High prevalence of the simultaneous excretion of polyomaviruses JC and BK in the urine of HIV-infected patients without neurological symptoms in São Paulo, Brazil
OBJECTIVE: To evaluate the prevalence of the urinary excretion of BKV and JCV in HIV-infected patients without neurological symptoms. METHODS: Urine samples from HIV-infected patients without neurological symptoms were tested for JC virus and BK virus by PCR. Samples were screened for the presence o...
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:b4081ae3a815454194a8cefd510e06fc 2024-09-09T19:26:32+00:00 High prevalence of the simultaneous excretion of polyomaviruses JC and BK in the urine of HIV-infected patients without neurological symptoms in São Paulo, Brazil Luiz Henrique da Silva Nali Cristiane de Campos Centrone Paulo Roberto Palma Urbano Augusto César Penalva-de-Oliveira Jose Ernesto Vidal Erique Peixoto Miranda Claudio Sérgio Pannuti Maria Cristina Domingues da Silva Fink 2012-08-01T00:00:00Z https://doi.org/10.1590/S0036-46652012000400004 https://doaj.org/article/b4081ae3a815454194a8cefd510e06fc EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652012000400004&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652012000400004 https://doaj.org/article/b4081ae3a815454194a8cefd510e06fc Revista do Instituto de Medicina Tropical de São Paulo, Vol 54, Iss 4, Pp 201-205 (2012) JC virus DNA BK virus DNA Simultaneous excretion HIV-infected Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1590/S0036-46652012000400004 2024-08-05T17:49:30Z OBJECTIVE: To evaluate the prevalence of the urinary excretion of BKV and JCV in HIV-infected patients without neurological symptoms. METHODS: Urine samples from HIV-infected patients without neurological symptoms were tested for JC virus and BK virus by PCR. Samples were screened for the presence of polyomavirus with sets of primers complementary to the early region of JCV and BKV genome (AgT). The presence of JC virus or BK virus were confirmed by two other PCR assays using sets of primers complementary to the VP1 gene of each virus. Analysis of the data was performed by the Kruskal-Wallis test for numerical data and Pearson or Yates for categorical variables. RESULTS: A total of 75 patients were included in the study. The overall prevalence of polyomavirus DNA urinary shedding was 67/75 (89.3%). Only BKV DNA was detected in 14/75 (18.7%) urine samples, and only JCV DNA was detected in 11/75 (14.7%) samples. Both BKV and JCV DNA were present in 42/75 (56.0%) samples. CONCLUSION: In this study we found high rates of excretion of JCV, BKV, and simultaneous excretion in HIV+ patients. Also these results differ from the others available on the literature. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 54 4 201 205 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
JC virus DNA BK virus DNA Simultaneous excretion HIV-infected Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
JC virus DNA BK virus DNA Simultaneous excretion HIV-infected Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Luiz Henrique da Silva Nali Cristiane de Campos Centrone Paulo Roberto Palma Urbano Augusto César Penalva-de-Oliveira Jose Ernesto Vidal Erique Peixoto Miranda Claudio Sérgio Pannuti Maria Cristina Domingues da Silva Fink High prevalence of the simultaneous excretion of polyomaviruses JC and BK in the urine of HIV-infected patients without neurological symptoms in São Paulo, Brazil |
topic_facet |
JC virus DNA BK virus DNA Simultaneous excretion HIV-infected Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
OBJECTIVE: To evaluate the prevalence of the urinary excretion of BKV and JCV in HIV-infected patients without neurological symptoms. METHODS: Urine samples from HIV-infected patients without neurological symptoms were tested for JC virus and BK virus by PCR. Samples were screened for the presence of polyomavirus with sets of primers complementary to the early region of JCV and BKV genome (AgT). The presence of JC virus or BK virus were confirmed by two other PCR assays using sets of primers complementary to the VP1 gene of each virus. Analysis of the data was performed by the Kruskal-Wallis test for numerical data and Pearson or Yates for categorical variables. RESULTS: A total of 75 patients were included in the study. The overall prevalence of polyomavirus DNA urinary shedding was 67/75 (89.3%). Only BKV DNA was detected in 14/75 (18.7%) urine samples, and only JCV DNA was detected in 11/75 (14.7%) samples. Both BKV and JCV DNA were present in 42/75 (56.0%) samples. CONCLUSION: In this study we found high rates of excretion of JCV, BKV, and simultaneous excretion in HIV+ patients. Also these results differ from the others available on the literature. |
format |
Article in Journal/Newspaper |
author |
Luiz Henrique da Silva Nali Cristiane de Campos Centrone Paulo Roberto Palma Urbano Augusto César Penalva-de-Oliveira Jose Ernesto Vidal Erique Peixoto Miranda Claudio Sérgio Pannuti Maria Cristina Domingues da Silva Fink |
author_facet |
Luiz Henrique da Silva Nali Cristiane de Campos Centrone Paulo Roberto Palma Urbano Augusto César Penalva-de-Oliveira Jose Ernesto Vidal Erique Peixoto Miranda Claudio Sérgio Pannuti Maria Cristina Domingues da Silva Fink |
author_sort |
Luiz Henrique da Silva Nali |
title |
High prevalence of the simultaneous excretion of polyomaviruses JC and BK in the urine of HIV-infected patients without neurological symptoms in São Paulo, Brazil |
title_short |
High prevalence of the simultaneous excretion of polyomaviruses JC and BK in the urine of HIV-infected patients without neurological symptoms in São Paulo, Brazil |
title_full |
High prevalence of the simultaneous excretion of polyomaviruses JC and BK in the urine of HIV-infected patients without neurological symptoms in São Paulo, Brazil |
title_fullStr |
High prevalence of the simultaneous excretion of polyomaviruses JC and BK in the urine of HIV-infected patients without neurological symptoms in São Paulo, Brazil |
title_full_unstemmed |
High prevalence of the simultaneous excretion of polyomaviruses JC and BK in the urine of HIV-infected patients without neurological symptoms in São Paulo, Brazil |
title_sort |
high prevalence of the simultaneous excretion of polyomaviruses jc and bk in the urine of hiv-infected patients without neurological symptoms in são paulo, brazil |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2012 |
url |
https://doi.org/10.1590/S0036-46652012000400004 https://doaj.org/article/b4081ae3a815454194a8cefd510e06fc |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 54, Iss 4, Pp 201-205 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652012000400004&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652012000400004 https://doaj.org/article/b4081ae3a815454194a8cefd510e06fc |
op_doi |
https://doi.org/10.1590/S0036-46652012000400004 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
54 |
container_issue |
4 |
container_start_page |
201 |
op_container_end_page |
205 |
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