Development of duplex-PCR for identification of Aeromonas species
Introduction The number of reports of intestinal infections caused by Aeromonas spp. has increased significantly in recent years. In most clinical laboratories, identification of these bacteria is carried out by general phenotypic tests that sometimes do not accurately differentiate Aeromonas and Vi...
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ftdoajarticles:oai:doaj.org/article:b3ab3fb6d36747ae96b702d8de6e7f74 2023-05-15T15:00:21+02:00 Development of duplex-PCR for identification of Aeromonas species Carina Lucena Mendes-Marques Ernesto Hofer Nilma Cintra Leal 2013-10-01T00:00:00Z https://doaj.org/article/b3ab3fb6d36747ae96b702d8de6e7f74 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013005000014 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/b3ab3fb6d36747ae96b702d8de6e7f74 Revista da Sociedade Brasileira de Medicina Tropical, Iss ahead, Pp 00-00 (2013) Aeromonas Identification PCR Arctic medicine. Tropical medicine RC955-962 article 2013 ftdoajarticles 2022-12-30T23:21:46Z Introduction The number of reports of intestinal infections caused by Aeromonas spp. has increased significantly in recent years. In most clinical laboratories, identification of these bacteria is carried out by general phenotypic tests that sometimes do not accurately differentiate Aeromonas and Vibrio. Methods A duplex-polymerase chain reaction (PCR) was developed directed to 2 targets identifying Aeromonas spp. pathogenic to humans. Results The duplex-PCR results were reproducible and specific for Aeromonas spp. pathogenic to humans. Conclusions This method will allow differentiation between Vibrio and Aeromonas spp. in patients with in cholera-like symptoms and can also be used in water quality monitoring. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Aeromonas Identification PCR Arctic medicine. Tropical medicine RC955-962 |
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Aeromonas Identification PCR Arctic medicine. Tropical medicine RC955-962 Carina Lucena Mendes-Marques Ernesto Hofer Nilma Cintra Leal Development of duplex-PCR for identification of Aeromonas species |
topic_facet |
Aeromonas Identification PCR Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction The number of reports of intestinal infections caused by Aeromonas spp. has increased significantly in recent years. In most clinical laboratories, identification of these bacteria is carried out by general phenotypic tests that sometimes do not accurately differentiate Aeromonas and Vibrio. Methods A duplex-polymerase chain reaction (PCR) was developed directed to 2 targets identifying Aeromonas spp. pathogenic to humans. Results The duplex-PCR results were reproducible and specific for Aeromonas spp. pathogenic to humans. Conclusions This method will allow differentiation between Vibrio and Aeromonas spp. in patients with in cholera-like symptoms and can also be used in water quality monitoring. |
format |
Article in Journal/Newspaper |
author |
Carina Lucena Mendes-Marques Ernesto Hofer Nilma Cintra Leal |
author_facet |
Carina Lucena Mendes-Marques Ernesto Hofer Nilma Cintra Leal |
author_sort |
Carina Lucena Mendes-Marques |
title |
Development of duplex-PCR for identification of Aeromonas species |
title_short |
Development of duplex-PCR for identification of Aeromonas species |
title_full |
Development of duplex-PCR for identification of Aeromonas species |
title_fullStr |
Development of duplex-PCR for identification of Aeromonas species |
title_full_unstemmed |
Development of duplex-PCR for identification of Aeromonas species |
title_sort |
development of duplex-pcr for identification of aeromonas species |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2013 |
url |
https://doaj.org/article/b3ab3fb6d36747ae96b702d8de6e7f74 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Iss ahead, Pp 00-00 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013005000014 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/b3ab3fb6d36747ae96b702d8de6e7f74 |
_version_ |
1766332448903266304 |