Occurrence of K1 and K2 serotypes and genotypic characteristics of extended spectrum β-lactamases-producing Klebsiella pneumoniae isolated from selected hospitals in Malaysia
Objective: To determine the distribution, phenotypic and genetic background of extended spectrum β-lactamases (ESBL)-producing Klebsiella (K.) pneumoniae clinical isolates associated with K1 and K2 serotypes in two selected hospitals in Malaysia. Methods: A total of 192 K. pneumoniae isolates were c...
Published in: | Asian Pacific Journal of Tropical Medicine |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2024
|
Subjects: | |
Online Access: | https://doi.org/10.4103/apjtm.apjtm_303_23 https://doaj.org/article/b3ad2284eccf45028a57c9b35685e86f |
Summary: | Objective: To determine the distribution, phenotypic and genetic background of extended spectrum β-lactamases (ESBL)-producing Klebsiella (K.) pneumoniae clinical isolates associated with K1 and K2 serotypes in two selected hospitals in Malaysia. Methods: A total of 192 K. pneumoniae isolates were collected and subjected to antibiotic susceptibility, hypermucoviscosity test and multiplex PCR to detect the presence of K1- and K2- serotype associated genes. Multilocus sequence typing (MLST) was performed on ESBL-producing K. pneumoniae isolates presented with K1 and K2 serotypes, followed by phylogenetic analysis. Results: A total of 87 out of 192 (45.3%) of the K. pneumoniae isolates collected were ESBL producers. However, only 8.3% (16/192) and 10.9% (21/192) of the total isolates were detected to carry K1- and K2-serotype associated genes, respectively. Statistical analysis showed that K1 and K2 capsular serotypes were not significantly associated with ESBL phenotype (P=0.196). However, they were significantly associated with hypervirulent, as demonstrated by the positive string test (P<0.001). MLST analysis revealed that ST23 as the predominant sequence type (ST) in the K1 serotype, while the ST in the K2 serotype is more diverse. Conclusions: Although the occurrence of ESBL-producing isolates among the hypervirulent strains was low, their coexistence warrants the need for continuous surveillance. MLST showed that these isolates were genetically heterogeneous. |
---|