV-infection (3 and 4 stages). At the same time, the splicing was not detected at the earlier stages of the HIV-infection. This effect might be the cause of the transition from asymptomatic stage of the infection to the advanced stage. It was also shown for the first time that the variability of the regulatory genes correlated with the virus subtype. Article in Journal/Newspaper Yakutia Directory of Open Access Journals: DOAJ Articles |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English Russian |
topic |
hiv regulatory genes pcr nested pcr stages of hiv-infection Microbiology QR1-502 |
spellingShingle |
hiv regulatory genes pcr nested pcr stages of hiv-infection Microbiology QR1-502 K. A. Ryzhov M. N. Nosik A. V. Kravtchenko A study of the HIV-1 regulatory genes using the polymerase chain reaction |
topic_facet |
hiv regulatory genes pcr nested pcr stages of hiv-infection Microbiology QR1-502 |
description |
In this work, a total of 200 samples from the HIV-infected individuals were analyzed: 50 samples from the Saha Republic (Yakutia), 50 samples from the Vologda Region (City of Cherepovets), and 100 samples from the Moscow Region (Moscow and Moscow Region). All samples were obtained from the patients who were not undergoing antiretroviral therapy. It was detected that the regulatory genes vif, vpr, vpu, rev, tat, and nef were amplified with moderate sensitivity after one-stage amplification. When those samples were analyzed by the nested PCR the detection ratio was much higher. While studying nef-gene the phenomena of the splicing in cells cores was detected at the advanced stages of the HIV-infection (3 and 4 stages). At the same time, the splicing was not detected at the earlier stages of the HIV-infection. This effect might be the cause of the transition from asymptomatic stage of the infection to the advanced stage. It was also shown for the first time that the variability of the regulatory genes correlated with the virus subtype. |
format |
Article in Journal/Newspaper |
author |
K. A. Ryzhov M. N. Nosik A. V. Kravtchenko |
author_facet |
K. A. Ryzhov M. N. Nosik A. V. Kravtchenko |
author_sort |
K. A. Ryzhov |
title |
A study of the HIV-1 regulatory genes using the polymerase chain reaction |
title_short |
A study of the HIV-1 regulatory genes using the polymerase chain reaction |
title_full |
A study of the HIV-1 regulatory genes using the polymerase chain reaction |
title_fullStr |
A study of the HIV-1 regulatory genes using the polymerase chain reaction |
title_full_unstemmed |
A study of the HIV-1 regulatory genes using the polymerase chain reaction |
title_sort |
study of the hiv-1 regulatory genes using the polymerase chain reaction |
publisher |
Central Research Institute for Epidemiology |
publishDate |
2015 |
url |
https://doaj.org/article/b6335de194934736b9c23fc1a344caba |
genre |
Yakutia |
genre_facet |
Yakutia |
op_source |
Вопросы вирусологии, Vol 60, Iss 3, Pp 41-44 (2015) |
op_relation |
https://virusjour.crie.ru/jour/article/viewFile/333/211 https://doaj.org/toc/0507-4088 https://doaj.org/toc/2411-2097 0507-4088 2411-2097 https://doaj.org/article/b6335de194934736b9c23fc1a344caba |
_version_ |
1772820749221888000 |