Schistosoma mansoni venom allergen like proteins present differential allergic responses in a murine model of airway inflammation.
BACKGROUND: The Schistosoma mansoni Venom-Allergen-Like proteins (SmVALs) are members of the SCP/TAPS (Sperm-coating protein/Tpx-1/Ag5/PR-1/Sc7) protein superfamily, which may be important in the host-pathogen interaction. Some of these molecules were suggested by us and others as potential immunomo...
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ftdoajarticles:oai:doaj.org/article:b3fb8758bccf40879f183d71d69c14da 2023-05-15T15:14:20+02:00 Schistosoma mansoni venom allergen like proteins present differential allergic responses in a murine model of airway inflammation. Leonardo Paiva Farias Dunia Rodrigues Vinicius Cunna Henrique Krambeck Rofatto Eliana L Faquim-Mauro Luciana C C Leite 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001510 https://doaj.org/article/b3fb8758bccf40879f183d71d69c14da EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3274501?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001510 https://doaj.org/article/b3fb8758bccf40879f183d71d69c14da PLoS Neglected Tropical Diseases, Vol 6, Iss 2, p e1510 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001510 2022-12-31T05:35:48Z BACKGROUND: The Schistosoma mansoni Venom-Allergen-Like proteins (SmVALs) are members of the SCP/TAPS (Sperm-coating protein/Tpx-1/Ag5/PR-1/Sc7) protein superfamily, which may be important in the host-pathogen interaction. Some of these molecules were suggested by us and others as potential immunomodulators and vaccine candidates, due to their functional classification, expression profile and predicted localization. From a vaccine perspective, one of the concerns is the potential allergic effect of these molecules. METHODOLOGY/PRINCIPAL FINDINGS: Herein, we characterized the putative secreted proteins SmVAL4 and SmVAL26 and explored the mouse model of airway inflammation to investigate their potential allergenic properties. The respective recombinant proteins were obtained in the Pichia pastoris system and the purified proteins used to produce specific antibodies. SmVAL4 protein was revealed to be present only in the cercarial stage, increasing from 0-6 h in the secretions of newly transformed schistosomulum. SmVAL26 was identified only in the egg stage, mainly in the hatched eggs' fluid and also in the secretions of cultured eggs. Concerning the investigation of the allergic properties of these proteins in the mouse model of airway inflammation, SmVAL4 induced a significant increase in total cells in the bronchoalveolar lavage fluid, mostly due to an increase in eosinophils and macrophages, which correlated with increases in IgG1, IgE and IL-5, characterizing a typical allergic airway inflammation response. High titers of anaphylactic IgG1 were revealed by the Passive Cutaneous Anaphylactic (PCA) hypersensitivity assay. Additionally, in a more conventional protocol of immunization for vaccine trials, rSmVAL4 still induced high levels of IgG1 and IgE. CONCLUSIONS: Our results suggest that members of the SmVAL family do present allergic properties; however, this varies significantly and therefore should be considered in the design of a schistosomiasis vaccine. Additionally, the murine model of airway inflammation ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 2 e1510 |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Leonardo Paiva Farias Dunia Rodrigues Vinicius Cunna Henrique Krambeck Rofatto Eliana L Faquim-Mauro Luciana C C Leite Schistosoma mansoni venom allergen like proteins present differential allergic responses in a murine model of airway inflammation. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: The Schistosoma mansoni Venom-Allergen-Like proteins (SmVALs) are members of the SCP/TAPS (Sperm-coating protein/Tpx-1/Ag5/PR-1/Sc7) protein superfamily, which may be important in the host-pathogen interaction. Some of these molecules were suggested by us and others as potential immunomodulators and vaccine candidates, due to their functional classification, expression profile and predicted localization. From a vaccine perspective, one of the concerns is the potential allergic effect of these molecules. METHODOLOGY/PRINCIPAL FINDINGS: Herein, we characterized the putative secreted proteins SmVAL4 and SmVAL26 and explored the mouse model of airway inflammation to investigate their potential allergenic properties. The respective recombinant proteins were obtained in the Pichia pastoris system and the purified proteins used to produce specific antibodies. SmVAL4 protein was revealed to be present only in the cercarial stage, increasing from 0-6 h in the secretions of newly transformed schistosomulum. SmVAL26 was identified only in the egg stage, mainly in the hatched eggs' fluid and also in the secretions of cultured eggs. Concerning the investigation of the allergic properties of these proteins in the mouse model of airway inflammation, SmVAL4 induced a significant increase in total cells in the bronchoalveolar lavage fluid, mostly due to an increase in eosinophils and macrophages, which correlated with increases in IgG1, IgE and IL-5, characterizing a typical allergic airway inflammation response. High titers of anaphylactic IgG1 were revealed by the Passive Cutaneous Anaphylactic (PCA) hypersensitivity assay. Additionally, in a more conventional protocol of immunization for vaccine trials, rSmVAL4 still induced high levels of IgG1 and IgE. CONCLUSIONS: Our results suggest that members of the SmVAL family do present allergic properties; however, this varies significantly and therefore should be considered in the design of a schistosomiasis vaccine. Additionally, the murine model of airway inflammation ... |
format |
Article in Journal/Newspaper |
author |
Leonardo Paiva Farias Dunia Rodrigues Vinicius Cunna Henrique Krambeck Rofatto Eliana L Faquim-Mauro Luciana C C Leite |
author_facet |
Leonardo Paiva Farias Dunia Rodrigues Vinicius Cunna Henrique Krambeck Rofatto Eliana L Faquim-Mauro Luciana C C Leite |
author_sort |
Leonardo Paiva Farias |
title |
Schistosoma mansoni venom allergen like proteins present differential allergic responses in a murine model of airway inflammation. |
title_short |
Schistosoma mansoni venom allergen like proteins present differential allergic responses in a murine model of airway inflammation. |
title_full |
Schistosoma mansoni venom allergen like proteins present differential allergic responses in a murine model of airway inflammation. |
title_fullStr |
Schistosoma mansoni venom allergen like proteins present differential allergic responses in a murine model of airway inflammation. |
title_full_unstemmed |
Schistosoma mansoni venom allergen like proteins present differential allergic responses in a murine model of airway inflammation. |
title_sort |
schistosoma mansoni venom allergen like proteins present differential allergic responses in a murine model of airway inflammation. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001510 https://doaj.org/article/b3fb8758bccf40879f183d71d69c14da |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 2, p e1510 (2012) |
op_relation |
http://europepmc.org/articles/PMC3274501?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001510 https://doaj.org/article/b3fb8758bccf40879f183d71d69c14da |
op_doi |
https://doi.org/10.1371/journal.pntd.0001510 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
6 |
container_issue |
2 |
container_start_page |
e1510 |
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1766344802935242752 |