Dried Blood Spots (DBS): A suitable alternative to using whole blood samples for diagnostic testing of visceral leishmaniasis in the post-elimination era.
Background Serum or whole blood collection, processing, transport and storage still present significant challenges in low resource settings where mass surveillance is required to sustain disease elimination. Therefore, in this study, we explored the diagnostic efficacy of dried blood spots (DBS) as...
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ftdoajarticles:oai:doaj.org/article:b49313556dd141ddb3ba5df9b42638a4 2023-12-03T10:18:21+01:00 Dried Blood Spots (DBS): A suitable alternative to using whole blood samples for diagnostic testing of visceral leishmaniasis in the post-elimination era. Prakash Ghosh Rajashree Chowdhury Md Abu Rahat Faria Hossain Nur E Arpha Mojca Kristan Matthew Higgins Ahmed Abd El Wahed Yasuyuki Goto M M Towhidul Islam Susana Campino Mary Cameron Malcom S Duthie Rashidul Haque Dinesh Mondal 2023-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011680 https://doaj.org/article/b49313556dd141ddb3ba5df9b42638a4 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011680 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011680 https://doaj.org/article/b49313556dd141ddb3ba5df9b42638a4 PLoS Neglected Tropical Diseases, Vol 17, Iss 10, p e0011680 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011680 2023-11-05T01:37:23Z Background Serum or whole blood collection, processing, transport and storage still present significant challenges in low resource settings where mass surveillance is required to sustain disease elimination. Therefore, in this study, we explored the diagnostic efficacy of dried blood spots (DBS) as a minimally invasive and potentially cost-effective alternative sampling technique to whole blood sampling procedures for subsequent detection of Leishmania donovani antibodies or DNA. Methodology and principal findings Archived serum, DNA samples from whole blood of visceral leishmaniasis (VL) cases and healthy controls, and DBS from corresponding cases and controls, were used. Both molecular and serological assays were optimized to detect L. donovani antibodies or DNA in DBS elute and results were compared against those obtained with whole blood. Serological assays (both rK28 ELISA and rK39 ELISA) of DBS samples showed sensitivity and specificity of 100% and had excellent agreement with results from whole blood samples (kappa value ranged from 0.98-1). Bland-Altman analysis of OD values from rK28-ELISA with DBS elute and patients' serum showed an excellent agreement (ICC = 0.9) whereas a good agreement (ICC = 0.8) was observed in the case of rK39-ELISA. However, qPCR and RPA of DBS samples had a diminished sensitivity of 76% and 68%, respectively, and poor agreement was observed with the whole blood samples. Conclusion Our results demonstrate that DBS offer excellent diagnostic efficiency for serological assays and represent a viable alternative to whole blood sampling procedures. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 17 10 e0011680 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Prakash Ghosh Rajashree Chowdhury Md Abu Rahat Faria Hossain Nur E Arpha Mojca Kristan Matthew Higgins Ahmed Abd El Wahed Yasuyuki Goto M M Towhidul Islam Susana Campino Mary Cameron Malcom S Duthie Rashidul Haque Dinesh Mondal Dried Blood Spots (DBS): A suitable alternative to using whole blood samples for diagnostic testing of visceral leishmaniasis in the post-elimination era. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Serum or whole blood collection, processing, transport and storage still present significant challenges in low resource settings where mass surveillance is required to sustain disease elimination. Therefore, in this study, we explored the diagnostic efficacy of dried blood spots (DBS) as a minimally invasive and potentially cost-effective alternative sampling technique to whole blood sampling procedures for subsequent detection of Leishmania donovani antibodies or DNA. Methodology and principal findings Archived serum, DNA samples from whole blood of visceral leishmaniasis (VL) cases and healthy controls, and DBS from corresponding cases and controls, were used. Both molecular and serological assays were optimized to detect L. donovani antibodies or DNA in DBS elute and results were compared against those obtained with whole blood. Serological assays (both rK28 ELISA and rK39 ELISA) of DBS samples showed sensitivity and specificity of 100% and had excellent agreement with results from whole blood samples (kappa value ranged from 0.98-1). Bland-Altman analysis of OD values from rK28-ELISA with DBS elute and patients' serum showed an excellent agreement (ICC = 0.9) whereas a good agreement (ICC = 0.8) was observed in the case of rK39-ELISA. However, qPCR and RPA of DBS samples had a diminished sensitivity of 76% and 68%, respectively, and poor agreement was observed with the whole blood samples. Conclusion Our results demonstrate that DBS offer excellent diagnostic efficiency for serological assays and represent a viable alternative to whole blood sampling procedures. |
format |
Article in Journal/Newspaper |
author |
Prakash Ghosh Rajashree Chowdhury Md Abu Rahat Faria Hossain Nur E Arpha Mojca Kristan Matthew Higgins Ahmed Abd El Wahed Yasuyuki Goto M M Towhidul Islam Susana Campino Mary Cameron Malcom S Duthie Rashidul Haque Dinesh Mondal |
author_facet |
Prakash Ghosh Rajashree Chowdhury Md Abu Rahat Faria Hossain Nur E Arpha Mojca Kristan Matthew Higgins Ahmed Abd El Wahed Yasuyuki Goto M M Towhidul Islam Susana Campino Mary Cameron Malcom S Duthie Rashidul Haque Dinesh Mondal |
author_sort |
Prakash Ghosh |
title |
Dried Blood Spots (DBS): A suitable alternative to using whole blood samples for diagnostic testing of visceral leishmaniasis in the post-elimination era. |
title_short |
Dried Blood Spots (DBS): A suitable alternative to using whole blood samples for diagnostic testing of visceral leishmaniasis in the post-elimination era. |
title_full |
Dried Blood Spots (DBS): A suitable alternative to using whole blood samples for diagnostic testing of visceral leishmaniasis in the post-elimination era. |
title_fullStr |
Dried Blood Spots (DBS): A suitable alternative to using whole blood samples for diagnostic testing of visceral leishmaniasis in the post-elimination era. |
title_full_unstemmed |
Dried Blood Spots (DBS): A suitable alternative to using whole blood samples for diagnostic testing of visceral leishmaniasis in the post-elimination era. |
title_sort |
dried blood spots (dbs): a suitable alternative to using whole blood samples for diagnostic testing of visceral leishmaniasis in the post-elimination era. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011680 https://doaj.org/article/b49313556dd141ddb3ba5df9b42638a4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 10, p e0011680 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011680 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011680 https://doaj.org/article/b49313556dd141ddb3ba5df9b42638a4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0011680 |
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PLOS Neglected Tropical Diseases |
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17 |
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10 |
container_start_page |
e0011680 |
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