A symbiotic gut bacterium enhances Aedes albopictus resistance to insecticide.
Background The increasing insecticide resistance of Aedes albopictus puts many countries in Asia and Africa, including China, at great risk of a mosquito-borne virus epidemic. To date, a growing number of researches have focused on the relationship between intestinal symbiotic bacteria and their hos...
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ftdoajarticles:oai:doaj.org/article:b48de18087d24a1e8507dc04a3424dd5 2023-05-15T15:15:07+02:00 A symbiotic gut bacterium enhances Aedes albopictus resistance to insecticide. Haiyang Wang Hongmei Liu Hui Peng Yang Wang Chongxing Zhang Xiuxia Guo Haifang Wang Lijuan Liu Wenxiang Lv Peng Cheng Maoqing Gong 2022-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010208 https://doaj.org/article/b48de18087d24a1e8507dc04a3424dd5 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010208 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010208 https://doaj.org/article/b48de18087d24a1e8507dc04a3424dd5 PLoS Neglected Tropical Diseases, Vol 16, Iss 3, p e0010208 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010208 2022-12-31T00:35:18Z Background The increasing insecticide resistance of Aedes albopictus puts many countries in Asia and Africa, including China, at great risk of a mosquito-borne virus epidemic. To date, a growing number of researches have focused on the relationship between intestinal symbiotic bacteria and their hosts' resistance to insecticides. This provides a novel aspect to the study of resistant mechanisms. Methods/findings This study reveals significant composition and dynamic changes in the intestinal symbiotic bacteria of Ae. albopictus between the resistant and susceptible strains based on full-length sequencing technology. The relative abundance of Serratia oryzae was significantly higher in the resistance strain than in the susceptible strains; also, the relative abundance of S. oryzae was significantly higher in deltamethrin-induced Ae. albopictus than in their counterpart. These suggested that S. oryzae may be involved in the development of insecticide resistance in Ae. albopictus. To explore the insecticide resistance mechanism, adult mosquitoes were fed with GFP-tagged S. oryzae, which resulted in stable bacterial enrichment in the mosquito gut without affecting the normal physiology, longevity, oviposition, and hatching rates of the host. The resistance measurements were made based on bioassays as per the WHO guidelines. The results showed that the survival rate of S. oryzae-enriched Ae. albopictus was significantly higher than the untreated mosquitoes, indicating the enhanced resistance of S. oryzae-enriched Ae. albopictus. Also, the activities of three metabolic detoxification enzymes in S. oryzae-enriched mosquitoes were increased to varying degrees. Meanwhile, the activity of extracellular enzymes released by S. oryzae was measured, but only carboxylesterase activity was detected. HPLC and UHPLC were respectively used to measure deltamethrin residue concentration and metabolite qualitative analysis, showing that the deltamethrin degradation efficiency of S. oryzae was positively correlated with time and ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 3 e0010208 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Haiyang Wang Hongmei Liu Hui Peng Yang Wang Chongxing Zhang Xiuxia Guo Haifang Wang Lijuan Liu Wenxiang Lv Peng Cheng Maoqing Gong A symbiotic gut bacterium enhances Aedes albopictus resistance to insecticide. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background The increasing insecticide resistance of Aedes albopictus puts many countries in Asia and Africa, including China, at great risk of a mosquito-borne virus epidemic. To date, a growing number of researches have focused on the relationship between intestinal symbiotic bacteria and their hosts' resistance to insecticides. This provides a novel aspect to the study of resistant mechanisms. Methods/findings This study reveals significant composition and dynamic changes in the intestinal symbiotic bacteria of Ae. albopictus between the resistant and susceptible strains based on full-length sequencing technology. The relative abundance of Serratia oryzae was significantly higher in the resistance strain than in the susceptible strains; also, the relative abundance of S. oryzae was significantly higher in deltamethrin-induced Ae. albopictus than in their counterpart. These suggested that S. oryzae may be involved in the development of insecticide resistance in Ae. albopictus. To explore the insecticide resistance mechanism, adult mosquitoes were fed with GFP-tagged S. oryzae, which resulted in stable bacterial enrichment in the mosquito gut without affecting the normal physiology, longevity, oviposition, and hatching rates of the host. The resistance measurements were made based on bioassays as per the WHO guidelines. The results showed that the survival rate of S. oryzae-enriched Ae. albopictus was significantly higher than the untreated mosquitoes, indicating the enhanced resistance of S. oryzae-enriched Ae. albopictus. Also, the activities of three metabolic detoxification enzymes in S. oryzae-enriched mosquitoes were increased to varying degrees. Meanwhile, the activity of extracellular enzymes released by S. oryzae was measured, but only carboxylesterase activity was detected. HPLC and UHPLC were respectively used to measure deltamethrin residue concentration and metabolite qualitative analysis, showing that the deltamethrin degradation efficiency of S. oryzae was positively correlated with time and ... |
format |
Article in Journal/Newspaper |
author |
Haiyang Wang Hongmei Liu Hui Peng Yang Wang Chongxing Zhang Xiuxia Guo Haifang Wang Lijuan Liu Wenxiang Lv Peng Cheng Maoqing Gong |
author_facet |
Haiyang Wang Hongmei Liu Hui Peng Yang Wang Chongxing Zhang Xiuxia Guo Haifang Wang Lijuan Liu Wenxiang Lv Peng Cheng Maoqing Gong |
author_sort |
Haiyang Wang |
title |
A symbiotic gut bacterium enhances Aedes albopictus resistance to insecticide. |
title_short |
A symbiotic gut bacterium enhances Aedes albopictus resistance to insecticide. |
title_full |
A symbiotic gut bacterium enhances Aedes albopictus resistance to insecticide. |
title_fullStr |
A symbiotic gut bacterium enhances Aedes albopictus resistance to insecticide. |
title_full_unstemmed |
A symbiotic gut bacterium enhances Aedes albopictus resistance to insecticide. |
title_sort |
symbiotic gut bacterium enhances aedes albopictus resistance to insecticide. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010208 https://doaj.org/article/b48de18087d24a1e8507dc04a3424dd5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 3, p e0010208 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010208 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010208 https://doaj.org/article/b48de18087d24a1e8507dc04a3424dd5 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010208 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
3 |
container_start_page |
e0010208 |
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1766345504111722496 |