Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells
Tekupalli Ravikiran,1 Santosh Anand,2 Mohammad Azam Ansari,3 Mohammad N Alomary,4 Sami AlYahya,4 Sowbhagya Ramachandregowda,1 Saad Alghamdi,5 Anupama Sindhghatta Kariyappa,1 Bhagyalakshmi Dundaiah,1 Mamatha Madhugiri Gopinath,1 Sumreen Sultana,1 Shital Manohar Punekar,1 Thimappa Ramachandrappa Laksh...
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Dove Medical Press
2021
|
Subjects: | |
Online Access: | https://doaj.org/article/b51fbc8dd1934ec1a6cc5e418646118e |
id |
ftdoajarticles:oai:doaj.org/article:b51fbc8dd1934ec1a6cc5e418646118e |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:b51fbc8dd1934ec1a6cc5e418646118e 2023-05-15T18:13:11+02:00 Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells Ravikiran T Anand S Ansari MA Alomary MN AlYahya S Ramachandregowda S Alghamdi S Sindhghatta Kariyappa A Dundaiah B Madhugiri Gopinath M Sultana S Punekar SM Lakshmeesha TR 2021-08-01T00:00:00Z https://doaj.org/article/b51fbc8dd1934ec1a6cc5e418646118e EN eng Dove Medical Press https://www.dovepress.com/fabrication-and-in-vitro-evaluation-of-4-hia-encapsulated-plga-nanopar-peer-reviewed-fulltext-article-IJN https://doaj.org/toc/1178-2013 1178-2013 https://doaj.org/article/b51fbc8dd1934ec1a6cc5e418646118e International Journal of Nanomedicine, Vol Volume 16, Pp 5621-5632 (2021) 4-hydroxyisopthalic acid polylactic-co-glycolic acid nanoencapsulation pc12 cells cytotoxicity Medicine (General) R5-920 article 2021 ftdoajarticles 2022-12-31T05:21:22Z Tekupalli Ravikiran,1 Santosh Anand,2 Mohammad Azam Ansari,3 Mohammad N Alomary,4 Sami AlYahya,4 Sowbhagya Ramachandregowda,1 Saad Alghamdi,5 Anupama Sindhghatta Kariyappa,1 Bhagyalakshmi Dundaiah,1 Mamatha Madhugiri Gopinath,1 Sumreen Sultana,1 Shital Manohar Punekar,1 Thimappa Ramachandrappa Lakshmeesha1 1Department of Biotechnology, Bangalore University, Bangalore, India; 2Department of Biotechnology, School of Applied Sciences, Reva University, Bengaluru, India; 3Department of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, 31441, Saudi Arabia; 4National Center for Biotechnology, Life Science and Environmental Research Institute, King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia; 5Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi ArabiaCorrespondence: Thimappa Ramachandrappa LakshmeeshaDepartment of Biotechnology, Bangalore University, Bangalore, 560056, Karnataka, IndiaTel +91 8710937464Email lakshmeeshat6@gmail.comMohammad Azam AnsariDepartment of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, 31441, Saudi ArabiaTel +966 580306835Email maansari@iau.edu.saPurpose: 4-Hydroxyisophthalic acid (4-HIA) is a bioactive compound present in the roots of Decalepis hamiltonii, which has attracted considerable attention in attenuating oxidative stress-related neurodegenerative diseases. However, its efficacy is limited because of its low solubility and bioavailability. Therefore, the present study aimed to encapsulate 4-HIA using biocompatible copolymer polylactide-co-glycolide (PLGA) and evaluate its antioxidant and neuroprotective potential.Methods: The nanoparticles (NPs) were fabricated by solid/oil/water (s/o/w) emulsion technique and characterized using XRD, SEM, HR-TEM, and FTIR spectroscopy. Antioxidant assays such as 1,1 diphenyl-2-picrylhydrazyl (DPPH), superoxide, ... Article in Journal/Newspaper sami Directory of Open Access Journals: DOAJ Articles Reva ENVELOPE(13.298,13.298,65.723,65.723) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
4-hydroxyisopthalic acid polylactic-co-glycolic acid nanoencapsulation pc12 cells cytotoxicity Medicine (General) R5-920 |
spellingShingle |
