Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae
Abstract Objective The eukaryotic plasma membrane localized light-gated proton-pumping rhodopsins possesses great optogenetic applications for repolarization (silencing) of the neuronal activity simply by light illumination. Very few plasma membrane localized proton-pumping rhodopsins of a eukaryoti...
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ftdoajarticles:oai:doaj.org/article:b61e8f8c619c4923b11513c24dd7f406 2023-05-15T13:46:53+02:00 Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae Peeyush Ranjan Suneel Kateriya 2018-01-01T00:00:00Z https://doi.org/10.1186/s13104-018-3181-4 https://doaj.org/article/b61e8f8c619c4923b11513c24dd7f406 EN eng BMC http://link.springer.com/article/10.1186/s13104-018-3181-4 https://doaj.org/toc/1756-0500 doi:10.1186/s13104-018-3181-4 1756-0500 https://doaj.org/article/b61e8f8c619c4923b11513c24dd7f406 BMC Research Notes, Vol 11, Iss 1, Pp 1-7 (2018) Coccomyxa subellipsoidea Proton-pumping rhodopsin Optogenetics Localization of the microbial rhodopsin in eukaryotes Chlorella vulgaris Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 article 2018 ftdoajarticles https://doi.org/10.1186/s13104-018-3181-4 2022-12-31T11:22:26Z Abstract Objective The eukaryotic plasma membrane localized light-gated proton-pumping rhodopsins possesses great optogenetic applications for repolarization (silencing) of the neuronal activity simply by light illumination. Very few plasma membrane localized proton-pumping rhodopsins of a eukaryotic origin are known that have optogenetic potential. Our objective was to identify and characterize microbial rhodopsin of an eukaryotic origin that expresses on plasma membrane. The plasma membrane localized light-gated proton pump of an eukaryotic origin hold great promise to be used as an optogenetic tools for the neurobiology. Results Here, we had characterized the cellular expression and membrane localization of a new rhodopsin in Antarctican algae Coccomyxa subellipsoidea. It is the first algal ion pumping rhodopsin that localizes to the plasma membrane of the eukaryotic cells. Coccomyxa subellipsoidea rhodopsin exists in the monomeric and dimeric state both the in vivo and in vitro. The dimeric form of the Coccomyxa subellipsoidea rhodopsin is resistant to heat and detergent denaturants. Article in Journal/Newspaper Antarc* Directory of Open Access Journals: DOAJ Articles BMC Research Notes 11 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Coccomyxa subellipsoidea Proton-pumping rhodopsin Optogenetics Localization of the microbial rhodopsin in eukaryotes Chlorella vulgaris Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 |
spellingShingle |
Coccomyxa subellipsoidea Proton-pumping rhodopsin Optogenetics Localization of the microbial rhodopsin in eukaryotes Chlorella vulgaris Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 Peeyush Ranjan Suneel Kateriya Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
topic_facet |
Coccomyxa subellipsoidea Proton-pumping rhodopsin Optogenetics Localization of the microbial rhodopsin in eukaryotes Chlorella vulgaris Medicine R Biology (General) QH301-705.5 Science (General) Q1-390 |
description |
Abstract Objective The eukaryotic plasma membrane localized light-gated proton-pumping rhodopsins possesses great optogenetic applications for repolarization (silencing) of the neuronal activity simply by light illumination. Very few plasma membrane localized proton-pumping rhodopsins of a eukaryotic origin are known that have optogenetic potential. Our objective was to identify and characterize microbial rhodopsin of an eukaryotic origin that expresses on plasma membrane. The plasma membrane localized light-gated proton pump of an eukaryotic origin hold great promise to be used as an optogenetic tools for the neurobiology. Results Here, we had characterized the cellular expression and membrane localization of a new rhodopsin in Antarctican algae Coccomyxa subellipsoidea. It is the first algal ion pumping rhodopsin that localizes to the plasma membrane of the eukaryotic cells. Coccomyxa subellipsoidea rhodopsin exists in the monomeric and dimeric state both the in vivo and in vitro. The dimeric form of the Coccomyxa subellipsoidea rhodopsin is resistant to heat and detergent denaturants. |
format |
Article in Journal/Newspaper |
author |
Peeyush Ranjan Suneel Kateriya |
author_facet |
Peeyush Ranjan Suneel Kateriya |
author_sort |
Peeyush Ranjan |
title |
Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
title_short |
Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
title_full |
Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
title_fullStr |
Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
title_full_unstemmed |
Localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
title_sort |
localization and dimer stability of a newly identified microbial rhodopsin from a polar, non-motile green algae |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s13104-018-3181-4 https://doaj.org/article/b61e8f8c619c4923b11513c24dd7f406 |
genre |
Antarc* |
genre_facet |
Antarc* |
op_source |
BMC Research Notes, Vol 11, Iss 1, Pp 1-7 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s13104-018-3181-4 https://doaj.org/toc/1756-0500 doi:10.1186/s13104-018-3181-4 1756-0500 https://doaj.org/article/b61e8f8c619c4923b11513c24dd7f406 |
op_doi |
https://doi.org/10.1186/s13104-018-3181-4 |
container_title |
BMC Research Notes |
container_volume |
11 |
container_issue |
1 |
_version_ |
1766245509557649408 |