Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA.
Melioidosis is caused by Burkholderia pseudomallei, a Gram-negative bacillus, primarily found in soils in Southeast Asia and northern Australia. A recent case of melioidosis in non-endemic Arizona was determined to be the result of locally acquired infection, as the patient had no travel history to...
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ftdoajarticles:oai:doaj.org/article:b4cd181981cb4f4cb3b6ef82902e7177 2023-05-15T15:02:51+02:00 Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA. David M Engelthaler Jolene Bowers James A Schupp Talima Pearson Jennifer Ginther Heidie M Hornstra Julia Dale Tasha Stewart Rebecca Sunenshine Victor Waddell Craig Levy John Gillece Lance B Price Tania Contente Stephen M Beckstrom-Sternberg David D Blaney David M Wagner Mark Mayo Bart J Currie Paul Keim Apichai Tuanyok 2011-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001347 https://doaj.org/article/b4cd181981cb4f4cb3b6ef82902e7177 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3196475?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001347 https://doaj.org/article/b4cd181981cb4f4cb3b6ef82902e7177 PLoS Neglected Tropical Diseases, Vol 5, Iss 10, p e1347 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001347 2022-12-31T13:04:09Z Melioidosis is caused by Burkholderia pseudomallei, a Gram-negative bacillus, primarily found in soils in Southeast Asia and northern Australia. A recent case of melioidosis in non-endemic Arizona was determined to be the result of locally acquired infection, as the patient had no travel history to endemic regions and no previous history of disease. Diagnosis of the case was confirmed through multiple microbiologic and molecular techniques. To enhance the epidemiological analysis, we conducted several molecular genotyping procedures, including multi-locus sequence typing, SNP-profiling, and whole genome sequence typing. Each technique has different molecular epidemiologic advantages, all of which provided evidence that the infecting strain was most similar to those found in Southeast Asia, possibly originating in, or around, Malaysia. Advancements in new typing technologies provide genotyping resolution not previously available to public health investigators, allowing for more accurate source identification. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 10 e1347 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 David M Engelthaler Jolene Bowers James A Schupp Talima Pearson Jennifer Ginther Heidie M Hornstra Julia Dale Tasha Stewart Rebecca Sunenshine Victor Waddell Craig Levy John Gillece Lance B Price Tania Contente Stephen M Beckstrom-Sternberg David D Blaney David M Wagner Mark Mayo Bart J Currie Paul Keim Apichai Tuanyok Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Melioidosis is caused by Burkholderia pseudomallei, a Gram-negative bacillus, primarily found in soils in Southeast Asia and northern Australia. A recent case of melioidosis in non-endemic Arizona was determined to be the result of locally acquired infection, as the patient had no travel history to endemic regions and no previous history of disease. Diagnosis of the case was confirmed through multiple microbiologic and molecular techniques. To enhance the epidemiological analysis, we conducted several molecular genotyping procedures, including multi-locus sequence typing, SNP-profiling, and whole genome sequence typing. Each technique has different molecular epidemiologic advantages, all of which provided evidence that the infecting strain was most similar to those found in Southeast Asia, possibly originating in, or around, Malaysia. Advancements in new typing technologies provide genotyping resolution not previously available to public health investigators, allowing for more accurate source identification. |
format |
Article in Journal/Newspaper |
author |
David M Engelthaler Jolene Bowers James A Schupp Talima Pearson Jennifer Ginther Heidie M Hornstra Julia Dale Tasha Stewart Rebecca Sunenshine Victor Waddell Craig Levy John Gillece Lance B Price Tania Contente Stephen M Beckstrom-Sternberg David D Blaney David M Wagner Mark Mayo Bart J Currie Paul Keim Apichai Tuanyok |
author_facet |
David M Engelthaler Jolene Bowers James A Schupp Talima Pearson Jennifer Ginther Heidie M Hornstra Julia Dale Tasha Stewart Rebecca Sunenshine Victor Waddell Craig Levy John Gillece Lance B Price Tania Contente Stephen M Beckstrom-Sternberg David D Blaney David M Wagner Mark Mayo Bart J Currie Paul Keim Apichai Tuanyok |
author_sort |
David M Engelthaler |
title |
Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA. |
title_short |
Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA. |
title_full |
Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA. |
title_fullStr |
Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA. |
title_full_unstemmed |
Molecular investigations of a locally acquired case of melioidosis in Southern AZ, USA. |
title_sort |
molecular investigations of a locally acquired case of melioidosis in southern az, usa. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001347 https://doaj.org/article/b4cd181981cb4f4cb3b6ef82902e7177 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 10, p e1347 (2011) |
op_relation |
http://europepmc.org/articles/PMC3196475?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001347 https://doaj.org/article/b4cd181981cb4f4cb3b6ef82902e7177 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001347 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
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10 |
container_start_page |
e1347 |
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