The effect of a single blood meal on the phenotypic expression of insecticide resistance in the major malaria vector Anopheles funestus
Abstract Background Anopheles funestus is a major malaria vector in southern Africa. Vector control relies on the use of insecticide chemicals to significantly reduce the number of malaria vectors by targeting that portion of the female population that takes blood meals and subsequently rests indoor...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2008
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1475-2875-7-226 https://doaj.org/article/b451ea00c1a3494fa58ab8c876213f78 |
id |
ftdoajarticles:oai:doaj.org/article:b451ea00c1a3494fa58ab8c876213f78 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:b451ea00c1a3494fa58ab8c876213f78 2023-05-15T15:16:13+02:00 The effect of a single blood meal on the phenotypic expression of insecticide resistance in the major malaria vector Anopheles funestus Coetzee Maureen Spillings Belinda L Koekemoer Lizette L Brooke Basil D 2008-10-01T00:00:00Z https://doi.org/10.1186/1475-2875-7-226 https://doaj.org/article/b451ea00c1a3494fa58ab8c876213f78 EN eng BMC http://www.malariajournal.com/content/7/1/226 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-226 1475-2875 https://doaj.org/article/b451ea00c1a3494fa58ab8c876213f78 Malaria Journal, Vol 7, Iss 1, p 226 (2008) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2008 ftdoajarticles https://doi.org/10.1186/1475-2875-7-226 2022-12-31T01:11:10Z Abstract Background Anopheles funestus is a major malaria vector in southern Africa. Vector control relies on the use of insecticide chemicals to significantly reduce the number of malaria vectors by targeting that portion of the female population that takes blood meals and subsequently rests indoors. It has been suggested that the intake of a blood meal may assist female mosquitoes to tolerate higher doses of insecticide through vigour tolerance. It is hypothesized that during the process of blood digestion, detoxification mechanisms required for the neutralizing of harmful components in the blood meal may also confer an increased ability to tolerate insecticide intoxication through increased enzyme regulation. Methods Bottle bioassays using a range of concentrations of the pyrethroid insecticide permethrin were performed on pyrethroid susceptible and resistant laboratory strains of An. funestus in order to detect differences in insecticide susceptibility following a single blood meal. Based on these results, a discriminating dosage was identified (double the lowest dosage that resulted in 100% mortality of the susceptible strain). Blood-fed and unfed females drawn from the resistant strain of An. funestus were then assayed against this discriminating dose, and the percentage mortality for each sample was scored and compared. Results In the insecticide dose response assays neither the fully susceptible nor the resistant strain of An. funestus showed any significant difference in insecticide susceptibility following a blood meal, regardless of the stage of blood meal digestion. A significant increase in the level of resistance was however detected in the resistant An. funestus strain following a single blood meal, based on exposure to a discriminating dose of permethrin. Conclusion The fully susceptible An. funestus strain did not show any significant alteration in susceptibility to insecticide following a blood meal suggesting that vigour tolerance through increased body mass (and increased dilution of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 7 1 226 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Coetzee Maureen Spillings Belinda L Koekemoer Lizette L Brooke Basil D The effect of a single blood meal on the phenotypic expression of insecticide resistance in the major malaria vector Anopheles funestus |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Anopheles funestus is a major malaria vector in southern Africa. Vector control relies on the use of insecticide chemicals to significantly reduce the number of malaria vectors by targeting that portion of the female population that takes blood meals and subsequently rests indoors. It has been suggested that the intake of a blood meal may assist female mosquitoes to tolerate higher doses of insecticide through vigour tolerance. It is hypothesized that during the process of blood digestion, detoxification mechanisms required for the neutralizing of harmful components in the blood meal may also confer an increased ability to tolerate insecticide intoxication through increased enzyme regulation. Methods Bottle bioassays using a range of concentrations of the pyrethroid insecticide permethrin were performed on pyrethroid susceptible and resistant laboratory strains of An. funestus in order to detect differences in insecticide susceptibility following a single blood meal. Based on these results, a discriminating dosage was identified (double the lowest dosage that resulted in 100% mortality of the susceptible strain). Blood-fed and unfed females drawn from the resistant strain of An. funestus were then assayed against this discriminating dose, and the percentage mortality for each sample was scored and compared. Results In the insecticide dose response assays neither the fully susceptible nor the resistant strain of An. funestus showed any significant difference in insecticide susceptibility following a blood meal, regardless of the stage of blood meal digestion. A significant increase in the level of resistance was however detected in the resistant An. funestus strain following a single blood meal, based on exposure to a discriminating dose of permethrin. Conclusion The fully susceptible An. funestus strain did not show any significant alteration in susceptibility to insecticide following a blood meal suggesting that vigour tolerance through increased body mass (and increased dilution of ... |
format |
Article in Journal/Newspaper |
author |
Coetzee Maureen Spillings Belinda L Koekemoer Lizette L Brooke Basil D |
author_facet |
Coetzee Maureen Spillings Belinda L Koekemoer Lizette L Brooke Basil D |
author_sort |
Coetzee Maureen |
title |
The effect of a single blood meal on the phenotypic expression of insecticide resistance in the major malaria vector Anopheles funestus |
title_short |
The effect of a single blood meal on the phenotypic expression of insecticide resistance in the major malaria vector Anopheles funestus |
title_full |
The effect of a single blood meal on the phenotypic expression of insecticide resistance in the major malaria vector Anopheles funestus |
title_fullStr |
The effect of a single blood meal on the phenotypic expression of insecticide resistance in the major malaria vector Anopheles funestus |
title_full_unstemmed |
The effect of a single blood meal on the phenotypic expression of insecticide resistance in the major malaria vector Anopheles funestus |
title_sort |
effect of a single blood meal on the phenotypic expression of insecticide resistance in the major malaria vector anopheles funestus |
publisher |
BMC |
publishDate |
2008 |
url |
https://doi.org/10.1186/1475-2875-7-226 https://doaj.org/article/b451ea00c1a3494fa58ab8c876213f78 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 7, Iss 1, p 226 (2008) |
op_relation |
http://www.malariajournal.com/content/7/1/226 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-226 1475-2875 https://doaj.org/article/b451ea00c1a3494fa58ab8c876213f78 |
op_doi |
https://doi.org/10.1186/1475-2875-7-226 |
container_title |
Malaria Journal |
container_volume |
7 |
container_issue |
1 |
container_start_page |
226 |
_version_ |
1766346508552110080 |