Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice.
Ascaris spp. infection affects 800 million people worldwide, and half of the world population is currently at risk of infection. Recurrent reinfection in humans is mostly due to the simplicity of the parasite life cycle, but the impact of multiple exposures to the biology of the infection and the co...
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ftdoajarticles:oai:doaj.org/article:b3c9b438bb1846d69faa0ce4283404a4 2023-05-15T15:06:56+02:00 Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice. Denise Silva Nogueira Pedro Henrique Gazzinelli-Guimarães Fernando Sérgio Barbosa Nathália Maria Resende Caroline Cavalcanti Silva Luciana Maria de Oliveira Chiara Cássia Oliveira Amorim Fabrício Marcus Silva Oliveira Matheus Silvério Mattos Lucas Rocha Kraemer Marcelo Vidigal Caliari Soraya Gaze Lilian Lacerda Bueno Remo Castro Russo Ricardo Toshio Fujiwara 2016-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004382 https://doaj.org/article/b3c9b438bb1846d69faa0ce4283404a4 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4729520?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004382 https://doaj.org/article/b3c9b438bb1846d69faa0ce4283404a4 PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004382 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004382 2022-12-31T00:34:17Z Ascaris spp. infection affects 800 million people worldwide, and half of the world population is currently at risk of infection. Recurrent reinfection in humans is mostly due to the simplicity of the parasite life cycle, but the impact of multiple exposures to the biology of the infection and the consequences to the host's homeostasis are poorly understood. In this context, single and multiple exposures in mice were performed in order to characterize the parasitological, histopathological, tissue functional and immunological aspects of experimental larval ascariasis. The most important findings revealed that reinfected mice presented a significant reduction of parasite burden in the lung and an increase in the cellularity in the bronchoalveolar lavage (BAL) associated with a robust granulocytic pulmonary inflammation, leading to a severe impairment of respiratory function. Moreover, the multiple exposures to Ascaris elicited an increased number of circulating inflammatory cells as well as production of higher levels of systemic cytokines, mainly IL-4, IL-5, IL-6, IL-10, IL-17A and TNF-α when compared to single-infected animals. Taken together, our results suggest the intense pulmonary inflammation associated with a polarized systemic Th2/Th17 immune response are crucial to control larval migration after multiple exposures to Ascaris. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 1 e0004382 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Denise Silva Nogueira Pedro Henrique Gazzinelli-Guimarães Fernando Sérgio Barbosa Nathália Maria Resende Caroline Cavalcanti Silva Luciana Maria de Oliveira Chiara Cássia Oliveira Amorim Fabrício Marcus Silva Oliveira Matheus Silvério Mattos Lucas Rocha Kraemer Marcelo Vidigal Caliari Soraya Gaze Lilian Lacerda Bueno Remo Castro Russo Ricardo Toshio Fujiwara Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Ascaris spp. infection affects 800 million people worldwide, and half of the world population is currently at risk of infection. Recurrent reinfection in humans is mostly due to the simplicity of the parasite life cycle, but the impact of multiple exposures to the biology of the infection and the consequences to the host's homeostasis are poorly understood. In this context, single and multiple exposures in mice were performed in order to characterize the parasitological, histopathological, tissue functional and immunological aspects of experimental larval ascariasis. The most important findings revealed that reinfected mice presented a significant reduction of parasite burden in the lung and an increase in the cellularity in the bronchoalveolar lavage (BAL) associated with a robust granulocytic pulmonary inflammation, leading to a severe impairment of respiratory function. Moreover, the multiple exposures to Ascaris elicited an increased number of circulating inflammatory cells as well as production of higher levels of systemic cytokines, mainly IL-4, IL-5, IL-6, IL-10, IL-17A and TNF-α when compared to single-infected animals. Taken together, our results suggest the intense pulmonary inflammation associated with a polarized systemic Th2/Th17 immune response are crucial to control larval migration after multiple exposures to Ascaris. |
format |
Article in Journal/Newspaper |
author |
Denise Silva Nogueira Pedro Henrique Gazzinelli-Guimarães Fernando Sérgio Barbosa Nathália Maria Resende Caroline Cavalcanti Silva Luciana Maria de Oliveira Chiara Cássia Oliveira Amorim Fabrício Marcus Silva Oliveira Matheus Silvério Mattos Lucas Rocha Kraemer Marcelo Vidigal Caliari Soraya Gaze Lilian Lacerda Bueno Remo Castro Russo Ricardo Toshio Fujiwara |
author_facet |
Denise Silva Nogueira Pedro Henrique Gazzinelli-Guimarães Fernando Sérgio Barbosa Nathália Maria Resende Caroline Cavalcanti Silva Luciana Maria de Oliveira Chiara Cássia Oliveira Amorim Fabrício Marcus Silva Oliveira Matheus Silvério Mattos Lucas Rocha Kraemer Marcelo Vidigal Caliari Soraya Gaze Lilian Lacerda Bueno Remo Castro Russo Ricardo Toshio Fujiwara |
author_sort |
Denise Silva Nogueira |
title |
Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice. |
title_short |
Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice. |
title_full |
Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice. |
title_fullStr |
Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice. |
title_full_unstemmed |
Multiple Exposures to Ascaris suum Induce Tissue Injury and Mixed Th2/Th17 Immune Response in Mice. |
title_sort |
multiple exposures to ascaris suum induce tissue injury and mixed th2/th17 immune response in mice. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004382 https://doaj.org/article/b3c9b438bb1846d69faa0ce4283404a4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004382 (2016) |
op_relation |
http://europepmc.org/articles/PMC4729520?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004382 https://doaj.org/article/b3c9b438bb1846d69faa0ce4283404a4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004382 |
container_title |
PLOS Neglected Tropical Diseases |
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10 |
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1 |
container_start_page |
e0004382 |
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