Quantitative analysis of γ–oryzanol content in cold pressed rice bran oil by TLC–image analysis method

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Apirak Sakunpak, Jirapornchai Suksaeree, Chaowalit Monton, Pathamaporn Pathompak, Krisana Kraisintu
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2014
• Subjects: γ–Oryzanol; TLC–densitometric method; TLC–image analysis method; Rice bran oil; Quantitative analysis; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/S2221-1691(14)60219-7; https://doaj.org/article/b43bcf5c838a4b58aeb70fb8c4182bc4

Objective: To develop and validate an image analysis method for quantitative analysis of γ-oryzanol in cold pressed rice bran oil. Methods: TLC-densitometric and TLC-image analysis methods were developed, validated, and used for quantitative analysis of γ-oryzanol in cold pressed rice bran oil. The results obtained by these two different quantification methods were compared by paired t-test. Results: Both assays provided good linearity, accuracy, reproducibility and selectivity for determination of γ-oryzanol. Conclusions: The TLC-densitometric and TLC-image analysis methods provided a similar reproducibility, accuracy and selectivity for the quantitative determination of γ-oryzanol in cold pressed rice bran oil. A statistical comparison of the quantitative determinations of γ-oryzanol in samples did not show any statistically significant difference between TLC-densitometric and TLC-image analysis methods. As both methods were found to be equal, they therefore can be used for the determination of γ-oryzanol in cold pressed rice bran oil.