Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in Northwestern Turkey
INTRODUCTION: Antibiotic resistance is the main factor that affects the efficacy of current therapeutic regimens against Helicobacter pylori. This study aimed to determine the rates of resistance to efficacy clarithromycin, amoxicillin, tetracycline, levofloxacin and metronidazole among H. pylori st...
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ftdoajarticles:oai:doaj.org/article:b601e325ef474fc693de4aada2fde052 2023-05-15T15:10:37+02:00 Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in Northwestern Turkey Reyhan Caliskan Hrisi Bahar Tokman Yusuf Erzin Suat Saribas Pelin Yuksel Bora Kazim Bolek Ecehan Ozge Sevuk Mehmet Demirci Ozge Yılmazli Ozer Akgul Fatma Kalayci Huseyin Cakan Barik Salih Kadir Bal Bekir Kocazeybek 2015-06-01T00:00:00Z https://doi.org/10.1590/0037-8682-0027-2015 https://doaj.org/article/b601e325ef474fc693de4aada2fde052 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000300278&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0027-2015 https://doaj.org/article/b601e325ef474fc693de4aada2fde052 Revista da Sociedade Brasileira de Medicina Tropical, Vol 48, Iss 3, Pp 278-284 (2015) Helicobacter pylori Levofloxacin Clarithromycin Amoxicillin Tetracycline Metronidazole Arctic medicine. Tropical medicine RC955-962 article 2015 ftdoajarticles https://doi.org/10.1590/0037-8682-0027-2015 2022-12-31T02:52:31Z INTRODUCTION: Antibiotic resistance is the main factor that affects the efficacy of current therapeutic regimens against Helicobacter pylori. This study aimed to determine the rates of resistance to efficacy clarithromycin, amoxicillin, tetracycline, levofloxacin and metronidazole among H. pylori strains isolated from Turkish patients with dyspepsia. METHODS: H. pylori was cultured from corpus and antrum biopsies that were collected from patients with dyspeptic symptoms, and the antimicrobial susceptibility of H. pylori was determined using the E-test (clarithromycin, amoxicillin, tetracycline, metronidazole and levofloxacin) according to the EUCAST breakpoints. Point mutations in the 23S rRNA gene of clarithromycin-resistant strains were investigated using real-time PCR. RESULTS: A total of 98 H. pylori strains were isolated, all of which were susceptible to amoxicillin and tetracycline. Of these strains, 36.7% (36/98) were resistant to clarithromycin, 35.5% (34/98) were resistant to metronidazole, and 29.5% (29/98) were resistant to levofloxacin. Multiple resistance was detected in 19.3% of the isolates. The A2143G and A2144G point mutations in the 23S rRNA-encoding gene were found in all 36 (100%) of the clarithromycin-resistant strains. Additionally, the levofloxacin MIC values increased to 32 mg/L in our H. pylori strains. Finally, among the clarithromycin-resistant strains, 27.2% were resistant to levofloxacin, and 45.4% were resistant to metronidazole. CONCLUSIONS: We conclude that treatment failure after clarithromycin- or levofloxacin-based triple therapy is not surprising and that metronidazole is not a reliable agent for the eradication of H. pylori infection in Turkey. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 48 3 278 284 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Helicobacter pylori Levofloxacin Clarithromycin Amoxicillin Tetracycline Metronidazole Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Helicobacter pylori Levofloxacin Clarithromycin Amoxicillin Tetracycline Metronidazole Arctic medicine. Tropical medicine RC955-962 Reyhan Caliskan Hrisi Bahar Tokman Yusuf Erzin Suat Saribas Pelin Yuksel Bora Kazim Bolek Ecehan Ozge Sevuk Mehmet Demirci Ozge Yılmazli Ozer Akgul Fatma Kalayci Huseyin Cakan Barik Salih Kadir Bal Bekir Kocazeybek Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in Northwestern Turkey |
topic_facet |
Helicobacter pylori Levofloxacin Clarithromycin Amoxicillin Tetracycline Metronidazole Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Antibiotic resistance is the main factor that affects the efficacy of current therapeutic regimens against Helicobacter pylori. This study aimed to determine the rates of resistance to efficacy clarithromycin, amoxicillin, tetracycline, levofloxacin and metronidazole among H. pylori strains isolated from Turkish patients with dyspepsia. METHODS: H. pylori was cultured from corpus and antrum biopsies that were collected from patients with dyspeptic symptoms, and the antimicrobial susceptibility of H. pylori was determined using the E-test (clarithromycin, amoxicillin, tetracycline, metronidazole and levofloxacin) according to the EUCAST breakpoints. Point mutations in the 23S rRNA gene of clarithromycin-resistant strains were investigated using real-time PCR. RESULTS: A total of 98 H. pylori strains were isolated, all of which were susceptible to amoxicillin and tetracycline. Of these strains, 36.7% (36/98) were resistant to clarithromycin, 35.5% (34/98) were resistant to metronidazole, and 29.5% (29/98) were resistant to levofloxacin. Multiple resistance was detected in 19.3% of the isolates. The A2143G and A2144G point mutations in the 23S rRNA-encoding gene were found in all 36 (100%) of the clarithromycin-resistant strains. Additionally, the levofloxacin MIC values increased to 32 mg/L in our H. pylori strains. Finally, among the clarithromycin-resistant strains, 27.2% were resistant to levofloxacin, and 45.4% were resistant to metronidazole. CONCLUSIONS: We conclude that treatment failure after clarithromycin- or levofloxacin-based triple therapy is not surprising and that metronidazole is not a reliable agent for the eradication of H. pylori infection in Turkey. |
format |
Article in Journal/Newspaper |
author |
Reyhan Caliskan Hrisi Bahar Tokman Yusuf Erzin Suat Saribas Pelin Yuksel Bora Kazim Bolek Ecehan Ozge Sevuk Mehmet Demirci Ozge Yılmazli Ozer Akgul Fatma Kalayci Huseyin Cakan Barik Salih Kadir Bal Bekir Kocazeybek |
author_facet |
Reyhan Caliskan Hrisi Bahar Tokman Yusuf Erzin Suat Saribas Pelin Yuksel Bora Kazim Bolek Ecehan Ozge Sevuk Mehmet Demirci Ozge Yılmazli Ozer Akgul Fatma Kalayci Huseyin Cakan Barik Salih Kadir Bal Bekir Kocazeybek |
author_sort |
Reyhan Caliskan |
title |
Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in Northwestern Turkey |
title_short |
Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in Northwestern Turkey |
title_full |
Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in Northwestern Turkey |
title_fullStr |
Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in Northwestern Turkey |
title_full_unstemmed |
Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in Northwestern Turkey |
title_sort |
antimicrobial resistance of helicobacter pylori strains to five antibiotics, including levofloxacin, in northwestern turkey |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2015 |
url |
https://doi.org/10.1590/0037-8682-0027-2015 https://doaj.org/article/b601e325ef474fc693de4aada2fde052 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 48, Iss 3, Pp 278-284 (2015) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822015000300278&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0027-2015 https://doaj.org/article/b601e325ef474fc693de4aada2fde052 |
op_doi |
https://doi.org/10.1590/0037-8682-0027-2015 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
48 |
container_issue |
3 |
container_start_page |
278 |
op_container_end_page |
284 |
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1766341610890592256 |