4-hydroxyisopthalic acid polylactic-co-glycolic acid nanoencapsulation pc12 cells cytotoxicity Medicine (General) R5-920 Ravikiran T Anand S Ansari MA Alomary MN AlYahya S Ramachandregowda S Alghamdi S Sindhghatta Kariyappa A Dundaiah B Madhugiri Gopinath M Sultana S Punekar SM Lakshmeesha TR Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells |
topic_facet |
4-hydroxyisopthalic acid polylactic-co-glycolic acid nanoencapsulation pc12 cells cytotoxicity Medicine (General) R5-920 |
description |
Tekupalli Ravikiran,1 Santosh Anand,2 Mohammad Azam Ansari,3 Mohammad N Alomary,4 Sami AlYahya,4 Sowbhagya Ramachandregowda,1 Saad Alghamdi,5 Anupama Sindhghatta Kariyappa,1 Bhagyalakshmi Dundaiah,1 Mamatha Madhugiri Gopinath,1 Sumreen Sultana,1 Shital Manohar Punekar,1 Thimappa Ramachandrappa Lakshmeesha1 1Department of Biotechnology, Bangalore University, Bangalore, India; 2Department of Biotechnology, School of Applied Sciences, Reva University, Bengaluru, India; 3Department of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, 31441, Saudi Arabia; 4National Center for Biotechnology, Life Science and Environmental Research Institute, King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia; 5Laboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi ArabiaCorrespondence: Thimappa Ramachandrappa LakshmeeshaDepartment of Biotechnology, Bangalore University, Bangalore, 560056, Karnataka, IndiaTel +91 8710937464Email lakshmeeshat6@gmail.comMohammad Azam AnsariDepartment of Epidemic Disease Research, Institutes for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, 31441, Saudi ArabiaTel +966 580306835Email maansari@iau.edu.saPurpose: 4-Hydroxyisophthalic acid (4-HIA) is a bioactive compound present in the roots of Decalepis hamiltonii, which has attracted considerable attention in attenuating oxidative stress-related neurodegenerative diseases. However, its efficacy is limited because of its low solubility and bioavailability. Therefore, the present study aimed to encapsulate 4-HIA using biocompatible copolymer polylactide-co-glycolide (PLGA) and evaluate its antioxidant and neuroprotective potential.Methods: The nanoparticles (NPs) were fabricated by solid/oil/water (s/o/w) emulsion technique and characterized using XRD, SEM, HR-TEM, and FTIR spectroscopy. Antioxidant assays such as 1,1 diphenyl-2-picrylhydrazyl (DPPH), superoxide, ... |
format |
Article in Journal/Newspaper |
author |
Ravikiran T Anand S Ansari MA Alomary MN AlYahya S Ramachandregowda S Alghamdi S Sindhghatta Kariyappa A Dundaiah B Madhugiri Gopinath M Sultana S Punekar SM Lakshmeesha TR |
author_facet |
Ravikiran T Anand S Ansari MA Alomary MN AlYahya S Ramachandregowda S Alghamdi S Sindhghatta Kariyappa A Dundaiah B Madhugiri Gopinath M Sultana S Punekar SM Lakshmeesha TR |
author_sort |
Ravikiran T |
title |
Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells |
title_short |
Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells |
title_full |
Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells |
title_fullStr |
Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells |
title_full_unstemmed |
Fabrication and in vitro Evaluation of 4-HIA Encapsulated PLGA Nanoparticles on PC12 Cells |
title_sort |
fabrication and in vitro evaluation of 4-hia encapsulated plga nanoparticles on pc12 cells |
publisher |
Dove Medical Press |
publishDate |
2021 |
url |
https://doaj.org/article/b51fbc8dd1934ec1a6cc5e418646118e |
long_lat |
ENVELOPE(13.298,13.298,65.723,65.723) |
geographic |
Reva |
geographic_facet |
Reva |
genre |
sami |
genre_facet |
sami |
op_source |
International Journal of Nanomedicine, Vol Volume 16, Pp 5621-5632 (2021) |
op_relation |
https://www.dovepress.com/fabrication-and-in-vitro-evaluation-of-4-hia-encapsulated-plga-nanopar-peer-reviewed-fulltext-article-IJN https://doaj.org/toc/1178-2013 1178-2013 https://doaj.org/article/b51fbc8dd1934ec1a6cc5e418646118e |
_version_ |
1766185679540191232 |