Wide Distribution and Genetic Diversity of Babesia microti in Small Mammals from Yunnan Province, Southwestern China.
BACKGROUND:Babesia, usually found in wild and domestic mammals worldwide, have recently been responsible for emerging malaria-like zoonosis in infected patients. Human B. microti infection has been identified in China, primarily in the Southwest along the Myanmar border but little direct surveillanc...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0005898 https://doaj.org/article/b5b77bd4ef714c47afcb6d6f9f8d6f7c |
id |
ftdoajarticles:oai:doaj.org/article:b5b77bd4ef714c47afcb6d6f9f8d6f7c |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:b5b77bd4ef714c47afcb6d6f9f8d6f7c 2023-05-15T15:11:20+02:00 Wide Distribution and Genetic Diversity of Babesia microti in Small Mammals from Yunnan Province, Southwestern China. Zi-Hou Gao Tao-Hua Huang Bao-Gui Jiang Na Jia Zheng-Xiang Liu Zong-Ti Shao Rui-Ruo Jiang Hong-Bo Liu Ran Wei Yu-Qiong Li Hong-Wu Yao Michael E von Fricken Jia-Fu Jiang Chun-Hong Du Wu-Chun Cao 2017-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005898 https://doaj.org/article/b5b77bd4ef714c47afcb6d6f9f8d6f7c EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0005898 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005898 https://doaj.org/article/b5b77bd4ef714c47afcb6d6f9f8d6f7c PLoS Neglected Tropical Diseases, Vol 11, Iss 10, p e0005898 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005898 2022-12-31T06:00:24Z BACKGROUND:Babesia, usually found in wild and domestic mammals worldwide, have recently been responsible for emerging malaria-like zoonosis in infected patients. Human B. microti infection has been identified in China, primarily in the Southwest along the Myanmar border but little direct surveillance of B. microti infection in rodents has been carried out here (Yunnan province). In this region, a diverse topographic range combined with tropical moisture sustains a high biodiversity of small mammals, which might play important role on Babesia transmission. METHODS:Small mammals were captured in 141 sample locations from 18 counties located Yunnan Province, and screened for B. microti-like parasites infection by a nested PCR to target 18S rRNA gene of Babesia, plus directly sequencing for positive samples. Univariate and multivariate forward stepwise logistic regression analysis was used to access the association between infections and some related risk factors. RESULTS:Infection with Babesia microti was confirmed in 2.4% (53/ 2204) of small mammals. Significant differences in prevalence rates of B. microti were observed based on variations in forest, agricultural, and residential landscapes. Furthermore, adult small mammals had higher prevalence rates than younger, pubertal mammals. The near full-length 18S rRNA gene revealed that there were two types of B. microti, Kobe and Otsu, which demonstrate the genetic diversity and regional distribution. CONCLUSIONS:There exists a wide distribution and genetic diversity of endemic B. microti in Southwestern China, warranting further investigations and monitoring of clinical disease in individuals presenting with Babesia like symptoms in these areas. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 10 e0005898 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Zi-Hou Gao Tao-Hua Huang Bao-Gui Jiang Na Jia Zheng-Xiang Liu Zong-Ti Shao Rui-Ruo Jiang Hong-Bo Liu Ran Wei Yu-Qiong Li Hong-Wu Yao Michael E von Fricken Jia-Fu Jiang Chun-Hong Du Wu-Chun Cao Wide Distribution and Genetic Diversity of Babesia microti in Small Mammals from Yunnan Province, Southwestern China. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Babesia, usually found in wild and domestic mammals worldwide, have recently been responsible for emerging malaria-like zoonosis in infected patients. Human B. microti infection has been identified in China, primarily in the Southwest along the Myanmar border but little direct surveillance of B. microti infection in rodents has been carried out here (Yunnan province). In this region, a diverse topographic range combined with tropical moisture sustains a high biodiversity of small mammals, which might play important role on Babesia transmission. METHODS:Small mammals were captured in 141 sample locations from 18 counties located Yunnan Province, and screened for B. microti-like parasites infection by a nested PCR to target 18S rRNA gene of Babesia, plus directly sequencing for positive samples. Univariate and multivariate forward stepwise logistic regression analysis was used to access the association between infections and some related risk factors. RESULTS:Infection with Babesia microti was confirmed in 2.4% (53/ 2204) of small mammals. Significant differences in prevalence rates of B. microti were observed based on variations in forest, agricultural, and residential landscapes. Furthermore, adult small mammals had higher prevalence rates than younger, pubertal mammals. The near full-length 18S rRNA gene revealed that there were two types of B. microti, Kobe and Otsu, which demonstrate the genetic diversity and regional distribution. CONCLUSIONS:There exists a wide distribution and genetic diversity of endemic B. microti in Southwestern China, warranting further investigations and monitoring of clinical disease in individuals presenting with Babesia like symptoms in these areas. |
format |
Article in Journal/Newspaper |
author |
Zi-Hou Gao Tao-Hua Huang Bao-Gui Jiang Na Jia Zheng-Xiang Liu Zong-Ti Shao Rui-Ruo Jiang Hong-Bo Liu Ran Wei Yu-Qiong Li Hong-Wu Yao Michael E von Fricken Jia-Fu Jiang Chun-Hong Du Wu-Chun Cao |
author_facet |
Zi-Hou Gao Tao-Hua Huang Bao-Gui Jiang Na Jia Zheng-Xiang Liu Zong-Ti Shao Rui-Ruo Jiang Hong-Bo Liu Ran Wei Yu-Qiong Li Hong-Wu Yao Michael E von Fricken Jia-Fu Jiang Chun-Hong Du Wu-Chun Cao |
author_sort |
Zi-Hou Gao |
title |
Wide Distribution and Genetic Diversity of Babesia microti in Small Mammals from Yunnan Province, Southwestern China. |
title_short |
Wide Distribution and Genetic Diversity of Babesia microti in Small Mammals from Yunnan Province, Southwestern China. |
title_full |
Wide Distribution and Genetic Diversity of Babesia microti in Small Mammals from Yunnan Province, Southwestern China. |
title_fullStr |
Wide Distribution and Genetic Diversity of Babesia microti in Small Mammals from Yunnan Province, Southwestern China. |
title_full_unstemmed |
Wide Distribution and Genetic Diversity of Babesia microti in Small Mammals from Yunnan Province, Southwestern China. |
title_sort |
wide distribution and genetic diversity of babesia microti in small mammals from yunnan province, southwestern china. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005898 https://doaj.org/article/b5b77bd4ef714c47afcb6d6f9f8d6f7c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 10, p e0005898 (2017) |
op_relation |
https://doi.org/10.1371/journal.pntd.0005898 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005898 https://doaj.org/article/b5b77bd4ef714c47afcb6d6f9f8d6f7c |
op_doi |
https://doi.org/10.1371/journal.pntd.0005898 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
10 |
container_start_page |
e0005898 |
_version_ |
1766342201175965696 |