Palmelloid formation in the Antarctic psychrophile, Chlamydomonas priscuii, is photoprotective
Cultures of the obligate, Antarctic psychrophile, Chlamydomonas priscuii grown at permissive low temperature (8°C) are composed of flagellated, single cells, as well as non-motile, multicellular palmelloids. The relative proportions of the two cell types are temperature dependent. However, the tempe...
Published in: | Frontiers in Plant Science |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fpls.2022.911035 https://doaj.org/article/b3f843db51814508a4781bc3a3859a83 |
id |
ftdoajarticles:oai:doaj.org/article:b3f843db51814508a4781bc3a3859a83 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:b3f843db51814508a4781bc3a3859a83 2023-05-15T13:58:18+02:00 Palmelloid formation in the Antarctic psychrophile, Chlamydomonas priscuii, is photoprotective Beth Szyszka-Mroz Alexander G. Ivanov Charles G. Trick Norman P. A. Hüner 2022-08-01T00:00:00Z https://doi.org/10.3389/fpls.2022.911035 https://doaj.org/article/b3f843db51814508a4781bc3a3859a83 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fpls.2022.911035/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2022.911035 https://doaj.org/article/b3f843db51814508a4781bc3a3859a83 Frontiers in Plant Science, Vol 13 (2022) Chlamydomonas priscuii antarctic psychrophile palmelloids photoprotection temperature Plant culture SB1-1110 article 2022 ftdoajarticles https://doi.org/10.3389/fpls.2022.911035 2022-12-30T23:44:18Z Cultures of the obligate, Antarctic psychrophile, Chlamydomonas priscuii grown at permissive low temperature (8°C) are composed of flagellated, single cells, as well as non-motile, multicellular palmelloids. The relative proportions of the two cell types are temperature dependent. However, the temperature dependence for palmelloid formation is not restricted to psychrophilic C. priscuii but appears to be a general response of mesophilic Chlamydomonas species (C. reinhardtii and C. raudensis) to non-permissive growth temperatures. To examine potential differences in photosynthetic performance between single cells versus palmelloids of the psychrophile, a cell filtration technique was developed to separate single cells from palmelloids of C. priscuii grown at 8°C. Flow cytometry was used to estimate the diameter of isolated single cells (≤5 μm) versus isolated palmelloids of varying size (≥8 μm). Compared to single cells, palmelloids of C. priscuii showed a decrease in the abundance of light-harvesting complex II (LHCII) proteins with a 2-fold higher Chl a/b ratio. A decrease in both lutein and β-carotene in palmelloids resulted in carotenoid pools which were 27% lower in palmelloids compared to single cells of the psychrophile. Chlorophyll fluorescence analyses of the isolated fractions revealed that maximum photochemical efficiency of PSII (Fv/Fm) was comparable for both single cells and palmelloids of C. priscuii. However, isolated palmelloids exhibited lower excitation pressure, measured as 1 - qL, but higher yield of PSII (ΦPSII) and 50% higher rates of electron transport (ETR) than single cells exposed to high light at 8°C. This decreased sensitivity to high light in isolated palmelloids compared to single cells was associated with greater non-regulated dissipation of excess absorbed energy (ΦNO) with minimal differences in ΦNPQ in C. priscuii in response to increasing irradiance at low temperature. The ratio ΦNO/ΦNPQ observed for isolated palmelloids of C. priscuii developed at 8°C (1.414 ± 0.036) was ... Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Frontiers in Plant Science 13 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Chlamydomonas priscuii antarctic psychrophile palmelloids photoprotection temperature Plant culture SB1-1110 |
spellingShingle |
Chlamydomonas priscuii antarctic psychrophile palmelloids photoprotection temperature Plant culture SB1-1110 Beth Szyszka-Mroz Alexander G. Ivanov Charles G. Trick Norman P. A. Hüner Palmelloid formation in the Antarctic psychrophile, Chlamydomonas priscuii, is photoprotective |
topic_facet |
Chlamydomonas priscuii antarctic psychrophile palmelloids photoprotection temperature Plant culture SB1-1110 |
description |
Cultures of the obligate, Antarctic psychrophile, Chlamydomonas priscuii grown at permissive low temperature (8°C) are composed of flagellated, single cells, as well as non-motile, multicellular palmelloids. The relative proportions of the two cell types are temperature dependent. However, the temperature dependence for palmelloid formation is not restricted to psychrophilic C. priscuii but appears to be a general response of mesophilic Chlamydomonas species (C. reinhardtii and C. raudensis) to non-permissive growth temperatures. To examine potential differences in photosynthetic performance between single cells versus palmelloids of the psychrophile, a cell filtration technique was developed to separate single cells from palmelloids of C. priscuii grown at 8°C. Flow cytometry was used to estimate the diameter of isolated single cells (≤5 μm) versus isolated palmelloids of varying size (≥8 μm). Compared to single cells, palmelloids of C. priscuii showed a decrease in the abundance of light-harvesting complex II (LHCII) proteins with a 2-fold higher Chl a/b ratio. A decrease in both lutein and β-carotene in palmelloids resulted in carotenoid pools which were 27% lower in palmelloids compared to single cells of the psychrophile. Chlorophyll fluorescence analyses of the isolated fractions revealed that maximum photochemical efficiency of PSII (Fv/Fm) was comparable for both single cells and palmelloids of C. priscuii. However, isolated palmelloids exhibited lower excitation pressure, measured as 1 - qL, but higher yield of PSII (ΦPSII) and 50% higher rates of electron transport (ETR) than single cells exposed to high light at 8°C. This decreased sensitivity to high light in isolated palmelloids compared to single cells was associated with greater non-regulated dissipation of excess absorbed energy (ΦNO) with minimal differences in ΦNPQ in C. priscuii in response to increasing irradiance at low temperature. The ratio ΦNO/ΦNPQ observed for isolated palmelloids of C. priscuii developed at 8°C (1.414 ± 0.036) was ... |
format |
Article in Journal/Newspaper |
author |
Beth Szyszka-Mroz Alexander G. Ivanov Charles G. Trick Norman P. A. Hüner |
author_facet |
Beth Szyszka-Mroz Alexander G. Ivanov Charles G. Trick Norman P. A. Hüner |
author_sort |
Beth Szyszka-Mroz |
title |
Palmelloid formation in the Antarctic psychrophile, Chlamydomonas priscuii, is photoprotective |
title_short |
Palmelloid formation in the Antarctic psychrophile, Chlamydomonas priscuii, is photoprotective |
title_full |
Palmelloid formation in the Antarctic psychrophile, Chlamydomonas priscuii, is photoprotective |
title_fullStr |
Palmelloid formation in the Antarctic psychrophile, Chlamydomonas priscuii, is photoprotective |
title_full_unstemmed |
Palmelloid formation in the Antarctic psychrophile, Chlamydomonas priscuii, is photoprotective |
title_sort |
palmelloid formation in the antarctic psychrophile, chlamydomonas priscuii, is photoprotective |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fpls.2022.911035 https://doaj.org/article/b3f843db51814508a4781bc3a3859a83 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Frontiers in Plant Science, Vol 13 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fpls.2022.911035/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2022.911035 https://doaj.org/article/b3f843db51814508a4781bc3a3859a83 |
op_doi |
https://doi.org/10.3389/fpls.2022.911035 |
container_title |
Frontiers in Plant Science |
container_volume |
13 |
_version_ |
1766266532755668992 |