Identification of a toxin coding fragment in pBSSB1, a linear plasmid from Salmonella enterica serovar Typhi that can stabilize a multicopy plasmid
Objective: To identify the region conferring stability to pBSSB2 (a linear plasmid, pBSSB1, containing a kanamycin cassette), which is unique to Indonesian isolates of Salmonella enterica serovar Typhi. Methods: The open reading frame (ORF) 009 was identified as a toxin coding gene in the plasmid th...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:b5e1b38b5c7541dd84420864bf32c9f1 2023-05-15T15:06:10+02:00 Identification of a toxin coding fragment in pBSSB1, a linear plasmid from Salmonella enterica serovar Typhi that can stabilize a multicopy plasmid Sunjukta Ahsan David Summers 2018-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.237080 https://doaj.org/article/b5e1b38b5c7541dd84420864bf32c9f1 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=7;spage=365;epage=370;aulast=Ahsan https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.237080 https://doaj.org/article/b5e1b38b5c7541dd84420864bf32c9f1 Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 7, Pp 365-370 (2018) linear plasmid pbssb2 salmonella enterica serovar typhi Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.4103/2221-1691.237080 2022-12-31T01:23:21Z Objective: To identify the region conferring stability to pBSSB2 (a linear plasmid, pBSSB1, containing a kanamycin cassette), which is unique to Indonesian isolates of Salmonella enterica serovar Typhi. Methods: The open reading frame (ORF) 009 was identified as a toxin coding gene in the plasmid through introduction of translational termination codons in the ORF. Results: The stability function was located in a fragment that spanned nucleotides 5 766 to 6 828 in the linear plasmid genetic map. Ectopic expression of ORF009 in pBAD18 vector indicated ORF009 codes for a toxin. This fragment could stabilize plasmid pUC18 previously destabilized through mutation of the pcnB (plasmid copy number control) gene that codes for polyA polymerase. Majority of the cells expressing ORF009 were non-viable according to phase contrast microscopy. Conclusions: This study demonstrated that a linear plasmid fragment that carries a gene encoding a toxin possibly conferred stability to the parent plasmid. It was able to stabilize a multicopy plasmid of Escherichia coli. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 8 7 365 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
linear plasmid pbssb2 salmonella enterica serovar typhi Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
linear plasmid pbssb2 salmonella enterica serovar typhi Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Sunjukta Ahsan David Summers Identification of a toxin coding fragment in pBSSB1, a linear plasmid from Salmonella enterica serovar Typhi that can stabilize a multicopy plasmid |
topic_facet |
linear plasmid pbssb2 salmonella enterica serovar typhi Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To identify the region conferring stability to pBSSB2 (a linear plasmid, pBSSB1, containing a kanamycin cassette), which is unique to Indonesian isolates of Salmonella enterica serovar Typhi. Methods: The open reading frame (ORF) 009 was identified as a toxin coding gene in the plasmid through introduction of translational termination codons in the ORF. Results: The stability function was located in a fragment that spanned nucleotides 5 766 to 6 828 in the linear plasmid genetic map. Ectopic expression of ORF009 in pBAD18 vector indicated ORF009 codes for a toxin. This fragment could stabilize plasmid pUC18 previously destabilized through mutation of the pcnB (plasmid copy number control) gene that codes for polyA polymerase. Majority of the cells expressing ORF009 were non-viable according to phase contrast microscopy. Conclusions: This study demonstrated that a linear plasmid fragment that carries a gene encoding a toxin possibly conferred stability to the parent plasmid. It was able to stabilize a multicopy plasmid of Escherichia coli. |
format |
Article in Journal/Newspaper |
author |
Sunjukta Ahsan David Summers |
author_facet |
Sunjukta Ahsan David Summers |
author_sort |
Sunjukta Ahsan |
title |
Identification of a toxin coding fragment in pBSSB1, a linear plasmid from Salmonella enterica serovar Typhi that can stabilize a multicopy plasmid |
title_short |
Identification of a toxin coding fragment in pBSSB1, a linear plasmid from Salmonella enterica serovar Typhi that can stabilize a multicopy plasmid |
title_full |
Identification of a toxin coding fragment in pBSSB1, a linear plasmid from Salmonella enterica serovar Typhi that can stabilize a multicopy plasmid |
title_fullStr |
Identification of a toxin coding fragment in pBSSB1, a linear plasmid from Salmonella enterica serovar Typhi that can stabilize a multicopy plasmid |
title_full_unstemmed |
Identification of a toxin coding fragment in pBSSB1, a linear plasmid from Salmonella enterica serovar Typhi that can stabilize a multicopy plasmid |
title_sort |
identification of a toxin coding fragment in pbssb1, a linear plasmid from salmonella enterica serovar typhi that can stabilize a multicopy plasmid |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/2221-1691.237080 https://doaj.org/article/b5e1b38b5c7541dd84420864bf32c9f1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 7, Pp 365-370 (2018) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=7;spage=365;epage=370;aulast=Ahsan https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.237080 https://doaj.org/article/b5e1b38b5c7541dd84420864bf32c9f1 |
op_doi |
https://doi.org/10.4103/2221-1691.237080 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
8 |
container_issue |
7 |
container_start_page |
365 |
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1766337825138016256 |