Phylogenetic Analysis of Dengue Virus in Bangkalan, Madura Island, East Java Province, Indonesia
Dengue virus (DENV) infection is a major health issue in tropical and subtropical areas. Indonesia is one of the biggest dengue endemic countries in the world. In the present study, the phylogenetic analysis of DENV in Bangkalan, Madura Island, Indonesia, was performed in order to obtain a clearer u...
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ftdoajarticles:oai:doaj.org/article:b45f77a0f35f4aacb909493586cfbd35 2023-05-15T15:07:43+02:00 Phylogenetic Analysis of Dengue Virus in Bangkalan, Madura Island, East Java Province, Indonesia Teguh Hari Sucipto Tomohiro Kotaki Kris Cahyo Mulyatno Siti Churrotin Amaliah Labiqah Soegeng Soegijanto Masanori Kameoka 2018-01-01T00:00:00Z https://doi.org/10.1155/2018/8127093 https://doaj.org/article/b45f77a0f35f4aacb909493586cfbd35 EN eng Hindawi Limited http://dx.doi.org/10.1155/2018/8127093 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2018/8127093 https://doaj.org/article/b45f77a0f35f4aacb909493586cfbd35 Journal of Tropical Medicine, Vol 2018 (2018) Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.1155/2018/8127093 2022-12-31T03:52:47Z Dengue virus (DENV) infection is a major health issue in tropical and subtropical areas. Indonesia is one of the biggest dengue endemic countries in the world. In the present study, the phylogenetic analysis of DENV in Bangkalan, Madura Island, Indonesia, was performed in order to obtain a clearer understanding of its dynamics in this country. A total of 359 blood samples from dengue-suspected patients were collected between 2012 and 2014. Serotyping was conducted using a multiplex Reverse Transcriptase-Polymerase Chain Reaction and a phylogenetic analysis of E gene sequences was performed using the Bayesian Markov chain Monte Carlo (MCMC) method. 17 out of 359 blood samples (4.7%) were positive for the isolation of DENV. Serotyping and the phylogenetic analysis revealed the predominance of DENV-1 genotype I (9/17, 52.9%), followed by DENV-2 Cosmopolitan type (7/17, 41.2%) and DENV-3 genotype I (1/17, 5.9%). DENV-4 was not isolated. The Madura Island isolates showed high nucleotide similarity to other Indonesian isolates, indicating frequent virus circulation in Indonesia. The results of the present study highlight the importance of continuous viral surveillance in dengue endemic areas in order to obtain a clearer understanding of the dynamics of DENV in Indonesia. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2018 1 6 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 |
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Arctic medicine. Tropical medicine RC955-962 Teguh Hari Sucipto Tomohiro Kotaki Kris Cahyo Mulyatno Siti Churrotin Amaliah Labiqah Soegeng Soegijanto Masanori Kameoka Phylogenetic Analysis of Dengue Virus in Bangkalan, Madura Island, East Java Province, Indonesia |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
Dengue virus (DENV) infection is a major health issue in tropical and subtropical areas. Indonesia is one of the biggest dengue endemic countries in the world. In the present study, the phylogenetic analysis of DENV in Bangkalan, Madura Island, Indonesia, was performed in order to obtain a clearer understanding of its dynamics in this country. A total of 359 blood samples from dengue-suspected patients were collected between 2012 and 2014. Serotyping was conducted using a multiplex Reverse Transcriptase-Polymerase Chain Reaction and a phylogenetic analysis of E gene sequences was performed using the Bayesian Markov chain Monte Carlo (MCMC) method. 17 out of 359 blood samples (4.7%) were positive for the isolation of DENV. Serotyping and the phylogenetic analysis revealed the predominance of DENV-1 genotype I (9/17, 52.9%), followed by DENV-2 Cosmopolitan type (7/17, 41.2%) and DENV-3 genotype I (1/17, 5.9%). DENV-4 was not isolated. The Madura Island isolates showed high nucleotide similarity to other Indonesian isolates, indicating frequent virus circulation in Indonesia. The results of the present study highlight the importance of continuous viral surveillance in dengue endemic areas in order to obtain a clearer understanding of the dynamics of DENV in Indonesia. |
format |
Article in Journal/Newspaper |
author |
Teguh Hari Sucipto Tomohiro Kotaki Kris Cahyo Mulyatno Siti Churrotin Amaliah Labiqah Soegeng Soegijanto Masanori Kameoka |
author_facet |
Teguh Hari Sucipto Tomohiro Kotaki Kris Cahyo Mulyatno Siti Churrotin Amaliah Labiqah Soegeng Soegijanto Masanori Kameoka |
author_sort |
Teguh Hari Sucipto |
title |
Phylogenetic Analysis of Dengue Virus in Bangkalan, Madura Island, East Java Province, Indonesia |
title_short |
Phylogenetic Analysis of Dengue Virus in Bangkalan, Madura Island, East Java Province, Indonesia |
title_full |
Phylogenetic Analysis of Dengue Virus in Bangkalan, Madura Island, East Java Province, Indonesia |
title_fullStr |
Phylogenetic Analysis of Dengue Virus in Bangkalan, Madura Island, East Java Province, Indonesia |
title_full_unstemmed |
Phylogenetic Analysis of Dengue Virus in Bangkalan, Madura Island, East Java Province, Indonesia |
title_sort |
phylogenetic analysis of dengue virus in bangkalan, madura island, east java province, indonesia |
publisher |
Hindawi Limited |
publishDate |
2018 |
url |
https://doi.org/10.1155/2018/8127093 https://doaj.org/article/b45f77a0f35f4aacb909493586cfbd35 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2018 (2018) |
op_relation |
http://dx.doi.org/10.1155/2018/8127093 https://doaj.org/toc/1687-9686 https://doaj.org/toc/1687-9694 1687-9686 1687-9694 doi:10.1155/2018/8127093 https://doaj.org/article/b45f77a0f35f4aacb909493586cfbd35 |
op_doi |
https://doi.org/10.1155/2018/8127093 |
container_title |
Journal of Tropical Medicine |
container_volume |
2018 |
container_start_page |
1 |
op_container_end_page |
6 |
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1766339150941782016 |