ISOLATION AND CHARACTERIZATION OF COLLAGEN FROM SALMON SKIN (Salmo salar) USING ACID METHOD
Collagen is a type of protein that is plentiful and has a wide variety of applications. Collagen is often derived from porcine and bovine, however, both sources are considered to transmit disease, particularly porcine sources, which haram to Moslem. There are some methods for collagen extraction, on...
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Kementerian Kelautan dan Perikanan
2023
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ftdoajarticles:oai:doaj.org/article:b55b3bed7c794b98bb6d8d12518208fb 2023-10-01T03:59:14+02:00 ISOLATION AND CHARACTERIZATION OF COLLAGEN FROM SALMON SKIN (Salmo salar) USING ACID METHOD Amalia Afifah Ono Suparno Liesbetini Haditjaroko Kustiariah Tarman Agus Setiyono Aditya Wahyu Nugraha 2023-08-01T00:00:00Z https://doi.org/10.15578/squalen.756 https://doaj.org/article/b55b3bed7c794b98bb6d8d12518208fb EN eng Kementerian Kelautan dan Perikanan https://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/756 https://doaj.org/toc/2089-5690 https://doaj.org/toc/2406-9272 2089-5690 2406-9272 doi:10.15578/squalen.756 https://doaj.org/article/b55b3bed7c794b98bb6d8d12518208fb Squalen, Vol 18, Iss 2 (2023) acid extraction method collagen pretreatment salmon skin Ocean engineering TC1501-1800 Naval Science V article 2023 ftdoajarticles https://doi.org/10.15578/squalen.756 2023-09-03T00:49:47Z Collagen is a type of protein that is plentiful and has a wide variety of applications. Collagen is often derived from porcine and bovine, however, both sources are considered to transmit disease, particularly porcine sources, which haram to Moslem. There are some methods for collagen extraction, on of them using acid method. The purpose of this study was to obtain the best method of extraction and analyze collagen from alternative sources, specifically salmon skin, which is abundant. This study included two stages, i.e. pretreatment with sodium hydroxide for eliminating non-collagen proteins prior from salmon skin and extraction with acetic acid for collagen extraction. Pretreatment was carried out utilizing a split-plot design with parameters for determining the levels of non-collagen protein. The extraction step used a factorial completely randomized design to observe yields, amino acids, functional groups, and molecular weights. The results indicated that the extract was collagen. It was indicated by high glycine, alanine, and proline amino acid content. The result of SDS-PAGE showed the extracted was type I collagen was characterized by two identical 1 chains and one similar, but 2 different chains. The propeptides of type I procollagen have a similar composition. The most effective method for eliminating non-collagen proteins prior to extracting collagen from salmon skin was pretreatment with a 0.05 M NaOH solution for 10 hours. Collagen extraction using acetic acid at concentration of 0.5 M for 48 hours resulted in a yield of 12.79%. Article in Journal/Newspaper Salmo salar Directory of Open Access Journals: DOAJ Articles Haram ENVELOPE(6.445,6.445,62.599,62.599) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
acid extraction method collagen pretreatment salmon skin Ocean engineering TC1501-1800 Naval Science V |
spellingShingle |
acid extraction method collagen pretreatment salmon skin Ocean engineering TC1501-1800 Naval Science V Amalia Afifah Ono Suparno Liesbetini Haditjaroko Kustiariah Tarman Agus Setiyono Aditya Wahyu Nugraha ISOLATION AND CHARACTERIZATION OF COLLAGEN FROM SALMON SKIN (Salmo salar) USING ACID METHOD |
topic_facet |
acid extraction method collagen pretreatment salmon skin Ocean engineering TC1501-1800 Naval Science V |
description |
Collagen is a type of protein that is plentiful and has a wide variety of applications. Collagen is often derived from porcine and bovine, however, both sources are considered to transmit disease, particularly porcine sources, which haram to Moslem. There are some methods for collagen extraction, on of them using acid method. The purpose of this study was to obtain the best method of extraction and analyze collagen from alternative sources, specifically salmon skin, which is abundant. This study included two stages, i.e. pretreatment with sodium hydroxide for eliminating non-collagen proteins prior from salmon skin and extraction with acetic acid for collagen extraction. Pretreatment was carried out utilizing a split-plot design with parameters for determining the levels of non-collagen protein. The extraction step used a factorial completely randomized design to observe yields, amino acids, functional groups, and molecular weights. The results indicated that the extract was collagen. It was indicated by high glycine, alanine, and proline amino acid content. The result of SDS-PAGE showed the extracted was type I collagen was characterized by two identical 1 chains and one similar, but 2 different chains. The propeptides of type I procollagen have a similar composition. The most effective method for eliminating non-collagen proteins prior to extracting collagen from salmon skin was pretreatment with a 0.05 M NaOH solution for 10 hours. Collagen extraction using acetic acid at concentration of 0.5 M for 48 hours resulted in a yield of 12.79%. |
format |
Article in Journal/Newspaper |
author |
Amalia Afifah Ono Suparno Liesbetini Haditjaroko Kustiariah Tarman Agus Setiyono Aditya Wahyu Nugraha |
author_facet |
Amalia Afifah Ono Suparno Liesbetini Haditjaroko Kustiariah Tarman Agus Setiyono Aditya Wahyu Nugraha |
author_sort |
Amalia Afifah |
title |
ISOLATION AND CHARACTERIZATION OF COLLAGEN FROM SALMON SKIN (Salmo salar) USING ACID METHOD |
title_short |
ISOLATION AND CHARACTERIZATION OF COLLAGEN FROM SALMON SKIN (Salmo salar) USING ACID METHOD |
title_full |
ISOLATION AND CHARACTERIZATION OF COLLAGEN FROM SALMON SKIN (Salmo salar) USING ACID METHOD |
title_fullStr |
ISOLATION AND CHARACTERIZATION OF COLLAGEN FROM SALMON SKIN (Salmo salar) USING ACID METHOD |
title_full_unstemmed |
ISOLATION AND CHARACTERIZATION OF COLLAGEN FROM SALMON SKIN (Salmo salar) USING ACID METHOD |
title_sort |
isolation and characterization of collagen from salmon skin (salmo salar) using acid method |
publisher |
Kementerian Kelautan dan Perikanan |
publishDate |
2023 |
url |
https://doi.org/10.15578/squalen.756 https://doaj.org/article/b55b3bed7c794b98bb6d8d12518208fb |
long_lat |
ENVELOPE(6.445,6.445,62.599,62.599) |
geographic |
Haram |
geographic_facet |
Haram |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Squalen, Vol 18, Iss 2 (2023) |
op_relation |
https://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/756 https://doaj.org/toc/2089-5690 https://doaj.org/toc/2406-9272 2089-5690 2406-9272 doi:10.15578/squalen.756 https://doaj.org/article/b55b3bed7c794b98bb6d8d12518208fb |
op_doi |
https://doi.org/10.15578/squalen.756 |
_version_ |
1778532921809829888 |